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Maulin Inggraini1 
, Noor Andryan Ilsan2 , Ni Putu Ratna Ayu Krishanti3 , Jepri Agung Priyanto4 , Reza Anindita5 , Fhasya Algina Hawatul Haq2 , Oktavia Dwi Mauria2 , Salsabila Ranmika Sari6 , Najla Salsabila Rahmadina7
, Noor Andryan Ilsan2 , Ni Putu Ratna Ayu Krishanti3 , Jepri Agung Priyanto4 , Reza Anindita5 , Fhasya Algina Hawatul Haq2 , Oktavia Dwi Mauria2 , Salsabila Ranmika Sari6 , Najla Salsabila Rahmadina7
1- Department of Medical Laboratory Technology, STIKes Mitra Keluarga, Pengasinan Street, Bekasi City, West Java, Indonesia , maulin.inggraini@stikesmitrakeluarga.ac.id
2- Department of Medical Laboratory Technology, STIKes Mitra Keluarga, Pengasinan Street, Bekasi City, West Java, Indonesia
3- Research Center for Applied Zoology, National Research and Innovation Agency, Soekarno Science and Techno Park, Jl. Raya Bogor KM 46, Cibinong, Bogor, West Java Province 16911, Indonesia
4- Division of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University (IPB University), Bogor, Dramaga, Indonesia
5- Department of Pharmacy, STIKes Mitra Keluarga, Pengasinan Street, Bekasi City, West Java, Indonesia
6- Department of Nursing, STIKes Mitra Keluarga, Pengasinan Street, Bekasi City, West Java, Indonesia
7- Department of Nutrition, STIKes Mitra Keluarga, Pengasinan Street, Bekasi City, West Java, Indonesia
2- Department of Medical Laboratory Technology, STIKes Mitra Keluarga, Pengasinan Street, Bekasi City, West Java, Indonesia
3- Research Center for Applied Zoology, National Research and Innovation Agency, Soekarno Science and Techno Park, Jl. Raya Bogor KM 46, Cibinong, Bogor, West Java Province 16911, Indonesia
4- Division of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University (IPB University), Bogor, Dramaga, Indonesia
5- Department of Pharmacy, STIKes Mitra Keluarga, Pengasinan Street, Bekasi City, West Java, Indonesia
6- Department of Nursing, STIKes Mitra Keluarga, Pengasinan Street, Bekasi City, West Java, Indonesia
7- Department of Nutrition, STIKes Mitra Keluarga, Pengasinan Street, Bekasi City, West Java, Indonesia
Abstract: (77 Views)
Background and Objective: One of the animal models for bacterial virulence testing is Omphisa fuscidentalis. The purpose of this study was to evaluate Omphisa fuscidentalis larvae as an animal model for bacterial virulence testing.
Methods: The design of this study was experimental. Bacterial samples were obtained from Mitra Keluarga Hospital, Bekasi, Indonesia. Molecular Identification of Bacteria Using Partial 16S rRNA Gene Sequencing. Identification And Susceptibility Testing Of Bacteria Using Vitek 2 Compact. The dose treatment of bacterial isolates Staphylococcus haemolyticus, Enterococcus faecalis, and Klebsiella pneumoniae 103, 104, 105 was carried out on the survivability of O. fuscidentalis larvae over 24 hours post-injection. Data analysis was descriptive quantitative.
Results: K. pneumoniae strains were resistant to more than five classes of antibiotics. The lowest survival percentage was obtained in O. fuscidentalis larvae injected with K. pneumoniae. Microscopic observation of the hemocytes of dead O. fuscidentalis larvae showed swelling and increased cell size compared to healthy hemocytes from control larvae injected with sterile phosphate-buffered saline.
Conclusion: O. fuscidentalis larvae are a promising alternative model for assessing bacterial virulence.
Keywords : Virulence, Omphisa fuscidentalis, Animal model, Multi-drug resistant, Hemocytes, Galleria mellonella
Keywords: Virulence, Omphisa fuscidentalis, Animal model, Multi-drug resistant, Hemocytes, Galleria mellonella
Type of Study: Original Research Article |
Subject:
Medical Bacteriology
Received: 2025/04/22 | Accepted: 2025/07/27 | ePublished: 2025/08/18
Received: 2025/04/22 | Accepted: 2025/07/27 | ePublished: 2025/08/18
References
1. Kaito C, Murakami K, Imai L, Furuta K. Animal infection models using non-mammals. Microbiol Immunol. 2020;64(9):585-92. [DOI:10.1111/1348-0421.12834] [PMID] [PMCID]
2. Kay S, Edwards J, Brown J, Dixon R. Galleria mellonella Infection Model Identifies Both High and Low Lethality of Clostridium perfringens Toxigenic Strains and Their Response to Antimicrobials. Front Microbiol. 2019;10:1281. [DOI:10.3389/fmicb.2019.01281] [PMID] [PMCID]
3. Tsai CJ, Loh JM, Proft T. Galleria mellonella infection models for the study of bacterial diseases and for antimicrobial drug testing. Virulence. 2015;7(3):214-29. [DOI:10.1080/21505594.2015.1135289] [PMID] [PMCID]
4. Ten KE, Muzahid NH, Rahman S, Tan HS. Use of the waxworm Galleria mellonella larvae as an infection model to study Acinetobacter baumannii. PLoS One. 2023;18(4):e0283960. [DOI:10.1371/journal.pone.0283960] [PMID] [PMCID]
5. Villani S, Calcagnile M, Demitri C, Alifano P. Galleria mellonella (Greater Wax Moth) as a Reliable Animal Model to Study the Efficacy of Nanomaterials in Fighting Pathogens. Nanomaterials (Basel). 2025;15(1):67. [DOI:10.3390/nano15010067] [PMID] [PMCID]
6. Ignasiak K, Maxwell A. Galleria mellonella (greater wax moth) larvae as a model for antibiotic susceptibility testing and acute toxicity trials. BMC Res Notes. 2017;10(1):428. [DOI:10.1186/s13104-017-2757-8] [PMID] [PMCID]
7. Andrea A, Krogfelt KA, Jenssen H. Methods and Challenges of Using the Greater Wax Moth (Galleria mellonella) as a Model Organism in Antimicrobial Compound Discovery. Microorganisms. 2019;7(3):85. [DOI:10.3390/microorganisms7030085] [PMID] [PMCID]
8. Cools F, Torfs E, Aizawa J, Vanhoutte B, Maes L, Caljon G, et al. Optimization and Characterization of a Galleria mellonella Larval Infection Model for Virulence Studies and the Evaluation of Therapeutics Against Streptococcus pneumoniae. Front Microbiol. 2019;10:311. [DOI:10.3389/fmicb.2019.00311] [PMID] [PMCID]
9. Sułek M, Kordaczuk J, Mak P, Śmiałek-Bartyzel J, Hułas-Stasiak M, Wojda I. Immune priming modulates Galleria mellonella and Pseudomonas entomophila interaction. Antimicrobial properties of Kazal peptide Pr13a. Front Immunol. 2024;15:1358247. [DOI:10.3389/fimmu.2024.1358247] [PMID] [PMCID]
10. Wright CL, Kavanagh O. Galleria mellonella as a novel in vivo model to screen natural product-derived modulators of innate immunity. Appl Sci. 2022;12(13):6587. [DOI:10.3390/app12136587]
11. Firacative C, Khan A, Duan S, Ferreira-Paim K, Leemon D, Meyer W. Rearing and Maintenance of Galleria mellonella and Its Application to Study Fungal Virulence. J Fungi (Basel). 2020;6(3):130. [DOI:10.3390/jof6030130] [PMID] [PMCID]
12. Inggraini M, Ilsan NA, Romadhona VA, Anindita R. Pengujian Kemampuan Larva Ulat Bambu (Omphisa fuscidentalis) sebagai Hewan Uji Virulensi Bakteri Klebsiella pneumoniae. J Bioshell. 2023;12(2):134-40. [DOI:10.56013/bio.v12i2.2317]
13. Ilsan NA, Inggrain M, Nurfajriah S, Yunita M, Priyanto JA, Ramanda V. Virulence evaluation of Aeromonas spp. KS-1 isolated from kitchen sponge using Omphisa fuscidentalis larvae. Hayati J Biosciences. 2024;31(4):613-20. [DOI:10.4308/hjb.31.4.613-620]
14. Ilsan NA, Yunita M, Dewi NK, Irham LM, Sipriyadi, Nurfajriah S, et al. Potentially virulent multi-drug resistant Escherichia fergusonii isolated from inanimate surface in a medical university: Omphisa fuscidentalis as an alternative for bacterial virulence determination. Diagnostics. 2023;13(2):279. [DOI:10.3390/diagnostics13020279] [PMID] [PMCID]
15. Ilsan NA, Nurfajriah S, Inggraini M, Krishanti NPRA, Yunita M, Sipriyadi, et al. Application of miniaturized most probable number method for bacterial detection in water samples: detection of multi-drug-resistant Ralstonia insidiosa in drinking water. J Water Health. 2024;22(9):1618-27. [DOI:10.2166/wh.2024.086] [PMID]
16. Marchesi JR, Sato T, Weightman AJ, Martin TA, Fry JC, Hiom SJ, et al. Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol. 1998;64(2):795-9. [DOI:10.1128/AEM.64.2.795-799.1998] [PMID] [PMCID]
17. Ménard G, Rouillon A, Cattoir V, Donnio P-Y. Galleria mellonella as a suitable model of bacterial infection: past, present and future. Front Cell Infect Microbiol. 2021;11:782733. [DOI:10.3389/fcimb.2021.782733] [PMID] []
18. Bzazou El Ouazzani ZE, Benaicha H, Reklaoui L, Alloudane R, Barrijal S. First detection of colistin resistance encoding gene mcr-1 in clinical enterobacteriaceae isolates in Morocco. Iran J Med Microbiol. 2024;18(1):33-40. [DOI:10.30699/ijmm.18.1.33]
19. Mahdi Oraibi S. Klebsiella pneumoniae, Staphylococcus aureus and Proteus mirabilis Virulence Factors Detected from Hospitals in Nasiriyah, Iraq, 2022. Iran J Med Microbiol. 2024;18(3):209-13. [DOI:10.30699/ijmm.18.3.209]
20. Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG, et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 2012;18(3):268-81. [DOI:10.1111/j.1469-0691.2011.03570.x] [PMID]
21. Admella J, Torrents E. Investigating bacterial infections in Galleria mellonella larvae: Insights into pathogen dissemination and behavior. J Invertebr Pathol. 2023;200:107975. [DOI:10.1016/j.jip.2023.107975] [PMID]
22. Pereira TC, de Barros PP, Fugisaki LRO, Rossoni RD, Ribeiro FC, de Menezes RT, et al. Recent Advances in the Use of Galleria mellonella Model to Study Immune Responses against Human Pathogens. J Fungi (Basel). 2018;4(4):128. [DOI:10.3390/jof4040128] [PMID] [PMCID]
23. Quansah E, Ramoji A, Thieme L, Mirza K, Goering B, Makarewicz O, et al. Label-free multimodal imaging of infected Galleria mellonella larvae. Sci Rep. 2022;12(1):20416. [DOI:10.1038/s41598-022-24846-7] [PMID] [PMCID]
24. Shubin AV, Demidyuk IV, Komissarov AA, Rafieva LM, Kostrov SV. Cytoplasmic vacuolization in cell death and survival. Oncotarget. 2016;7(34):55863-89. [DOI:10.18632/oncotarget.10150] [PMID] [PMCID]
25. Kazek M, Kaczmarek A, Wrońska AK, Boguś MI. Conidiobolus coronatus induces oxidative stress and autophagy response in Galleria mellonella larvae. PLoS One. 2020;15(2):e0228407. [DOI:10.1371/journal.pone.0228407] [PMID] [PMCID]
26. Eltwisy HO, Twisy HO, Hafez MH, Sayed IM, El-Mokhtar MA. Clinical Infections, Antibiotic Resistance, and Pathogenesis of Staphylococcus haemolyticus. Microorganisms. 2022;10(6). [DOI:10.3390/microorganisms10061130] [PMID] [PMCID]
27. Bryce AN, Doocey R, Handy R. Staphylococcus haemolyticus meningitis and bacteremia in an allogenic stem cell transplant patient. IDCases. 2021;26:e01259. [DOI:10.1016/j.idcr.2021.e01259] [PMID] [PMCID]
28. Panda S, Singh DV. Biofilm Formation by ica-Negative Ocular Isolates of Staphylococcus haemolyticus. Front Microbiol. 2018;9:2687. [DOI:10.3389/fmicb.2018.02687] [PMID] [PMCID]
29. Cave R, Misra R, Chen J, Wang S, Mkrtchyan HV. Whole genome sequencing revealed new molecular characteristics in multidrug resistant staphylococci recovered from high frequency touched surfaces in London. Sci Rep. 2019;9(1):9637. [DOI:10.1038/s41598-019-45886-6] [PMID] [PMCID]
30. Pindar C, Viau RA. Staphylococcus haemolyticus epididymo-orchitis and bacteraemia: a case report. JMM Case Rep. 2018;5(7):e005157. [DOI:10.1099/jmmcr.0.005157] [PMID] [PMCID]
31. Mishra B, Khader R, Felix LO, Frate M, Mylonakis E, Meschwitz S, et al. A Substituted Diphenyl Amide Based Novel Scaffold Inhibits Staphylococcus aureus Virulence in a Galleria mellonella Infection Model. Front Microbiol. 2021;12:723133. [DOI:10.3389/fmicb.2021.723133] [PMID] [PMCID]
32. Sheehan G, Tully L, Kavanagh KA. Candida albicans increases the pathogenicity of Staphylococcus aureus during polymicrobial infection of Galleria mellonella larvae. Microbiology (Reading). 2020;166(4):375-85. [DOI:10.1099/mic.0.000892] [PMID] [PMCID]
33. Lozoya-Pérez NE, García-Carnero LC, Martínez-Álvarez JA, Martínez-Duncker I, Mora-Montes HM. Tenebrio molitor as an Alternative Model to Analyze the Sporothrix Species Virulence. Infect Drug Resist. 2021;14:2059-72. [DOI:10.2147/IDR.S312553] [PMID] [PMCID]
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