year 7, Issue 4 (Winter 2014)                   Iran J Med Microbiol 2014, 7(4): 9-15 | Back to browse issues page

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Abdi H A, rashki A, rashki Z, shahkarami F, shahraki Z. The relationship between phylogenetic group and distribution of virulence genes hlyA, iroN, iucD, fimH in Escherichia coli isolated from female genital tract among women attending Gynecology clinics in Zabol-Iran by Multiplex-PCR.. Iran J Med Microbiol. 2014; 7 (4) :9-15
URL: http://ijmm.ir/article-1-255-en.html
1- Faculty of Basic science
2- faculty Vet-medicin , ah_rashki@usal.es
3- faculty of medicine
4- faculty of vet-medicine
5- faculty of medecine
Abstract:   (15478 Views)
Background: The ability of adherence to the host cell surface, present of sidrophore receptors and α-hemolysin (hlyA) are common virulence attributes produced by Escherichia coli that enhances virulence in a number of clinical infections. It has been revealed that E. coli strains that infect uro-genital tracts have different virulence factors. This study was aimed to determine the prevalence of genes encoding virulence factors in cervico-vaginal pathogenic E. coli isolated from women attending Gynecology clinics in Zabol-Iran.
Materials and Methods: In this study, 380 cervico-vaginal swabs were obtained from patients with genital tract infections referred to Gynecology clinics in Zabol-Iran during the period (from January to July 2013). A total 132 E. coli isolates were confirmed by conventional biochemical tests. DNA was extracted from all isolates by the boiling method and then DNA was used to determine the presence and prevalence of selected virulence genes including hlyA, iroN, iucD and fimH. The phylo-groups of isolates were determined by detection of yjaA and chuA genes and fragment TspE4.C2.
Results: In 132 samples, the frequency of hlyA, iroN, iucD and fimH genes were 71, 37, 31 and 17% respectively. Four major phylogenetic lineages (A, B1, B2, and D) were found in 7%, 19%, 62% and 14% of isolates, respectively. Isolates present in group B2 showed highest presence of virulence genes.
Conclusion: This results show that the iroN, iucD, and fimH genes were the most virulent genes of E. coli isolates obtained from patients with uro-genital tract infection. These findings can be valuable in etiology of cervico-vaginal infections (CVIs), CVI administration and management and success of treatment strategies.
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Type of Study: Original | Subject: Molecular Microbiology
Received: 2014/03/11 | Accepted: 2014/05/25

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