Antibiotic Resistance Pattern and Prevalence of Virulence Factors among ESBLs-producing Escherichia coli Isolates Causing Urinary Tract Infections

Kazemi, Samaneh; Mojtahedi, Ali; Hosseini, Farzaneh

doi:10.30699/ijmm.17.6.629

year 17, Issue 6 (November - December 2023) Iran J Med Microbiol 2023, 17(6): 629-637 | Back to browse issues page

‎ 10.30699/ijmm.17.6.629

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Kazemi S, Mojtahedi A, Hosseini F. Antibiotic Resistance Pattern and Prevalence of Virulence Factors among ESBLs-producing Escherichia coli Isolates Causing Urinary Tract Infections. Iran J Med Microbiol 2023; 17 (6) :629-637
URL: http://ijmm.ir/article-1-2241-en.html

Antibiotic Resistance Pattern and Prevalence of Virulence Factors among ESBLs-producing Escherichia coli Isolates Causing Urinary Tract Infections

Samaneh Kazemi¹

, Ali Mojtahedi

², Farzaneh Hosseini¹

1- Department of Microbiology, School of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
2- Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran , mojtahedi.a@gmail.com

Abstract: (426 Views)

Background and Aim: Urinary tract infections (UTIs) pose a significant global health threat, primarily attributed to bacteria producing extended-spectrum beta-lactamases (ESBLs), with limited treatment options available. The specific genotypes of ESBL-producing strains remain largely unexplored in Guilan (northern Iran). Hence, the objective of this study was to determine the prevalence of ESBL-producing extra-intestinal pathogenic Escherichia coli (ExPEC) among hospitalized patients in this area.
Materials and Methods: In this descriptive cross-sectional study, 269 isolates were examined phenotypically for ESBL production. All ESBLs producing isolates were screened for the presence of virulence and housekeeping genes by polymerase chain reaction (PCR) using specific primers. The antimicrobial susceptibility patterns were determined using the disk diffusion method according to the CLSI recommendation.
Results: Out of the 269 E. coli isolates, 107 cases (39.8%) were identified as producers of ESBLs. Antibiotic susceptibility testing conducted on the ESBL-producing isolates revealed the highest levels of resistance to cefotaxime, ampicillin, amoxicillin-clavulanate, cephalothin, cefixime, and ceftriaxone (100.0%). Nalidixic acid exhibited a resistance rate of 91.6%, followed by ceftazidime at 84.1%. On the other hand, the isolates showed the highest susceptibility to imipenem (94.4%), followed by nitrofurantoin (87.9%) and gentamicin (63.9%).
Conclusion: The frequency of ESBL-producing ExPEC isolated from UTIs is concerning in Guilan. The most suitable antibiotic with the lowest risk of resistance for the patient can be prescribed using the updated susceptibility and resistance patterns of the bacteria.

Keywords: Escherichia coli, Extended spectrum beta-lactamases (ESBLs), Antibiotic Resistance, Virulence, Urinary Tract Infections (UTIs)

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Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2023/09/12 | Accepted: 2024/01/20 | ePublished: 2024/01/29

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