Iranian Journal of Medical Microbiology

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Gholami Parizad E, Gholami Parizad E, Delpisheh A, Nikfar M. Determination of HBV-DNA copies in serum and cerumen in chronic hepatitis B patien (CHB)by RealTtime PCR method and Its relationship with some epidemiological variables. Iran J Med Microbiol 2012; 5 (4) :42-51
URL: http://ijmm.ir/article-1-207-en.html

Background: Chronic hepatitis B is most common chronic viral infection in the human. Nearly a third of the world already infected with hepatitis B were more than four hundred million people worldwide are HBV carriers, and of these approximately forty million people and cirrhosis of the liver due to the sixty million people have died hepatocellular carcinoma. Outbreaks in Southeast Asia than other regions further. Is estimated that so far twenty -five million people in Iran are infected HBV infection and about three percent of HBV carriers exist in Iranian society. HBV-DNA levels in different body fluids including serum, cerumen, saliva and other immunological factors, individual and depends on some epidemiological variables. The first study to determine the amount of HBV-DNA copies in serum and cerumen its relationship with epidemiologic factors in Iran population.
Methods: In this study, 70 patients with chronic hepatitis B in the age group 20 - 40years, all were positive for HBsAg were selected as samples. In the study were used to determine the amount of HBV-DNA serum and cerumen by molecular methods Real time PCR (detection system Biorad) and diagnostic kits (aj Roboscreen - Germany). Results: In this study, 54.3 percent of male study group and 45.7 percent were women. 100 percent of patients at least one year of diagnosis Abtla’shan to hepatitis B was passed. 61 ( 87.2%) of patients had HBV-DNA to cerumen. 19 (27%) and 51 treated patients (73 percent) had not received any medication. 58 (83%) of patients were married. Average copies ml HBV-DNA has been in men cerumen 8.73×10⁶ and women's 7.44× 10⁶ . Hmogenety test average variance in age groups showed a significant difference between the mean copies cerumen the age groups studied there
Conclusion: The use of quantitative molecular methods can conditions CHB patients in different stages of disease to determine accurately. Type of discharge the patient, including serum, Srvmn, saliva, etc. as well as some epidemiological variables such as sex, drugs, and immune system. The amount of HBV-DNA copies are affected, though some might consider testing the statistical differences are not significant