Genomic Detection of Brucella Abortus in Milk obtained from Farms in Lorestan Province using PCR Method

Tahmasebi, Rahim; Jaydari, Amin; Shams, Nemat; Rahimi, Heidar

doi:10.30699/ijmm.16.5.479

year 16, Issue 5 (September - October 2022) Iran J Med Microbiol 2022, 16(5): 479-484 | Back to browse issues page

‎ 10.30699/ijmm.16.5.479

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Tahmasebi R, Jaydari A, Shams N, Rahimi H. Genomic Detection of Brucella Abortus in Milk obtained from Farms in Lorestan Province using PCR Method. Iran J Med Microbiol 2022; 16 (5) :479-484
URL: http://ijmm.ir/article-1-1724-en.html

Genomic Detection of Brucella Abortus in Milk obtained from Farms in Lorestan Province using PCR Method

Rahim Tahmasebi¹

, Amin Jaydari

², Nemat Shams¹

, Heidar Rahimi¹

1- Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorram Abad, Iran
2- Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorram Abad, Iran , jaydari.a@lu.ac.ir

Abstract: (987 Views)

Background and Aim: Brucellosis is a zoonotic disease which has become endemic in Iran. Contaminated milk with Brucella bacteria is the main way of transmission of this disease in humans. Therefore, the aim of this study was to investigate the prevalence of Brucella spp. and B. abortus in raw milk samples collected from farms belongs to six geographical areas of Lorestan province.
Materials and Methods: In the present study, 100 raw milk samples of cows which were less than 4 years, 4 to 6 years and over 6 years old were randomly collected. The isolates were identified by PCR method using specific bcsp31 and IS711 primers for Brucella spp. and B. abortus, respectively.
Results & Conclusion: The results showed that 26 of the samples were infected with Brucella bacteria, of which 19 samples (73%) were B. abortus. Most of the brucellosis infection in cows belonged to cows less than 4 years (31.4%) and 4 to 6 years (31.4%) categories, 11 samples in both groups, so that in cows older than 6 years this rate was 13% (4 samples). The east of the province with 12 samples and the northwest of the province with one sample showed the highest and lowest levels of Brucella infection, respectively. The findings demonstrated that there is a significant difference between the frequency of Brucella contamination in milk in the eastern regions of Lorestan province with other regions (p<0.05). It should be noted that the possibility of transmitting brucellosis to humans through consumption of contaminated raw milk and dairy products in Lorestan province is high.

Keywords: B. abortus, zoonosis, milk, cattle, PCR

Full-Text [PDF 575 kb] (393 Downloads) | | Full-Text (HTML) (668 Views)

Type of Study: Brief Original Article | Subject: Molecular Microbiology
Received: 2022/04/14 | Accepted: 2022/06/26 | ePublished: 2022/08/8

References

1. Taleski V, Zerva L, Kantardjiev T, Cvetnic Z, Erski-Biljic M, Nikolovski B, et al. An overview of the epidemiology and epizootology of brucellosis in selected countries of Central and Southeast Europe. Vet Microbiol. 2002;90(1-4):147-55. [DOI:10.1016/S0378-1135(02)00250-X]

2. Dadar M, Alamian S, Behrozikhah AM, Yazdani F, Kalantari A, Etemadi A, et al. Molecular identification of Brucella species and biovars associated with animal and human infection in Iran. Vet Res forum an Int Q J. 2019;10(4):315-21.

3. Golshani M, Buozari S. A review of Brucellosis in Iran: Epidemiology, Risk Factors, Diagnosis, Control, and Prevention. Iran Biomed J. 2017;21 (6):349-59.

4. Rahimi H, Tukmechi A, Rashidian E. Use of touch-down polymerase chain reaction to enhance the sensitivity of Brucella melitensis detection in raw milk. Anim Biotechnol. 2022;33(1):104-9. [DOI:10.1080/10495398.2020.1777149] [PMID]

5. Baily GG, Krahn JB, Drasar BS, Stoker NG. Detection of Brucella melitensis and Brucella abortus by DNA amplification. J Trop Med Hyg. 1992;95(4): 271-5.

6. Bricker BJ, Halling SM. Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol. 1994;32(11):2660-6. [DOI:10.1128/jcm.32.11.2660-2666.1994] [PMID] [PMCID]

7. Kashfi SM, Hatamian N, Rakhshani T. Epidemiological Study of the Brucellosis in Iran, Andimeshk, 2001-2016. J Heal Sci Surveill Syst. 2018;6(1):23-8.

8. Behroozikhah AM, Bagheri Nejad R, Amiri K, Bahonar AR. Identification at Biovar Level of Brucella Isolates Causing Abortion in Small Ruminants of Iran. Wong H-C, editor. J Pathog. 2012;2012:357235. [DOI:10.1155/2012/357235] [PMID] [PMCID]

9. Pishva E, Salehi R, Hoseini A, Kargar A, Taba FE, Hajiyan M, et al. Molecular typing of Brucella species isolates from Human and livestock bloods in Isfahan province. Adv Biomed Res. 2015;4:104.

10. Ashrafganjooyi SH, Saedadeli N, Alamian S, Khalili M, Shirazi Z. Isolation and biotyping of Brucella spp. from sheep and goats raw milk in southeastern Iran. Trop Biomed. 2017;34(3):507-11.

11. Azadi Chegeni S, Jaydari A. Seroprevalence of Malta Fever in Veterinary Staff of Lorestan Province Using Indirect ELISA Method in 2018-2019. Yafteh. 2020 ;22(2):36-42.

12. Abdali F, Hosseinzadeh S, Berizi E, Pourmontaseri M. Prevalence of Brucella species in unpasteurized dairy products consumed in Shiraz province using PCR assay. Mol Biol Res Commun. 2020;9(3):117-21.

13. Khalili M, Aflatoonian MR, Aliabadi FS, Abshenas J. Brucella contamination in raw milk by polymerase chain reaction. Tehran Univ Med J. 2016;74(7):517-21.

14. Shams N, Jaidari A, Etemadfar L. Molecular Detection of Brucella abortus and Brucella melitensis in Raw and Unpasteurized Bulk Cow Milk Tanks of Traditional Domestic Dairy Sale Centres in Khorramabad. Iran J Med Microbiol. 2017;11(4):13-20.

15. Shakerian A. Study of contamination rate in craw milk and its traditional products with Brucella abortus, and Brucella mellitensis in Isfahan and Chaharmahal and Bakhtiari Provinces. J Shahrekord Univ Med Sci. 2015;17(1):16-23.

16. Entezari M, Sepahvand S. Investigating Geographical Factors Affecting the Prevalence of Brucellosis in the Lorestan Province, Iran. J Isfahan Med Sch. 2014; 32(283): 569-79.

17. Abdalla A, Hamid ME. Comparison of conventional and non-conventional techniques for the diagnosis of bovine brucellosis in Sudan. Trop Anim Health Prod. 2012;44(6): 1151-5. [DOI:10.1007/s11250-011-0051-7] [PMID]

18. Muendo EN, Mbatha PM, Macharia J, Abdoel TH, Janszen P V, Pastoor R, et al. Infection of cattle in Kenya with Brucella abortus biovar 3 and Brucella melitensis biovar 1 genotypes. Trop Anim Health Prod.2012;44(1):17-20. [DOI:10.1007/s11250-011-9899-9] [PMID]

19. Dahl MO. Brucellosis in food-producing animals in Mosul, Iraq: A systematic review and meta-analysis. PLoS One. 2020;15(7):e0235862-e0235862. [DOI:10.1371/journal.pone.0235862] [PMID] [PMCID]

20. Jansen W, Linard C, Noll M, Nöckler K, Al Dahouk S. Brucella-positive raw milk cheese sold on the inner European market: A public health threat due to illegal import? Food Control. 2019; 100:130-7. [DOI:10.1016/j.foodcont.2019.01.022]

21. Authority EFS, Control EC for DP and. The European :union: summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2016. EFSA. 2017 ;15(12):e05077. [DOI:10.2903/j.efsa.2017.5077] [PMID]