Antibiotic Susceptibility Pattern and Distribution of Virulence Factors Among Klebsiella pneumoniae Isolated from Healthy Volunteers

Dalir, Amine; Razavi, Shabnam; Talebi, Malihe; Masjedian Jazi, Faramarz; Zahedi Bialvaei, Abed; Mirshekar, Maryam; Lohrasbi, Vahid

doi:10.30699/ijmm.15.6.676

year 15, Issue 6 (November - December 2021) Iran J Med Microbiol 2021, 15(6): 676-683 | Back to browse issues page

‎ 10.30699/ijmm.15.6.676

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Dalir A, Razavi S, Talebi M, Masjedian Jazi F, Zahedi Bialvaei A, Mirshekar M et al . Antibiotic Susceptibility Pattern and Distribution of Virulence Factors Among Klebsiella pneumoniae Isolated from Healthy Volunteers. Iran J Med Microbiol 2021; 15 (6) :676-683
URL: http://ijmm.ir/article-1-1482-en.html

Antibiotic Susceptibility Pattern and Distribution of Virulence Factors Among Klebsiella pneumoniae Isolated from Healthy Volunteers

Amine Dalir¹

, Shabnam Razavi

², Malihe Talebi¹

, Faramarz Masjedian Jazi¹

, Abed Zahedi Bialvaei³

, Maryam Mirshekar¹

, Vahid Lohrasbi¹

1- Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
2- Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran , razavi.sh@iums.ac.ir
3- Microbial Biotechnology Research Center, Iran University of Medical Sciences, Tehran, Iran

Abstract: (4597 Views)

Background and Objective: The healthy people’s fecal flora in the community represents a large potential reservoir. Therefore, the current study aimed to detect antibiotic resistance patterns and virulence factors in Klebsiella pneumoniae isolated from healthy volunteers’ feces.
Materials and Methods: Three hundred and fifty stool specimens were collected from sales rep healthy individuals referring to the Northwest Tehran Health Centers to get a health card. Bacterial isolation, identification, and antimicrobial susceptibility testing were conducted according to the routine instructions. In addition, polymerase chain reaction (PCR) was used to detect the genetic factors responsible for producing extended-spectrum β-lactamases (ESBLs: SHV, TEM, and CTX-M) blaKPC and other virulence genes.
Results: Among fecal samples analyzed, 60 (17.1%) K. pneumoniae were isolated. The results demonstrated that the highest resistance rate was related to piperacillin-tazobactam (n=25, 41.6%), followed by and meropenem (n=17, 28.8%) and co-trimoxazole (n=11, 18.3%), respectively. Also, all strains were susceptible to amikacin, gentamicin, and imipenem. The PCR results of the virulence gene showed that 95% (n=57) of isolates were positive for fimH gene, 93.33% (n=56) for BssS gene, 27 (45%) for rmpA gene. The PCR results for antibiotic resistance genes showed that 41.66% (n=25) had blaTEM gene, 38.33% (n=23) blaCTX-M gene, 35% (n=21) blaSHV gene and 3.33% (n=2) isolates had blaKPC gene, and none of these isolates carried magA gene.
Conclusion: Antibiotic resistance was common among K. pneumoniae isolated from healthy volunteers’ feces who participated in this study. Transmission of resistant bacteria and plasmids through oral-fecal sources threats to the public, which could complicate treatment options for community-acquired infections caused by K. pneumoniae.

Keywords: Antibiotic resistance, ESBL, Klebsiella pneumoniae, Virulence genes

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Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2021/09/12 | Accepted: 2021/11/10 | ePublished: 2021/12/8

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