year 2, Issue 1 (Spring 2008)                   Iran J Med Microbiol 2008, 2(1): 23-30 | Back to browse issues page

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Tavakkoli M, Soudi M, Malekzadeh F, Hajy Zarghany G. Effects of quinolones on biofilm formed by Staphylococcus epidermidisisolated from patients with urinary tract infection. Iran J Med Microbiol. 2008; 2 (1) :23-30
1- Islamic Azad university, Institute of Science and Research, Tehran , minoo
2- Department of Biological Science, School of Science, Alzahra university , Tehran
3- Department of microbiology, School of Biology , Pardis Olum , Tehran university , Tehran
4- Islamic Azad university, Institute of Science and Research, Tehran
Abstract:   (15278 Views)
Background and Objectives: Bacterial cells of Staphylococcus epidermidis are naturally occurring on skin and human mucosal membranes. They also cause nosocomial infections. Capability of biofilm formation plays an important role in the bacterial virulence. Quinolones have been used to treat urinary tract infections caused by S. epidermidis for several years. Thus, resistance to this type of antibiotics has emerged among the strains of this organism. Since the bacterial cells residing within biofilm structures are more resistant than those in planctonic stage, we conduct this study to examine the effect of quinolnes was the main goal of ones study of higher resistance of native biofilm producing strains is the goal of this project.
Material and Methods: In this research ten native isolates of S. epidermidis were obtained from pathients with urinary tract infection. Also standard strain of S. epidermidis PTCC 1435 was used as a control. Identification of strains was confirmed using morphological and biochemical tests. Challenge tests against the isolated was performed using three quinolone antibiotics including Ciprofluxacin, Ofluxacin, Nalidixic acid, with two different procedures :kirby bauer disk diffusion test, and broth dilution test.
Results: Average of MICs of above mentioned antibiotics against ten isolated was obtained as follow: Ciprofluxacin (7/375 µg/µl), Ofluxacin (11/53 µg/µl), Nalidixic acid (259/ 2 µg/µl). Experimental biofilm model of these bacteria showed much higher resistance to quinolone antibiotic, from 15 times in case of Nalidixic acid to 18 times greater resistance in case of Ciprofluxacin. Average of MICs amang ten isolates against the three antibiotics also showed increased resistance as follow: Ciprofluxacin ( 128/4 µg/µl ), Ofluxacin (177/8 µg/µl ), Nalidixic acid (3942 /4 µg/µl ).
Conclusion: S. epidermidis showed increased resistance to different quinolone antibiotics in biofilm structure, comparing to those of planktonic form. Results obtained from this research is in agreement with those of other similar projects and emphesize on applying of a de quaite doses of antibiotics against urinary infection caused by biofilmic Staphylococcus epidermidis.
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Type of Study: Original | Subject: Antimicrobial Substances
Received: 2013/11/14 | Accepted: 2013/11/14

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