year 2, Issue 1 (Spring 2008)                   Iran J Med Microbiol 2008, 2(1): 9-14 | Back to browse issues page

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Menbari S, Pourmand M, Shirazi M H, Mardani N. Cloning and Sequencing ofsacol a novel gene from Staphylococcus aureus . Iran J Med Microbiol. 2008; 2 (1) :9-14
1- Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran
2- Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran ,
Abstract:   (27399 Views)
Background & Objectives: Natural staphylococcal infections and vaccines based whole bacteria lead to poor antibody responses, but recent research reveals that specific antibodies based on recombinant staphylococcal antigens are much more protective. Sacol is a novel antigen that its structural and immunological traits poorly characterized. This research aimed to clone of sacol, a novel gene from Staphylococcus aureus.
Material and Methods: The specific primers with suitable restriction sites were designed and sacol amplified by PCR. The sacoland plasmid were produced as sticky ends by restriction enzymes NdeI and XhoI. To amplify the recombinant plasmid the pET21sacol transferred into competent cell E.coli TOP10. The recombinant plasmid harvested from the host and analyzed by restriction enzymes and sequencing. Finally, sacolgene analyzed by bioinformatics tools.
Results: The sacolgene has 723bp which amplified, cloned and sequenced successfully. Sacol is highly conserved in Staphylococcus aureus strains. Moreover, software analysis shows that sacolencodes a protein with 32KDa molecular weight (267 amino acids) which has similarity with C51 peptidase in N-terminal with one alpha helix and 14 beta sheets.
Conclusion: the sacolgene is conserved in majority of Staphylococcus aureus strains and may exist and express in most of staphylococcal infections.The role and regulation of the gene is thus of great interest.
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Type of Study: Original | Subject: Microbial Biotechnology
Received: 2013/11/14 | Accepted: 2013/11/14

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