Evaluation of the Effect of a Number of Commercial Disinfectants on Pseudomonas aeruginosa Isolates Obtained from Human Infection Cases

Hoseinpoor Mohammad Abadi, Zohre; Ebrahimi Kahrizsangi, Azizolah; Mokhtari, Azam

doi:10.30699/ijmm.14.2.138

year 14, Issue 2 (March - April 2020) Iran J Med Microbiol 2020, 14(2): 138-153 | Back to browse issues page

‎ 10.30699/ijmm.14.2.138

Mendeley

Zotero

RefWorks

Hoseinpoor Mohammad Abadi Z, Ebrahimi Kahrizsangi A, Mokhtari A. Evaluation of the Effect of a Number of Commercial Disinfectants on Pseudomonas aeruginosa Isolates Obtained from Human Infection Cases. Iran J Med Microbiol 2020; 14 (2) :138-153
URL: http://ijmm.ir/article-1-1016-en.html

Evaluation of the Effect of a Number of Commercial Disinfectants on Pseudomonas aeruginosa Isolates Obtained from Human Infection Cases

Zohre Hoseinpoor Mohammad Abadi

¹, Azizolah Ebrahimi Kahrizsangi²

, Azam Mokhtari²

1- Department of Pathobiology, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran , hoseinpoor325@gmail.com
2- Department of Pathobiology, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran

Abstract: (4300 Views)

Background: Disinfectants have important clinical applications. Antibiotic resistance of some hospital pathogens such as Pseudomonas aeruginosa has led to the use of disinfectants. In the present study, the effects of four commercial disinfectants; Hydrocare, Benzalkonium Chloride, Cetrimide-C, and Vico sience on Pseudomonas aeruginosa isolates were investigated.

Materials & Methods: The dilutions of four tested disinfectants recommended by the manufacturer were prepared and their effects on twelve Pseudomonas aeruginosa isolates were evaluated at five, ten, fifteen minutes after the bacterial inoculation.

Results & Discussion: Hydrocare and Benzalkonium Chloride inhibited the growth of all strains while Cetrimide-C disinfectant did not inhibit the growth of bacterial strains at any time. The Vico sience completely inhibited the growth of bacterial strains at ten and fifteen minutes after the addition of disinfectant. Since the recommended dilutions by the manufacturer were prepared and evaluated in the present study, the inefficiency of some of the disinfectants indicated that the evaluation and dilution assay of the disinfectant is necessary prior to routine use in laboratories or health centers.

Keywords: Disinfectant, Hydrocare, Benzalkonium Chloride, Vico sience, Cetrimide- C, Pseudomonas aeruginosa

Full-Text [PDF 1857 kb] (2132 Downloads) | | Full-Text (HTML) (2518 Views)

Type of Study: Original Research Article | Subject: Antimicrobial Substances
Received: 2019/12/23 | Accepted: 2020/05/4 | ePublished: 2020/03/9

References

1. Montagna MT, Triggiano F, Barbuti G, Bartolomeo N, De Giglio O, Diella G, Lopuzzo M, Rutigliano S, Serio G, Caggiano G. Study on the In Vitro Activity of Five Disinfectants against Nosocomial Bacteria. International journal of environmental research and public health. 2019 Jan; 16(11):1895. [DOI:10.3390/ijerph16111895] [PMID] [PMCID]

2. West AM, Teska PJ, Lineback CB, Oliver HF. Strain, disinfectant, concentration, and contact time quantitatively impact disinfectant efficacy. Antimicrobial Resistance & Infection Control. 2018 Dec; 7(1):49. [DOI:10.1186/s13756-018-0340-2] [PMID] [PMCID]

3. Xia J, Gao J, Tang W. Nosocomial infection and its molecular mechanisms of antibiotic resistance. Bioscience trends. 2016; 10(1):14-21. [DOI:10.5582/bst.2016.01020] [PMID]

4. Watanabe S, Ohnishi T, Yuasa A, Kiyota H, Iwata S, Kaku M, Watanabe A, Sato J, Hanaki H, Manabe M, Suzuki T. The first nationwide surveillance of antibacterial susceptibility patterns of pathogens isolated from skin and soft-tissue infections in dermatology departments in Japan. Journal of Infection and Chemotherapy. 2017 Aug 1; 23(8):503-11. [DOI:10.1016/j.jiac.2017.05.006] [PMID]

5. Yamagishi Y, Hagihara M, Kato H, Hirai J, Nishiyama N, Koizumi Y, Sakanashi D, Suematsu H, Nakai H, Mikamo H. In vitro and in vivo Pharmacodynamics of Colistin and Aztreonam Alone and in Combination against Multidrug-Resistant Pseudomonas aeruginosa. Chemotherapy. 2017; 62(2):105-10. [DOI:10.1159/000449367] [PMID]

6. Scano A, Serafi G, Fais S, Bomboi S, Peri M, Ibba A, Girometta G, Orrù G, Rossi P, Sanna P, Coghe F. Antimicrobial susceptibility pattern to disinfectants in Pseudomonas aeruginosa strains isolated from dairy sheep breeds in Sardinia. Large Animal Review. 2019 Apr 18; 25(1):11-5.

7. Stefani S, Campana S, Cariani L, Carnovale V, Colombo C, Lleo MM, Iula VD, Minicucci L, Morelli P, Pizzamiglio G, Taccetti G. Relevance of multidrug-resistant Pseudomonas aeruginosa infections in cystic fibrosis. International Journal of Medical Microbiology. 2017 Sep 1; 307(6):353-62. [DOI:10.1016/j.ijmm.2017.07.004] [PMID]

8. Ferris RA, McCue PM, Borlee GI, Glapa KE, Martin KH, Mangalea MR, Hennet ML, Wolfe LM, Broeckling CD, Borlee BR. Model of chronic equine endometritis involving a Pseudomonas aeruginosa biofilm. Infection and immunity. 2017 Dec 1; 85(12):e00332-17. [DOI:10.1128/IAI.00332-17] [PMID] [PMCID]

9. Argyraki A, Markvart M, Nielsen A, Bjarnsholt T, Bjørndal L, Petersen PM. Comparison of UVB and UVC irradiation disinfection efficacies on Pseudomonas Aeruginosa (P. aeruginosa) biofilm. InBiophotonics: Photonic Solutions for Better Health Care V 2016 Apr 27 (Vol. 9887, p. 988730). International Society for Optics and Photonics. [DOI:10.1117/12.2225597]

10. Lineback CB, Nkemngong CA, Wu ST, Li X, Teska PJ, Oliver HF. Hydrogen peroxide and sodium hypochlorite disinfectants are more effective against Staphylococcus aureus and Pseudomonas aeruginosa biofilms than quaternary ammonium compounds. Antimicrobial Resistance & Infection Control. 2018 Dec 1; 7(1):154. [DOI:10.1186/s13756-018-0447-5] [PMID] [PMCID]

11. ECDC 2018: https://www.thelancet.com/journals/laninf/article/PIIS1473-3099 (18)30605-4/sec1 (accessed on 23 January 2019).

12. Rahimzadeh Torabi L, Doudi M, Golshani Z. The frequency of blaIMP and blaVIM carbapenemase genes in clinical Isolates of pseudomonas aeruginosa in Isfahan medical centers. medical journal of mashhad university of medical sciences. 2016; 59(3):139-47.

13. Rokoei F, Rezaei S, Karbasian M, Sadaee N, Rastegar Lari A. Comparison of the antibacterial activity of Handsept and Decosept . Iran J Med Microbiol. 2008; 1 (4) :51-57

14. Subedi D, Vijay AK, Willcox M. Study of Disinfectant Resistance Genes in Ocular Isolates of Pseudomonas aeruginosa. Antibiotics. 2018 Dec; 7(4):88. [DOI:10.3390/antibiotics7040088] [PMID] [PMCID]

15. Espinal MA, Laszlo A, Simonsen L, Boulahbal F, Kim SJ, Reniero A, Hoffner S, Rieder HL, Binkin N, Dye C, Williams R. Global trends in resistance to antituberculosis drugs. New England Journal of Medicine. 2001 Apr 26; 344(17):1294-303. [DOI:10.1056/NEJM200104263441706] [PMID]

16. Abdollahzadeh Fatemeh, Hakami Vala Mojdeh, Bagheri Bajestani Fatemeh, Bahar Mohammad Reza. Evaluation of ESBL Extended Beta Lactamase Production from Pseudomonas aeruginosa strains isolated from burn patients admitted in Shahid Motahari Hospital. Tehran Iran. 2011; 47-42: 1.

17. Damin Teb Rooz. Intra Hyvarocare.2019. Availablc at: www.daminteb.com

18. Gasparini R, Pozzi T, Magnelli R, Fatighenti D, Giotti E, Poliseno G, Pratelli M, Severini R, Bonanni P, De Feo L. Evaluation of in vitro efficacy of the disinfectant Virkon. European journal of epidemiology. 1995 Apr 1; 11(2):193-7. [DOI:10.1007/BF01719487] [PMID]

19. Furi L, Ciusa ML, Knight D, Di Lorenzo V, Tocci N, Cirasola D, Aragones L, Coelho JR, Freitas AT, Marchi E, Moce L. Evaluation of reduced susceptibility to quaternary ammonium compounds and bisbiguanides in clinical isolates and laboratory-generated mutants of Staphylococcus aureus. Antimicrobial agents and chemotherapy. 2013 Aug 1; 57(8):3488-97. [DOI:10.1128/AAC.00498-13] [PMID] [PMCID]

20. Alzubeidi YS, Udompijitkul P, Talukdar PK, Sarker MR. Inactivation of Clostridium perfringens spores adhered onto stainless steel surface by agents used in a clean-in-place procedure. International journal of food microbiology. 2018 Jul 20; 277:26-33. [DOI:10.1016/j.ijfoodmicro.2018.04.016] [PMID]

21. Webb HE, Brichta-Harhay DM, Brashears MM, Nightingale KK, Arthur TM, Bosilevac JM, Kalchayanand N, Schmidt JW, Wang R, Granier SA, Brown TR. Salmonella in peripheral lymph nodes of healthy cattle at slaughter. Frontiers in microbiology. 2017 Nov 9; 8:2214. [DOI:10.3389/fmicb.2017.02214] [PMID] [PMCID]

22. Uppal S, Bazzi A, Reynolds RK, Harris J, Pearlman MD, Campbell DA, Morgan DM. Chlorhexidine-alcohol compared with povidone-iodine for preoperative topical antisepsis for abdominal hysterectomy. Obstetrics & Gynecology. 2017 Aug 1; 130(2):319-27. [DOI:10.1097/AOG.0000000000002130] [PMID]

23. Koehler DA, Strevett KA, Papelis C, Kibbey TC. The impact of antibacterial handsoap constituents on the dynamics of triclosan dissolution from dry sand. Chemosphere. 2017 Nov 1; 186:251-6. [DOI:10.1016/j.chemosphere.2017.07.142] [PMID]

24. Olasehinde GI, Akinyanju JA, Ajayi AA. Comparative Antimicrobial Activity of Commercial Disinfectants with Naphtolics. Research Journal of Microbiology. 2008; 3(4):262-8. [DOI:10.3923/jm.2008.262.268]

25. El-Banna T, El-Aziz AA, Sonbol F, El-Ekhnawy E. Adaptation of Pseudomonas aeruginosa clinical isolates to benzalkonium chloride retards its growth and enhances biofilm production. Molecular biology reports. 2019 Jun 1; 46(3):3437-43. [DOI:10.1007/s11033-019-04806-7] [PMID]

26. Vijaya k, Aboody MS, Alfanaisan MK. Sandle T. In vitro susceptibility of multidrug resistant pseudomonas aeruginosa clinical isolates to common biocides. International Journal of Pharmaceutical Sciences and Research. 2016; 7(1): 110-116.

27. Houari A, Di Martino P. Effect of chlorhexidine and benzalkonium chloride on bacterial biofilm formation. Letters in applied microbiology. 2007 Dec; 45(6):652-6. [DOI:10.1111/j.1472-765X.2007.02249.x] [PMID]

28. Olasehinde GI, Akinyanju JA, Ajayi AA. Comparative Antimicrobial Activity of Commercial Disinfectants with Naphtolics. Research Journal of Microbiology. 2008; 3(4):262-8. [DOI:10.3923/jm.2008.262.268]

29. Carson LA, Favero MS, Bond WW, Petersen NJ. Factors affecting comparative resistance of naturally occurring and subcultured Pseudomonas aeruginosa to disinfectants. Appl. Environ. Microbiol. 1972 May 1; 23(5):863-9. [DOI:10.1128/AEM.23.5.863-869.1972] [PMID] [PMCID]

30. Lotfipour F, Nahaee M, Milani M et al. Evaluation of the effect of several common hospital disinfectant products on patients with pseudomonas aeruginosa isolated from patients. Journal of Medical Sciences, Tabriz University of Medical Sciences, 2008; 72-67.

31. Gasparini R, Pozzi T, Magnelli R, Fatighenti D, Giotti E, Poliseno G, Pratelli M, Severini R, Bonanni P, De Feo L. Evaluation of in vitro efficacy of the disinfectant Virkon. European journal of epidemiology. 1995 Apr 1; 11(2):193-7. [DOI:10.1007/BF01719487] [PMID]