year 13, Issue 3 (July - August 2019)                   Iran J Med Microbiol 2019, 13(3): 210-219 | Back to browse issues page

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1- Faculty of Chemistry, University of Kashan, Kashan, Iran
2- Department of Cell and Molecular Biology , Faculty of Chemistry, University of Kashan, Kashan, Iran ,
Abstract:   (6916 Views)
Background and Aims: Increasing drug resistance to antibiotics in most bacteria has led to the development of natural antimicrobial compounds. Peppermint (Mentha piperita L.) is one of the most widely used herbs that has many therapeutic effects. The purpose of this study is the evaluation of antimicrobial and antioxidant activity of essential oil of Mentha piperita L.
Materials and Methods: : In this study, after collecting and drying the leaves, essential oils of the plant was extracted using a solvent-free microwave extraction. The compounds of essential oil were identified by the GC-MS (Gas chromatography-mass spectrometry). The effect of antioxidant was determined by beta-carotene test and antimicrobial activity was carried using agar-dilution method also, the minimum inhibitory concentration (MIC) value was evaluated.
Results & Discussion: Menthol, Menthofuran, and 1S-Neomenthyl acetate were the major compounds of essential oil of Mentha piperita L. collected from Marivan, respectively; menthol is the main compound of essential oil. The essential oil was more effective on gram-positive bacteria (Staphylococcus epidermidis, Bacillus subtilis, Staphylococcus aureus) and gram negative (Shigella dysenteriae, Klebsiella pneumonia) also had a good antioxidant activity compared to the standard BHT (butylated hydroxytoluene).Due to its relatively good antimicrobial and antioxidant properties, it can be used in the pharmaceutical and food industries.
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Type of Study: Brief Original Article | Subject: Antimicrobial Substances
Received: 2018/07/9 | Accepted: 2019/08/14 | ePublished: 2019/11/22

1. Tsai ML, Wu CT, Lin TF, Lin WC, Huang YC, Yang CH. Chemical composition and biological properties of essential oils of two mint species. Trop J Pharm Res. 2013; 12(4 ):577-82. [DOI:10.4314/tjpr.v12i4.20]
2. Fatemi F, Dini S, Rezaei MB, Dadkhah A, Dabbagh R, Naij S. The effect of γ-irradiation on the chemical composition and antioxidant activities of peppermint essential oil and extract. J Essent Oil Res. 2014; 26(2):97-104. [DOI:10.1080/10412905.2013.871670]
3. Trevisan SCC, Menezes APP, Barbalho SM, Guiguer ÉL. Properties of mentha piperita: a brief review. World J Pharm Med Res. 2017; 3(1):309-13.
4. Hoffmann BG, Lunder LT. Flavonoids from Mentha piperita leaves. Planta Med. 1984; 50(4):361-369. [DOI:10.1055/s-2007-969736] [PMID]
5. Farnad N, Heidari R, Aslanipour B. Phenolic composition and comparison of antioxidant activity of alcoholic extracts of Peppermint (Mentha piperita). J Food Me:as char:act. 2010; 8(2):113-121. [DOI:10.1007/s11694-014-9171-x]
6. Rahmani F, Rezaeian-Doloei R, Alimoradi L. Evaluation of Phytochemical Composition of Mentha pulegium L. Essential Oil and Its Antibacterial Activity against Several Pathogenic Bacteria. Iran J Med Microbiol. 2018; 11 (6) :167-177
7. Clark GS. An aroma chemical profile. Menthol. Perfum Flavor. 1998; 23:33-46
8. Ernestt E, Pittler MH. The efficacy and safety peppermint (Mentha piperita L.): an update of a systemic review. Public Health Nutr. 2001; 3(4):509-14.
9. Adams R. Identification of essential oil components by gas chromatography/mass spectroscopy. J Am Soc Mass Spectrom. 1997; 6:671-82. [DOI:10.1016/S1044-0305(97)00026-3]
10. Moure A, Franco D, Sineiro J, Domínguez H, Núñez MJ, Lema JM. Evaluation of extracts from Gevuina avellana hulls as antioxidants. J Agric Food Chem. 2000; 48(9):3890-7. [DOI:10.1021/jf000048w] [PMID]
11. Watts JL, Clinical and Laboratory Standards Institute. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals: approved standard. National Committee for Clinical Laboratory Standards; 2008.
12. da Silva Ramos R, Rodrigues ABL, Farias ALF, Simões RC, Pinheiro MT, Ferreira RMdA, et al. Chemical Composition and In Vitro Antioxidant, Cytotoxic, Antimicrobial, and Larvicidal Activities of the Essential Oil of Mentha piperita L.(Lamiaceae). Sci World J. 2017; 2017:1-8. [DOI:10.1155/2017/4927214] [PMID] [PMCID]
13. Fadaei S, Aberoomand AP, Sharifan A, Larijani K. Comparison of Antibacterial Activity and Chemical Composition of Essential Oil Extracted from Mentha piperita L. Herb by Microwave and Hydrodistillation Methods. Food Technol Nutr. 2011; 8(3):28-36. (Persian)
14. Mimica-Dukić N, Božin B, Soković M, Mihajlović B, Matavulj M. Antimicrobial and antioxidant activities of three Mentha species essential oils. Planta med. 2003; 69(5):413-9. [DOI:10.1055/s-2003-39704] [PMID]
15. Izadi Z, Esna-Ashari M, Ahmadvand G, Davoodi P, Piri K. Chemical composition and antibacterial activity of the essence oil of peppermint (Mentha piperita L). Armaghane danesh. 2009; 14(3):45-54.
16. Soković MD, Vukojević J, Marin PD, Brkić DD, Vajs V, Van Griensven LJ. Chemical composition of essential oilsof thymus and mentha speciesand their antifungal activities. Molecules. 2009; 14(1):238-49. [DOI:10.3390/molecules14010238] [PMID] [PMCID]
17. Kanza S, Imran P, Hina B, Butt MS, Tayyaba I, Ushnah SU. Compositional profiling of Mentha piperita. Pakistan J Food Sci. 2014; 24(3):151-6.
18. Alankar S. A review on peppermint oil. Asian J Pharm Clin Res. 2009; 2(2):27-33.
19. Yadegarinia D, Gachkar L, Rezaei MB, Taghizadeh M, Astaneh SA, Rasooli I. Biochemical activities of Iranian Mentha piperita L. and Myrtus communis L. essential oils. Phytochemistry. 2006; 67(12):1249-55. [DOI:10.1016/j.phytochem.2006.04.025] [PMID]
20. Başer K, Kürkçüoğlu M, Demirci B, Özek T, Tarımcılar G. Essential oils of Mentha species from Marmara region of Turkey. J Essent Oil Res. 2012; 24(3):265-72. [DOI:10.1080/10412905.2012.676775]
21. İşcan G, Ki̇ri̇mer N, Kürkcüoǧlu Mn, Başer HC, DEMIrci F. Antimicrobial screening of Mentha piperita essential oils. J Agric Food Chem. 2002; 50(14):3943-6. [DOI:10.1021/jf011476k] [PMID]
22. Sivropoulou A, Papanikolaou E, Nikolaou C, Kokkini S, Lanaras T, Arsenakis M. Antimicrobial and cytotoxic activities of Origanum essential oils. J agric Food Chem. 1996; 44(5):1202-5. [DOI:10.1021/jf950540t]
23. Afridi MS, Ali J, Abbas S, Rehman SU, Khan FA, Khan MA, et al. Essential oil composition of Mentha piperita L. and its antimicrobial effects against common human pathogenic bacterial and fungal strains. Pharmacol Online. 2016; 3:90-7.
24. Gharenaghadeh S, Forghani S, Gharehnaghadeh S, Sowti M. Evaluation of Antimicrobial Properties of Methanolic Extract, Essential Oil and Nanolipozom of Mentha piperita. J Food Sci Technol. 2017; 14(68):93-102. (Persian)
25. Singh R, Shushni MA, Belkheir A. Antibacterial and antioxidant activities of Mentha piperita L. Arab J Chem. 2015; 8(3):322-8. [DOI:10.1016/j.arabjc.2011.01.019]
26. Tabatabaei Yazdi F, Alizadeh Behbahani B, Vasiee AR, Mortazavi SA, Shahidi F. Evaluation antioxidant activity, phytochemical constituents and antimicrobial of Mentha Piperita essential oil on some infectious and poisonous microorganisms. J Food Sci Technol. 2018; 15(76):67-76.

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