year 11, Issue 6 (January - February 2018)                   Iran J Med Microbiol 2018, 11(6): 184-191 | Back to browse issues page


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1- Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran
2- Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran ,
3- Department of Chemistry, Rasht Branch, Islamic Azad University, Rasht, Iran
Abstract:   (9817 Views)
Background and Aims: Drug resistance, particularly methicillin resistant in Staphylococcus aureus strain is a major worldwide public health concern. The present study aimed to synthetize selenium nanoparticles, investigate its antibacterial effect and its ability to be used as ampicillin nanocarrier.
Materials and Methods: Selenium nanoparticles were synthetized via chemical regeneration of sodium selenite by L-systein amino acid. Loading of ampicillin on the surface of selenium nanoparticles was done by gradual addition of antibiotic to nanoparticle solution and continuous shaking. Then this attachment was investigated by using UV-Vis spectroscopy, XRD and scanning electron microscope. Antibacterial properties of produced selenium nanoparticles and antibiotic loaded selenium nanoparticles against standard strain of S. aureus and 10 methicillin resistant S. aureus strains were tested by disc diffusion method. Minimum inhibitory concentration (MIC) of bacterial growth was determined by broth macrodilution method.
Results: The produced selenium nanoparticles showed antibacterial effect against all strains of S. aureus. Loading of ampicillin on the surface of selenium nanoparticles enhanced the antimicrobial activity of this drug. The mean MIC of selenium nanoparticles against S. aureus strains ranged between 7.8-62.5 µg/mL free form of ampicillin between 62.5- 250 and ampicillin bound to nanoparticles was 7.8-7.8 μg / mL. These values for standard strain of Staphylococcus aureus strains were 7.8, 31.2 and 3.9 μg / mL, respectively. Loading of ampicillin on the surface of selenium nanoparticles enhanced the MIC against methicillin resistant S. aureus.
Conclusions: The obtained results confirmed antibacterial activity of selenium nanoparticles. Enhancing the antibacterial activity of antibiotics loaded on the surface of nanoparticles, increases the possibility of application of these drugs especially for the elimination of hard-to-heal infective diseases.
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Type of Study: Original Research Article | Subject: Antimicrobial Substances
Received: 2017/08/7 | Accepted: 2018/01/14 | ePublished: 2018/03/19

1. Enright MC, Day NP, Davies CE, Peacock SJ, Spratt BG. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J Clin Microbiol. 2000;38(3):1008-15. [PubMed]
2. Huang YC, Su LH, Wu TL, Lin TY. Changing molecular epidemiology of methicillin-resistant Staphylococcus aureus bloodstream isolates from a teaching hospital in Northern Taiwan. J Clin Microbiol. 2006;44(6):2268-70. [DOI] [PubMed]
3. Luo J, Chan W-B, Wang L, Zhong C-J. Probing interfacial interactions of bacteria on metal nanoparticles and substrates with different surface properties. Int J Antimicrob Agents. 2010;36(6):549-56. [DOI] [PubMed]
4. Li W-R, Xie X-B, Shi Q-S, Zeng H-Y, You-Sheng O-Y, Chen Y-B. Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli. Appl Microbiol Biotechnol. 2010;85(4):1115-22. [DOI] [PubMed]
5. De Jong WH, Borm PJ. Drug delivery and nanoparticles: applications and hazards. Int J Nanomedicine. 2008;3(2):133-149. [DOI] [PubMed]
6. Wang Q, Webster TJ. Nanostructured selenium for preventing biofilm formation on polycarbonate medical devices. J Biomed Mater Res A. 2012;100(12):3205-10. [DOI] [PubMed]
7. Shakibaie M, Forootanfar H, Golkari Y, Mohammadi-Khorsand T, Shakibaie MR. Anti-biofilm activity of biogenic selenium nanoparticles and selenium dioxide against clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa, and Proteus mirabilis. J Trace Elem Med Biol. 2015;29:235-41. [DOI] [PubMed]
8. Yu B, Zhang Y, Zheng W, Fan C, Chen T. Positive surface charge enhances selective cellular uptake and anticancer efficacy of selenium nanoparticles. Inorg Chem. 2012;51(16):8956-63. [DOI] [PubMed]
9. Fesharaki PJ, Nazari P, Shakibaie M, Rezaie S, Banoee M, Abdollahi M, et al. Biosynthesis of selenium nanoparticles using Klebsiella pneumoniae and their recovery by a simple sterilization process. Braz J Microbiol. 2010;41(2):461-6. [DOI] [PubMed]
10. Wayne P. Clinical and Laboratory Standards Institute: Performance standards for antimicrobial susceptibility testing: Twenty-fourth informational supplement, M100-S24. Clinical and Laboratory Standards Institute (CLSI). 2014;34(1).
11. Tran PA, Webster TJ. Selenium nanoparticles inhibit Staphylococcus aureus growth. Int J Nanomedicine. 2011;6:1553-8. [PubMed]
12. Tran PA, O’Brien-Simpson N, Reynolds EC, Pantarat N, Biswas DP, O’Connor AJ. Low cytotoxic trace element selenium nanoparticles and their differential antimicrobial properties against S. aureus and E. coli. Nanotechnology. 2015;27(4):045101. [DOI] [PubMed]
13. Hariharan H, Al-Harbi N, Karuppiah P, Rajaram S. Microbial synthesis of selenium nanocomposite using Saccharomyces cerevisiae and its antimicrobial activity against pathogens causing nosocomial infection. Chalcogenide Lett. 2012;9(12):509-15.
14. Chudobova D, Cihalova K, Dostalova S, Ruttkay-Nedecky B, Merlos Rodrigo MA, Tmejova K, et al. Comparison of the effects of silver phosphate and selenium nanoparticles on Staphylococcus aureus growth reveals potential for selenium particles to prevent infection. FEMS Microbiol Lett. 2014;351(2):195-201. [DOI] [PubMed]
15. Wang Q, Webster TJ. inhibiting biofilm formation on paper towels through the use of selenium nanoparticles coatings. Int J Nanomedicine. 2013;8:407. [PubMed]
16. El-Batal A, Essam TM, El-Zahaby DA, Amin MA. Synthesis of Selenium Nanoparticles by Bacillus laterosporus Using Gamma Radiation. Br J Pharm Res. 2014;4(11):1364-86. [DOI]
17. Bhattacharya D, Saha B, Mukherjee A, Santra CR, Karmakar P. Gold nanoparticles conjugated antibiotics: stability and functional evaluation. J Nanosci Nanotechnol. 2012;2(2):14-21. [DOI]
18. Kora AJ, Rastogi L. Enhancement of antibacterial activity of capped silver nanoparticles in combination with antibiotics, on model gram-negative and gram-positive bacteria. Bioinorg Chem Appl. 2013;2013.
19. Brown AN, Smith K, Samuels TA, Lu J, Obare SO, Scott ME. Nanoparticles functionalized with ampicillin destroy multiple-antibiotic-resistant isolates of Pseudomonas aeruginosa and Enterobacter aerogenes and methicillin-resistant Staphylococcus aureus. Appl Environ Microbiol. 2012;78(8):2768-74. [DOI] [PubMed]
20. Hussein-Al-Ali SH, El Zowalaty ME, Hussein MZ, Geilich BM, Webster TJ. Synthesis, characterization, and antimicrobial activity of an ampicillin-conjugated magnetic nanoantibiotic for medical applications. Int J Nanomedicine. 2014;9:3801-14. [DOI] [PubMed]

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