year 17, Issue 2 (March - April 2023)
Iran J Med Microbiol 2023, 17(2): 161-166 |
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Negar Joharinia1 
, Seyed Ammar Mousavi-Nejad1 , Ali Farhadi2 , Akbar Safaei3 , Seyed Younes Hosseini1 , Jamal Sarvari 4
, Seyed Ammar Mousavi-Nejad1 , Ali Farhadi2 , Akbar Safaei3 , Seyed Younes Hosseini1 , Jamal Sarvari 4
1- Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2- Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
3- Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
4- Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran , sarvarij@sums.ac.ir
2- Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
3- Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
4- Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran , sarvarij@sums.ac.ir
Abstract: (1436 Views)
Background and Aim: Determination of HPV genotypes in cervical lesions is essential in screening programs and the impact of current HPV vaccines. Therefore, we investigated the frequency of various HPV genotypes among different grades of cervical lesions.
Materials and Methods: In total, 101 biopsy samples of different grades of cervical lesions were collected. Extracted DNA was subjected to PCR amplification of beta-globin to assess sample integrity. Duplex Taq-Man Real-Time PCR was performed to identify HPV types 16 and 18. Nested PCR following sequencing was done on those samples with HPV16/18 negative results.
Results: The mean age of participants was 43±1. Of 101 samples, 91 (90%) were infected with HPVs. In the LSIL group, HPV genotypes 16, 18, and 51; in the HSIL group, genotypes 6, 16, 18, 31, and 53; in the SCC group, genotypes 6, 16, 18, and 3; and in the AD group, HPV genotypes 16 and 18 were detected. Also, 49 (61%) samples had co-infection with HPV16 and HPV18.
Conclusion: Our results showed that HPV 16 and 18 were the most prevalent genotypes among different grades of lesions. The presence of low-risk HPV types, including genotypes 6 and 53, might need more attention in HPV screening and prevention programs.
Type of Study: Original Research Article |
Subject:
Medical Virology
Received: 2022/05/5 | Accepted: 2022/10/17 | ePublished: 2023/03/30
Received: 2022/05/5 | Accepted: 2022/10/17 | ePublished: 2023/03/30
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