Ghasemi A, Shirazi M, Pourmand M, Zaemi Yazdi J, Sadeghifard N, Bagherzadeh S et al . Genotyping of Helicobacter pyloristrains isolated from patients with NUD, DU, GU and GC by RAPD-PCR. Iran J Med Microbiol 2007; 1 (2) :21-25
URL:
http://ijmm.ir/article-1-81-en.html
1- Department of Pathobiology, School of Public Health, Medical Sciences/University of Tehran
2- Department of Pathobiology, School of Public Health, Medical Sciences/University of Tehran , mpourmand@tums.ac.ir
Abstract: (14711 Views)
Background and Objectives: Helicobacter pylori is a genetically diverse gastric pathogen that chronically
infects billions of people worldwide, typically beginning in infancy and lasting for decades. It is a major
cause of peptic ulcers and it is an early risk factor for gastric cancer which is the most frequently lethal
malignancy globally. This project was designed to genotype H. pyloriisolates isolated from patients with
NUD, DU, GU and GC by the polymerase chain reaction(PCR)-based on Randomly Amplified Polymorphic
DNA (RAPD) fingerprinting technique.
Material and Methods: Eighty patients admitted to the gastroenterology unit at Sharyati hospital in Iran
were included in this study. Gastric biopsy specimens were inoculated onto selective medium then were
cultured for 3 to 5 days at 37
°
C under microaerobic conditions. Genomic DNA was extracted using a
commercially available Qiagen kit. RAPD-PCR was used to genotype isolates.
Results: Six different RAPD patterns (A-F) were seen in morethan one isolate which were as follow pattern
A: 9 (16.98%), B: 6 (11.33%), C: 5 (9.43%), D: 3 (5.66%), E: 2 (3.77%) and F: 2 (3.77%). Twenty six
(49.06%) of 53 isolates showed a unique RAPD pattern that were not similar to each other. A significant
relationship was not seen between a single RAPD pattern and a gastric disorder (P>0.05).
Conclusion: The results of this study suggest a high level of DNA sequence diversity among H. pylori
isolates and it is better to use sequencing method for surveying of Helicobacter pylorigenome rather than
RAPD-PCR.
Keywords: H
Type of Study:
Brief Original Article |
Subject:
Molecular Microbiology Received: 2013/11/10 | Accepted: 2013/11/11 | ePublished: 2013/11/11