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:: Volume 11, Issue 4 (September - October 2017) ::
Iran J Med Microbiol 2017, 11(4): 1-12 Back to browse issues page
Quorum-Sensing Signaling System in Pseudomonas aeruginosa: A Method to Overcome the Bacteria’s antibioticResistance By Identifying Dual Inhibitors and Using NSAIDs in Combination with Antibiotic Against Biofilm
Mohammad Besharati 1, Ali ZhianiAsgharzadeh 2, Vahid Soheili 3, Bibi Sedigheh Fazli Bazzaz 3, Majid Rajabian Noghondar 4
1- Department of Biochemistry, Payam Noor University of Mashhad, Mashhad, Iran , besharat61@yahoo.com
2- Student Research Committee, Golestan University of Medical Sciences, Gorgan, Iran
3- Department of Food and Drug Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
4- Department of Biochemistry, Payam Noor University of Mashhad, Mashhad, Iran
Abstract:   (647 Views)

Background and Aims: Nonsteroidal anti-inflammatory drugs (NSAIDs), are used as analgesic and antipyretic agents. In this study NSAIDs were used to overcome the resistance of Pseudomonas aeruginosa biofilms to Tobramycin antibiotics. The aim of this study was to find an easy and low-cost method, using NSAIDs and Tobramycin, to deal with the biofilms of P. aeruginosa..
Materials and Methods: Follwoing molecular remodeling, NSAIDs were used alongside Tobramycin against PAO1 strain of P. aeroginusa . The Plate Reader measured the absorbance of crystal violet solution in ethanol. The Infiltration rate of NSAIDs in biofilms and its absorbance were measured by Tetrazolium salt and Plate Reader, respectively.
Results: After performing the docking techniques, the most suitable conformation of NSAIDs, to inhibit LasR and PqsE proteins, were chosen. Having worked on dual inhibitors, we could find a configuration  in which the inhibition coefficient was at its lowest level for both PqsE and LasR.
After analyzing the growth of P. aeruginosa biofilms near NSAIDs by using Tobramycin and measuring the absorption of crystal violet in ethanol, as a measure of composed biofilm, The findings showed a lower rate of biofilm formation by bacteria treated with NSAIDS and Tombramycin. [P1] 
Conclusions: Based on the constructional similarities in some NSAIDs, like Piroxicam, Meloxicam and Homoserine lactones (AHLS), these drugs can be used as quorum-sensing system inhibitors to inhibit LasR and PqsE protein and hence reduce the P. aeruginosa pathogenesis.

 [P1]Not mentioned in the abstract in Persian
Keywords: Pseudomonas aeruginosa, Biofilm, Tobramycin, Docking, Quorum sensing, NSAIDs
Full-Text [PDF 857 kb]   (217 Downloads)    
Type of Study: Original | Subject: Medical Bacteriology
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Besharati M, ZhianiAsgharzadeh A, Soheili V, Fazli Bazzaz B S, Rajabian Noghondar M. Quorum-Sensing Signaling System in Pseudomonas aeruginosa: A Method to Overcome the Bacteria’s antibioticResistance By Identifying Dual Inhibitors and Using NSAIDs in Combination with Antibiotic Against Biofilm. Iran J Med Microbiol. 2017; 11 (4) :1-12
URL: http://ijmm.ir/article-1-692-en.html

Volume 11, Issue 4 (September - October 2017) Back to browse issues page
مجله میکروب شناسی پزشکی ایران Iranian Journal of Medical Microbiology
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