Evaluation of indirect fluorescent antibody assay for detection of Nocardia

Eshraghi, Seyed Saeid; Sarrafnejad, Abdolfatah; Mazdeh, Shahnaz; Assasi, Nazila

year 1, Issue 1 (Spring 2007) Iran J Med Microbiol 2007, 1(1): 1-9 | Back to browse issues page

PMCID: 27863040

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Eshraghi S S, Sarrafnejad A, Mazdeh S, Assasi N. Evaluation of indirect fluorescent antibody assay for detection of Nocardia. Iran J Med Microbiol 2007; 1 (1) :1-9
URL: http://ijmm.ir/article-1-67-en.html

Evaluation of indirect fluorescent antibody assay for detection of Nocardia

Seyed Saeid Eshraghi

¹, Abdolfatah Sarrafnejad²

, Shahnaz Mazdeh³

, Nazila Assasi⁴

1- Department of Pathobiology, School of Public Health, Medical Sciences University of Tehran , eshraghs@sina.tums.ac.ir
2- Department of Pathobiology, School of Public Health, Medical Sciences University of Tehran
3- Imam Khomeini Teaching hospital
4- Department of Statistic, School of Public Health, Medical Sciences University of Tehran

Abstract: (20539 Views)

Background and Objectives: Nocardiosis is an acute or suppurative chronic disease caused by an aerobic, gram-positive, weakly acid-fast and soil-borne filamentous and organism. Nocardia asteroides which is the dangerous and most frequently pathogen, infects humans through the respiratory tract. The bacterium is primarily an opportunistic pathogen that causes the infection in patients with underlying immunodeficiencies. The main purpose of the study was to detect antibody titre against Nocardiain all study groups, using indirect immunofluorescent assay (IFA). Correlation between the antibody titre against Nocardiawith age, sex, occupation, and chronic pulmonary infection and corticosteroid therapy patients was also investigated.
Material and Methods: The present investigation is a Cross–Sectionalstudy conducted on a population consisted of 300 subjects including 200 hospitalized individuals' patients, nurses and healthcare workers from Imam Khomeini hospital, and 100 health adult blood donors. None of the patients had already been diagnosed to be affected by Nocardia.
Results: Our results demonstrated four patients suffering from different infections, including TB, mycetoma, chronic pulmonary and chronic obstructive pulmonary diseases were IFA positive. None of the high risk hospital personnel, who were working in close proximity to the areas infected with Nocardia, were found to be IFA positive. Meanwhile there was no positive result in a group of patients (n=34) who were under corticosteroid therapy.
Conclusion: Finally, considering the small sample size of the IFA positive cases no significant association between the IFA results and age, sex, occupation and clinical conditions of the subjects could be established.

Keywords: Nocardiosis, Nocardia asteroides, immunodeficiencies, Indirect immunofluorescence assay (IFA), Chronic obstructive pulmonary disease (COPD)

Full-Text [PDF 223 kb] (2596 Downloads)

Type of Study: Original Research Article | Subject: Medical Mycology
Received: 2013/11/10 | Accepted: 2013/11/10 | ePublished: 2013/11/10

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