year 10, Issue 6 (January - February 2017)                   Iran J Med Microbiol 2017, 10(6): 34-43 | Back to browse issues page

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Arbab Soleimani N, Kasra Kermanshahi R, Yakhchali B. The Effect of Prebiotic Chitosan on the growth and antimicrobial characteristic of Probiotic lactobacilli. Iran J Med Microbiol. 2017; 10 (6) :34-43
URL: http://ijmm.ir/article-1-600-en.html
1- Department of Microbiology, Damghan Branch, Islamic Azad University, Damghan, Iran , nazilaarbab@yahoo.co.uk
2- Department of Biology, Alzahra University, Tehran, Iran
3- National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
Abstract:   (5409 Views)

Background and Aim: Nowadays, prebiotics are the matter of interest, because of stimulating the growth and activity of beneficial enteric bacteria (probiotic) and their antimicrobial and antitumor characteristics. The aim of this research is to study the effect of prebiotic Chitosan on the growth of probiotic bacteria and their antimicrobial effect.

Materials and Methods: This research was done in 2010 in order to study of the effect of prebiotic chitosan on the growth of probiotic bacteria Lactobacillus acidophilus PTCC 1643 and Lactobacillus casei PTCC 1608 by using of drawing growth curve of these bacteria in the presence of different chitosan concentrations, also, the effect of this prebiotic on antimicrobial properties of probiotic bacteria was investigated against Escherichia coli by using of overlay method and blank disk method.

Results: According to the test results, the growth of two probiotic bacteria were increased in the presence of chitosan and the most effective concentration of prebiotic chitosan was achieved, 6.5 (mg/mL).Antimicrobial effects of probiotic bacteria were increased in the presence of chitosan against E.coli especially Entrohaemoragic E. coli in comparison with the time that probiotic bacteria were used alone.

Conclusions: According to the results of this study, prebiotic chitosan, due to increasing effect on the growth and antimicrobial characteristic of probiotic lactobacillus, can be a proper candidate for effective symbiotic compound against pathogenic bacteria.

Full-Text [PDF 590 kb]   (1328 Downloads)    
Type of Study: Original | Subject: Antimicrobial Substances
Received: 2016/07/31 | Accepted: 2017/01/14

References
1. Cardelle CA, Corzo N, Olano A, Peláez C, Requena T,Ávila M. Galactooligosaccharides derived from lactose and lactulose: Influence of structure on Lactobacillus, Streptococcus and Bifidobacterium growth. Int J Food Microbiol 2011; 149: 81-87. [PubMed]
2. Gibson GR, Rastall RA. Prebiotics: Development & Application. John Wiley & Sons Ltd; 2006. [Article]
3. Kolida S, Tuohy K, Gibson GR. Prebiotic effects of inulin and oligofructose. British J of Nut 2002;87)2): 193–197 [PubMed]
4. Rastall RA. Functional Oligosaccharides: Application and Manufacture. Annu Rev Food Sci Tech 2010; 1: 305-339 [PubMed]
5. Oliver L, Rasmussen H, Gregoire MB, Chen Y. Health care provider's knowledge, perceptions, and use of probiotics and prebiotics. Top Clin Nutr 2014; 29: 139–149 [Article]
6. Wlodzimierz G, Olejnik A, Sip A. Probiotics, prebiotics and antioxidants as functional foods.Acta Biochimica polonica 2005; 52)3( 665–671 [PubMed]
7. Martinez RCR, Aynaou AE, Albrecht S, Schols HA, Martinis ECP, Zoetendal EG, Venema K, Saad SMI, Smidt H. In vitro evaluation of gastrointestinal survival of Lactobacillus amylovorus DSM 16698 alone and combined with galactooligosaccharides, milk and/or Bifidobacterium animalis subsp. lactis Bb-12. Int J Food Microbiol 2011; 149: 152-158 [PubMed]
8. Verse M, Marteau R P. Probiotics and Prebiotics: Effects on Diarrhea. The J of Nut 2007;137: 803–811. [Article]
9. Holscher J, Caporaso G, Hooda S, Brulc JM, Fahey GC, Swanson KS. Fiber supplementation influences phylogenetic structure and functional capacity of the human intestinal microbiome: follow-up of a randomized controlled trial. Am J Clin Nutr 2015; 101: 55–64. [PubMed]
10. Kong M, Chen XG, Xing K, Park HJ. Antimicrobial properties of chitosan and mode of action: a state of the art review. Inter. J. Food Microb 2010; 144: 51-63. [PubMed]
11. Simunek J, Tishchenko G, Hadrova B. Effect of chitosan on the growth of human colonic bacteria.Folia Microbiol 2006; 51 (4):306-308. [PubMed]
12. Tang H, Zhang P, Kieft T, Ryon S, Baker S, Wiesmann W, Rogelji S. Antibacterial action of anoval functionalized chitosan arginine against Gram- bacteria. Acta Biomaterialia 2010; 6: 2562-2571. [PubMed]
13. Rhoades J, Rastall Bob. Chitosan as an antimicrobial agent. Food Tech Int 2002; 31-33.
14. Brink M, Todoro SD, Martin JH, Senekal M, Dicks LMT. The effect of prebiotics on production of antimicrobial compounds, resistance to growth at low pH and in the presence of bile, and adhesion of probiotic cells to intestinal mucus. J of Microbiol 2006; 100: 813–821. [PubMed]
15. Maia OB, Duarte R, Silva AM. Evaluation of the components of a commercial probiotic in gnotobiotic mice experimentally challenged with Salmonella entrica subsp.entrica ser. Typhimurium. Vet. Microbiol 2001; 79: 183-189. [PubMed]
16. Jacobsen CN. Screening of probiotic activities of forty-seven strain lactobacillus ssp.by invitro techniques and evaluation of the colonization ability of five selected strains in umans. Appl. Environ. Microbial 1999; 65: 4949-4956. [Article]
17. Costalos C, Kapiki A, Apostolou, M, Papathoma E. The effect of a prebiotic supplemented formula on growth and sool microbiology of term infants. Early Hum Dev 2007; 84: 45–49. [PubMed]
18. Caplan MS. Probiotic and prebiotic supplementation for the prevention of neonatal necrotizing enterocolitis. J of Perinatology 2009; 29: 2–6. [PubMed]
19. Martens EC, Kelly AG, Tauzin AS, Brumer H. The devil lies in the details: how variations in polysaccharide fine-structure impact the physiology and evolution of gut microbes. J Mol Biol. 2014; 426: 3851–3865. [PubMed]
20. Ping Su, Henriksson A, HazelMitchel l.Selected prebiotic support the growth of probiotic monocultures in vitro.Anaerob 2007; 13(3): 134-139. [PubMed]
21. Woo LH, Park YS, Jung JS, Shin WS.Chitosan oligosaccharides, dp2–8, have prebiotic effect on the Bifidobacterium bifidium and Lactobacillus sp. Anaerobe 2003; 8(6): 319-324. [PubMed]
22. Bindels LB, Delzenne NM, Cani PD, Walter J. Towards a more comprehensive concept for prebiotics. Nat Rev Gastroenterol Hepatol 2015; 12: 303–310. [PubMed]
23. Asahara T, Nomoto K, Watanuki M. Antimicrobial Activity of Intraurethrally Administered Probiotic Lactobacillus casei in a Murine Model of Escherichia coli Urinary Tract Infection. Antimicro agents chemother 2001; 45(6): 1751–1760. [PubMed]
24. Heller KJ. Probiotic bacteria in fermented foods: product characteristics and starter organisms. Am J Clin Nutr 2001; 73: 374–379. [PubMed]
25. Fooks LJ, Gibson GR. In vitro investigations of the effect of probiotics and prebiotics on selected human intestinal pathogens. FEMS Microbiol Ecol 2002; 39: 67–75. [PubMed]
26. Kasra Kermanshahi R, Goudarzi L.T he Effect of Prebiotics on Production of Antimicrobial Compounds from Lactobacillus spp. Against Proteus mirabilis (ATCC 7002 and PTCC 1076). Iranian Food Sci Technol Res J 2015; 11 (1): 41-47. [in Persian]
27. Jehan A, Salman S. Synbiotic Effect of Probiotic (Bifidobacterium sp.) and Prebiotics (Chicory and Inulin) aganist some pathogenic bacteria. Um-Salama Sci J 2009; 6(2): 354-360. [Article]
28. Ravi D, Usha G, Parthasarathy R.Synthesis of Bacteriocin by Synbiotic Effect and Its Antibacterial Activity against Selected Respiratory tract Pathogens. Int J of Adv Res 2013; 1(10) :296-303. [Article]

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