year 10, Issue 4 (September - October 2016)                   Iran J Med Microbiol 2016, 10(4): 25-33 | Back to browse issues page

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Majdani R. A study on antibacterial effect of nanosilver colloidal solution against Escherichia coli isolates from human and poultry in North-West of Iran. Iran J Med Microbiol. 2016; 10 (4) :25-33
Department of Biology, Faculty of Science, University of Maragheh, Maragheh, Iran ,
Abstract:   (6272 Views)

Background and Aim: Because of emerging resistant bacterial strains to antimicrobial agents, using effective alternatives such as silver nanoparticles has become unavoidable.

Materials and Methods: For the first time,  300 E.coli were isolated from humans, healthy poultry and poultry that their sensitivity were evaluated  to the four different concentration of solution of colloidal silver nanoparticles (40, 80, 400 and 4000 ppm) with the size of 5 and 20 nm using standard disk diffusion method in the North-West of Iran. Then statistical analysis of the results was performed

Results: The largest zone of inhibition (ZOI) was related to suspected isolates of colibacillosis in most concentrations of both solution of silver nanoparticles. By comparing the results of different used concentrations in three groups, except the solution of 20 nm which was suspected to Colibacillosis and isolated from poultry, there was no significant difference between concentrations of 40 and 80 ppm. By using 5 nm colloidal solution, the statistically significant difference was observed in all concentration among the mean zone of inhibition in three isolated groups. Whereas, the significant difference was not shown in of 40 and 80 concentrations (ppm) among three groups isolates.

Conclusions: For effective use of antibacterial properties of single nanoparticles colloidal solutions, having enough knowledge about regional strains, their susceptibility and properties of the silver nanoparticles are inevitable.Application of colloidal solutions of silver nanoparticles as an effective antibacterial agents against E. coli isolates needs enough information about regional isolates and rate of their susceptibility and also properties of the nano-silver solutions.

Full-Text [PDF 507 kb]   (1663 Downloads)    
Type of Study: Original | Subject: Antimicrobial Substances
Received: 2016/04/12 | Accepted: 2016/10/31

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