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:: year 11, Issue 3 (July - August 2017) ::
Iran J Med Microbiol 2017, 11(3): 19-26 Back to browse issues page
Molecular Detection of ISAba2 among Carbapenem Hydrolyzing Class D β-Lactamasese Acinetobacter baumannii Strains Isolated from Patients in Tehran Hospitals
Mina Owrang 1, Gita Eslami 2, Fatemeh Fallah 3, Shiva Irani 1, Mohammad Rahbar 4
1- Department of Biology, School of Basic Sciences, Science and Research branch, Islamic Azad University, Tehran, Iran
2- Department of Microbiology, Medical school, Shahid Beheshti University of Medical Science, Tehran, Iran , tahmasb@hotmail.com
3- Department of Microbiology, Medical school, Shahid Beheshti University of Medical Science, Tehran, Iran
4- Department of Microbiology, Reference Health Laboratories Research Centre, Ministry of Health and Medical Education, Tehran, Iran
Abstract:   (1771 Views)

Background and Aims: Acinetobacter baumannii is an opportunistic pathogen that has acquired a high rate of antibiotic resistance. Identification of the major elements increasing the expression of resistance genes while having a role in their transmission, can help us control the A. baumannii infections. This study aimed to determine the prevalence of ISAba2 in
A. baumannii strains which include group D beta-lactamase genes among hospitalized patients.
Materials and Methods: From August 2014 to April 2015, 105 A. baumannii strains were collected from different clinical samples of patients in 5 hospitals in Tehran. The confirmation of strains was done by phenotypical tests and existence of blaOXA-51-like gene. Antibiotic susceptibility pattern of the isolates were performed by Disc Diffusion Test (DDT) and Minimum Inhibitory Concentration (MIC) according to the CLSI. ESBL producing strains were recognized with Combined Disc Diffusion Test (CDDT) while the presence of OXA genes and ISAba1and ISAba2 were analyzed using PCR reactions.
Results: The result of this study showed that the highest and lowest rates of antibiotic resistance belonged to cefotaxim (100%) and colistin (99.05%), respectively. A total of 55 isolates (54.5%) were capable of producing ESBL. Unlike the blaOXA-58-like gene, which was not found in any of the isolates, blaOXA-51-like- was present among all the isolates.. Prevalence of blaOXA-23-like and blaOXA-24-like genes were 103 (98.09%) and 68 (64.76%), respectively and the frequency of ISAba1 and ISAba2 were 105 (100%) and 97 (92.36%), respectively.
Conclusions: The existence of additional elements as effective factors, can increase the expression of resistance genes and, therefore, help them to be mobile and transmitted between bacteria. Determination of these elements is, therefore, necessary for controlling infections.

Keywords: Acinetobacter baumannii, Antibiotic resistance, Carbapenems, CHDLs, ISAba2
Full-Text [PDF 588 kb]   (467 Downloads)    
Type of Study: Original | Subject: Medical Bacteriology
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Owrang M, Eslami G, Fallah F, Irani S, Rahbar M. Molecular Detection of ISAba2 among Carbapenem Hydrolyzing Class D β-Lactamasese Acinetobacter baumannii Strains Isolated from Patients in Tehran Hospitals . Iran J Med Microbiol. 2017; 11 (3) :19-26
URL: http://ijmm.ir/article-1-675-en.html


year 11, Issue 3 (July - August 2017) Back to browse issues page
مجله میکروب شناسی پزشکی ایران Iranian Journal of Medical Microbiology
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