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Iran J Med Microbiol 2017, 11(3): 27-36 Back to browse issues page
Adhesion Factors and Association with Antibiotic Resistance among Clinical Isolates of Staphylococcus aureus
Hamid Motamedi 1, Babak Asghari 1, Hamed Tahmasebi 2, Mohammad Reza Arabestani 3
1- Department of Microbiology, School of Medicine, University of Hamadan, Hamadan, Iran
2- Department of Microbiology, School of Medicine, University of Zahedan, Zahedan, Iran
3- Department of Microbiology, School of Medicine, University of Hamadan, Hamadan, Iran , mohammad.arabestani@gmail.com
Abstract:   (1544 Views)

Background and Aims: Staphylococcus aureus adhesion factors can reinforce the pathogenicity of the bacteria. The aim of this study was to identify adhesion factors among clinical isolates of methicillin-resistant S. aureus and determine the association between these factors and antibiotic resistance patterns.
Materials and Methods: In an analytical study from October 2016 to April 2017, 302 clinical isolates of S. aureus were confirmed by biochemical tests. Methicillin-resistant strains were determined by phenotypic methods. Multiplex PCR method was used to identify adhesion factors. In this way, bbp, cna, eno and ebpS genes were identified among different isolates.
Results: A total of 302 clinical isolates of S. aureus were isolated from different clinical samples including wound, blood, urine, trachea, catheter, swabs. Of 302 isolates, 123 were methicillin resistant and 73.53% and 75.7% of the isolates were resistant to erythromycin and penicillin, respectively. The incidence of resistance genes among among methicillin resistant S. aureus isolates were as follows: bbp (10 isolates: 6.89%), cna (6 isolates: 13.4%), eno (28 isolates: 19.31%) and ebpS (19 isolates: 13.1%),. There was a significant correlation between the antibiotic resistance patterns and the frequency of adhesion factors (P≤0.05).
Conclusions: According to the results, there was a significant correlation between adhesion factors and antibiotic resistance among methicillin-resistant isolates of S. aureus.
 

Keywords: Antibiotice Resistance, Methicilin Resistance of Staphylococcus aureus, Adhesion factors
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Type of Study: Original | Subject: Medical Bacteriology
References
1. Zarei Koosha R, Mahmoodzadeh Hosseini H, Mehdizadeh Aghdam E, Ghorbani Tajandareh S, Imani Fooladi AA. Distribution of tsst-1 and mecA Genes in Staphylococcus aureus Isolated From Clinical Specimens. Jundishapur J Microbiol. 2016; 9(3):1-8.
2. Agnoletti F, Mazzolini E, Bacchin C, Bano L, Berto G, Rigoli R, et al. First reporting of methicillin-resistant Staphylococcus aureus (MRSA) ST398 in an industrial rabbit holding and in farm-related people. Vet Microbiol 2014; 170(1–2):172-7.
3. Taylor AR. Methicillin-Resistant Staphylococcus aureus Infections. Primary Care: Clinics in Office Practice 2013; 40(3):637-54.
4. Khazaei S, Pourtahmaseby P, Kanani M, Madani SH, Malekianzadeh E. Staphylococcus aureus Resistance to Vancomycin: A Six Years Survey, (2006-2012). Med J Tabriz Univ Med Sci.2013; 5 )35(:40-45.
5. Sahebnasagh R, Saderi H, Owlia P. The Prevalence of Resistance to Methicillin in Staphylococcus aureus Strains Isolated from Patients by PCR Method for Detec-tion of mecA and nuc Genes. Iran J Public Health 2014; 43(1):84-92.
6. Loncaric I, Kubber-Heiss A, Posautz A, Stalder GL, Hoffmann D, Rosengarten R, et al. mecC- and mecA-positive meticillin-resistant Staphylococcus aureus (MRSA) isolated from livestock sharing habitat with wildlife previously tested positive for mecC-positive MRSA. Vet Dermatol 2014; 25(2):147-8.
7. Marshall H, Nissen M, Richmond P, Shakib S, Jiang Q, Cooper D, et al. Safety and immunogenicity of a booster dose of a 3-antigen Staphylococcus aureus vaccine (SA3Ag) in healthy adults: A randomized phase 1 study. J Infect 2016; 73(5):437-54.
8. Mackey-Lawrence NM, Jefferson KK. Regulation of Staphylococcus aureus immunodominant antigen B (IsaB). Microb Res 2013; 168(2):113-8.
9. Khorvash F, Abbasi S, Khomarbaghi N, Shokri D, Akhond MR, Rostami S. Investigating the Frequency of Staphylococcus aureus Colonization in Intensive Care Unit Patients in Admission and during Hospitalization, Alzahra Hospital, Isfahan, Iran. J Isfahan Med Sch 2013; 31(230): 364-71.
10. Khoramian B, Jabalameli F, Niasari-Naslaji A, Taherikalani M, Emaneini M. Comparison of virulence factors and biofilm formation among Staphylococcus aureus strains isolated from human and bovine infections. Microb Pathog 2015; 88(11):73-7.
11. Alli OA, Ogbolu DO, Shittu AO, Okorie AN, Akinola JO, Daniel JB. Association of virulence genes with mecA gene in Staphylococcus aureus isolates from Tertiary Hospitals in Nigeria. Indian J Pathol Microbiol 2015; 58(4):464-71.
12. Imani Fooladi AA, Ashrafi E, Tazandareh SG, Koosha RZ, Rad HS, Amin M, et al. The distribution of pathogenic and toxigenic genes among MRSA and MSSA clinical isolates. Microb pathog 2015; 81(4):60-6.
13. Srinivasan A, Bankowski MJ, Seifried SE, Jinno S, Perkins R, Singh S, et al. A probe-based method for confirmation of methicillin-resistant Staphylococcus aureus and detection of Panton–Valentine leukocidin and tst virulence genes. Diagn Microbiol Infect Dis 2011; 70(4):541-3.
14. Hu DL, Omoe K, Inoue F, Kasai T, Yasujima M, Shinagawa K, et al. Comparative prevalence of superantigenic toxin genes in meticillin-resistant and meticillin-susceptible Staphylococcus aureus isolates. JMed Microb 2008; 57(9):1106-12.
15. Tahmasebi h, Bokaeian m, Adabi j. Coagulase-Negative, Beta-lactam, antibiotic resistance, methicillin resistance. J Jahrom Univ Med Sci 2016; 14(1):55-63.
16. Bokaeian M, Adabi J, Zeyni B, Tahmasebi H. The Presence of aac (6 ') Ie / aph (2 "), aph (3') -IIIa1, ant (4 ')-Ia1 Genes and Determining Methicillin Resistance in Staphylococcus Epidermidis and Staphylococcus Saprophyticus Strains Isolated from Clinical Specimens. J Arak Univ Med Sci 2017; 19(11):11-25.
17. CLSI. M100-S25 performance standards for antimicrobial susceptibility testing; Twenty-fifth informational supplement; 2015.
18. Tristan A, Ying L, Bes M, Etienne J, Vandenesch F, Lina G. Use of Multiplex PCR To Identify Staphylococcus aureus Adhesins Involved in Human Hematogenous Infections. J CliniMicrobiol 2003; 41(9):4465-7.
19. Oryan G, Faghri J, Fazeli H, Hosseini NS, Sedighi M. Prevalence and Antibacterial Resistance of Coagulase Negative Staphylococci in Keratitis Infections Following the Use of Soft Contact Lenses. J Isfahan Med Sci 2014; 32(277): 273-81
20. Arabestani MR, Abdoli Kahrizi M. Determining the Agr Gene Variety (Accessory Gene Regulator) in Susceptible and Methicillin-Resistant Staphylococcus aureus Strains in Clinical Samples and Carriers Employed in Remedial Centers. J Arak Univ Med Sci 2016;18(11):44-53.
21. Havaei SA, Vidovic S, Tahmineh N, Mohammad K, Mohsen K, Starnino S, et al. Epidemic Methicillin-Susceptible Staphylococcus aureus Lineages Are the Main Cause of Infections at an Iranian University Hospital. J Clinl Microbiol 2011;49(11):3990-3.
22. Dibah S, Arzanlou M, Jannati E, Shapouri R. Prevalence and antimicrobial resistance pattern of methicillin resistant Staphylococcus aureus (MRSA) strains isolated from clinical specimens in Ardabil, Iran. Iran J Microbiol 2014;6(3):163-168.
23. Arabestani MR, Rastiany S, Mousavi SF, Ghafel S, Alikhani MY. Identification of toxic shock syndrom and exfoliative toxin genes of Staphylococcus aureus in carrier persons, resistant and susceptible methicillin. Tehran Univ Med J 2015;73(8):554-60.
24. Hajdu S, Holinka J, Reichmann S, Hirschl AM, Graninger W, Presterl E. Increased Temperature Enhances the Antimicrobial Effects of Daptomycin, Vancomycin, Tigecycline, Fosfomycin, and Cefamandole on Staphylococcal Biofilms. Antimicrob Agents Chemother 2010; 54(10):4078-84.
25. Goudarzi M, Seyedjavadi SS, Nasiri MJ, Goudarzi H, Sajadi Nia R, Dabiri H. Molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from patients with bacteremia based on MLST, SCCmec, spa, and agr locus types analysis. Microb Pathog 2017; 104(3):328-35.
26. Qi L, Li H, Zhang C, Liang B, Li J, Wang L, et al. Relationship between Antibiotic Resistance, Biofilm Formation, and Biofilm-Specific Resistance in Acinetobacter baumannii. Front Microbiol 2016;7 (4):483-493.
27. Agarwal A, Jain A. Association between drug resistance & production of biofilm in staphylococci. Indian J Med Res 2012; 135(4):562-564.
28. Schroeder M, Brooks BD, Brooks AE. The Complex Relationship between Virulence and Antibiotic Resistance. Genes 2017; 8(1):39.
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Motamedi H, Asghari B, Tahmasebi H, Arabestani M R. Adhesion Factors and Association with Antibiotic Resistance among Clinical Isolates of Staphylococcus aureus . Iran J Med Microbiol. 2017; 11 (3) :27-36
URL: http://ijmm.ir/article-1-707-en.html


year 11, Issue 3 (July - August 2017) Back to browse issues page
مجله میکروب شناسی پزشکی ایران Iranian Journal of Medical Microbiology
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