Molecular Diagnosis of Mycoplasma pneumoniae in Synovial Fluid of Rheumatoid Arthritis Patients

Golmohammadi, Reza; Ataee, Ramezan Ali; Alishiri, Gholam Hossein; Mirnejad, Reza; Najafi, Ali; Tate, Mehdi; Esmaieli, Davoude; Jonaidi Jafari, Nematollah

year 8, Issue 1 (Spring 2014) Iran J Med Microbiol 2014, 8(1): 1-8 | Back to browse issues page

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Golmohammadi R, Ataee R A, Alishiri G H, Mirnejad R, Najafi A, Tate M, et al . Molecular Diagnosis of Mycoplasma pneumoniae in Synovial Fluid of Rheumatoid Arthritis Patients. Iran J Med Microbiol 2014; 8 (1) :1-8
URL: http://ijmm.ir/article-1-276-en.html

Molecular Diagnosis of Mycoplasma pneumoniae in Synovial Fluid of Rheumatoid Arthritis Patients

Reza Golmohammadi¹

, Ramezan Ali Ataee

², Gholam Hossein Alishiri³

, Reza Mirnejad⁴

, Ali Najafi⁵

, Mehdi Tate⁶

, Davoude Esmaieli⁷

, Nematollah Jonaidi Jafari⁸

1- Department of Medical Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
2- Department of Medical Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran , ataee@bmsu.ac.ir
3- Department of Rheumatology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
4- Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
5- Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
6- Applied Virological Research, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
7- Department of Medical Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
8- Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

Abstract: (29327 Views)

Background and Aim: The role of Mycoplasma super antigens in inflammatory diseases such as rheumatoid arthritis (RA) is taken into consideration. Therefore, the aim of this study was to diagnosis of Mycoplasma pneumoniae in synovial fluid (SF) of patients with RA.

Materials and Methods: In this study, 131 synovial fluid samples of patients with RA were collected during 3 years (2011- 2013). Mycoplasma pneumoniae ATCC: 29342 strain was used as control. The genome of samples is extracted separately and PCR protocol by M. pneumoniae species and mycoplasma genus identifier primers was carried out. The PCR product was confirmed by sequencing.

Results: The results of this study showed that this molecular diagnosis method is able to detect M. pneumoniae in the synovial fluid of patients with RA. So that M. pneumoniae species was confirmed in 30 samples (22.9%) and Mycoplasma genus detected in the 70 sample (53.4%).

Conclusions: based on the results of this study, the PCR molecular method was able to detect of the Mycoplasma genus and also M. pneumoniae species in the synovial fluid of patients with RA. The results indicated that M. pneumoniae can be a probable cause of rheumatoid arthritis disease. This finding may help the specific treatment of RA.

Keywords: Mycoplasma pneumoniae, PCR, Rheumatoid arthritis, Synovial fluid

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Type of Study: Original Research Article | Subject: Molecular Microbiology
Received: 2014/05/12 | Accepted: 2014/06/11 | ePublished: 2014/06/11