Cytotoxic effects of Ecovirus-6, Lactobacillus casei and Bacillus coagulans concurrent treatment with hyperthermia on human lung cancer

Esmaeili Gouvarchin Ghaleh, Hadi; Eslamimahmoudabadi, Mostafa; Bolorian, Alireza; Zahiri, Ali; Asghardost Rezaee, Milad; Dorostkar, Ruhollah; Golmohammadi, Reza

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Cytotoxic effects of Ecovirus-6, Lactobacillus casei and Bacillus coagulans concurrent treatment with hyperthermia on human lung cancer

Hadi Esmaeili Gouvarchin Ghaleh¹

, Mostafa Eslamimahmoudabadi²

, Alireza Bolorian²

, Ali Zahiri³

, Milad Asghardost Rezaee²

, Ruhollah Dorostkar¹

, Reza Golmohammadi⁴

1- Applied Virology Research Center, Biomedicine Technologies Institute, Baqiyatallah University of Medical sciences, Tehran, Iran
2- Student Research Committee, Baqiyatallah University of Medical Sciences, Tehran, Iran
3- Baqiyatallah Research Center for Gastroenterology and Liver Diseases (BRCGL), Clinical Sciences Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
4- Baqiyatallah Research Center for Gastroenterology and Liver Diseases (BRCGL), Clinical Sciences Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran , rsr.golmohammadi@bmsu.ac.ir

Abstract: (127 Views)

Background & Objective: Although there have been significant improvements in the treatment of lung cancer, surgery continues to be the most effective approach, frequently used alongside radiotherapy and chemotherapy. The presence of challenges such as drug resistance, adverse effects, and insufficient selectivity has led researchers to explore new targeted therapies. A particularly promising strategy involves the application of oncolytic viruses (OVs) and bacteria. This research intends to assess the anti-proliferative effects of Ecovirus-6, Lactobacillus casei (L. casei), and Bacillus coagulans (B. coagulans) when administered together with hyperthermia on human lung cancer under cell culture conditions.
Methods: In this research, following the cultivation of A549 cells, the IC₅₀ concentration of Ecovirus-6, L. casei, and B. coagulans was established. Subsequently, the viability of the cells, the percentage of apoptosis, the production of reactive oxygen species (ROS), and the release of lactate dehydrogenase (LDH) were assessed for both single-agent and concurrent treatments.
Results: The findings of the research indicated that Ecovirus-6, L. casei, B. coagulans, and doxorubicin significantly reduce cell viability (P<0.001) while enhancing the percentage of apoptosis, as well as the production of ROS and LDH in lung cancer cells when compared to the control group (P<0.05). Furthermore, the results demonstrated that the concurrent treatment group exhibited greater effects than the groups treated with single agents and doxorubicin (P<0.05).
Conclusion: The current research demonstrated that the combination of Echovirus-6, L. casei, and B. coagulans with hyperthermia can effectively hinder the proliferation of lung cancer cells.