Evaluation of The Effects of Allium Jesdianum Bacterial Endophytes on Some Bacterial and Fungal Pathogens

Esmaeili, Maryam; Ebrahimi Kahrizsangi, Azizollah; Shahrokh Shahraki, Somayeh; Mokhtari, Azam

doi:10.30699/ijmm.15.3.281

year 15, Issue 3 (May - Jun 2021) Iran J Med Microbiol 2021, 15(3): 281-301 | Back to browse issues page

‎ 10.30699/ijmm.15.3.281

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Esmaeili M, Ebrahimi Kahrizsangi A, Shahrokh Shahraki S, Mokhtari A. Evaluation of The Effects of Allium Jesdianum Bacterial Endophytes on Some Bacterial and Fungal Pathogens. Iran J Med Microbiol 2021; 15 (3) :281-301
URL: http://ijmm.ir/article-1-1222-en.html

Evaluation of The Effects of Allium Jesdianum Bacterial Endophytes on Some Bacterial and Fungal Pathogens

Maryam Esmaeili¹

, Azizollah Ebrahimi Kahrizsangi¹

, Somayeh Shahrokh Shahraki

², Azam Mokhtari¹

1- Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
2- Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran , Shahrokh@sku.ac.ir

Abstract: (3872 Views)

Background and Objective: The endophytes are the fungi or bacteria that live within a plant in a symbiotic relationship. This study aimed to investigate the effect of isolated bacterial endophytes from Allium jesdianum on some bacterial pathogens such as Staphylococcus aureus, Escherichia coli as well as pathogenic fungi, including Trichophyton mentagrophytes and Candida albicans.
Materials and Methods: Various parts of A. jesdianum including leaves, stems, onions and flowers were randomly collected, cleaned from any contamination and then cultured in a suitable culture medium. The endophyte colonies were purified using chloroform to analyze the anti-pathogenic properties of the structural agents of the bacterial endophyte. To evaluate the anti-pathogenicity of endophyte metabolites, the broth media of the endophyte bacteria was centrifuged and the supernatants were filtered through sterilization. Antibiogram test was performed by disk diffusion method (Kirby Bauer) and the sensitivity was compared. Data were statistically analyzed by t-test and ANOVA.
Results: Eleven bacterial endophytes were obtained from various sections of A. jesdianum. The endophyte bacterium has an improved antimicrobial effect on T. mentagrophytes. Unlike E. coli, secretory metabolites of endophytic bacteria had an antimicrobial effect on S .aureus and C. albicans.
Conclusion: It can be concluded that the bacterial endophytes of A. jesdianum can be considered to be potential and beneficial agents against human pathogens. A. jesdianum with anti-pathogenic activity could be a source to produce important anti-pathogenic compounds from an agricultural and pharmaceutical point of view.

Keywords: Allium jesdianum, Anti-pathogenic activity, Endophyte, Medicinal plants, Microbial Sensitivity Tests

Full-Text [PDF 1229 kb] (1447 Downloads) | | Full-Text (HTML) (1297 Views)

Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2020/09/18 | Accepted: 2020/12/21 | ePublished: 2021/06/28

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