fa گزارشی از انتشار کلونال سویه جدیدی از ویبریو کلرا سروتیپ O1 بیوتیپ التور در شمال غرب کشور در تابستان 1386 باکتری شناسی پزشکی Medical Bacteriology مقاله پژوهشی Original Research Article <b>Background and Objectives: </b>Cholerae disease caused by toxigenic<i> V. cholerae</i>, is a major public health problem in developing countries including Iran. Epidemiological surveillance and comparative molecular analysis of isolates have demonstrated clonal diversity among epidemic strains and a continual emergence of new clones of toxigenic<i> V.cholerae. </i><br><b>Material and Methods:</b> A total of 20 <i>V.cholerae</i> strains were sent to Pasteur Institute of Iran in September of 2007 from Kordestan province which includes strains of different sub-serotypes. After biochemical identification, isolates subjected to molecular analysis including Pulsed-Field Gel Electrophoresis (PFGE) of NotI digested genomic DNA according to the standardized protocol by Centre of Disease Control (CDC). <br><b>Results: </b>PFGE results showed a single pattern for all isolates. <br> <b>Conclusion: </b>The results were interpreted in comparison with patterns obtained by isolates of previous years and showed clonal dissemination of a new clone in Kordestan province in this year. <b>Background and Objectives: </b>Cholerae disease caused by toxigenic V. cholerae, is a major public health problem in developing countries including Iran. Epidemiological surveillance and comparative molecular analysis of isolates have demonstrated clonal diversity among epidemic strains and a continual emergence of new clones of toxigenic <i>V.cholerae</i>. <br><b>Material and Methods:</b> A total of 20 <i>V.cholera</i>e strains were sent to Pasteur Institute of Iran in September of 2007 from Kordestan province which includes strains of different sub-serotypes. After biochemical identification, isolates subjected to molecular analysis including Pulsed-Field Gel Electrophoresis (PFGE) of NotI digested genomic DNA according to the standardized protocol by Centre of Disease Control (CDC). <br><b>Results: </b>PFGE results showed a single pattern for all isolates. <br> <b>Conclusion: </b>The results were interpreted in comparison with patterns obtained by isolates of previous years and showed clonal dissemination of a new clone in Kordestan province in this year. ویبریو کلرا، کردستان، PFGE، اینابا Vibrio cholerae, Kurdastan, PFGE, Inaba, 2007 67 69 http://ijmm.ir/browse.php?a_code=A-10-1-63&slc_lang=fa&sid=1 Bita Bakhshi بیتا بخشی 1003194753284600386 1003194753284600386 No Department of Bacteriology, Pasteur Institute ofIran بخش باکتری شناسی، انستیتو پاستور ایران Habibollah Naseri حبیب اله ناصری 1003194753284600387 1003194753284600387 No Health Centre of Kurdestan province, Kordestan University of Medical sciences seyed mohammad Zahraei سیدمحسن زهرایی 1003194753284600388 1003194753284600388 No Center for Disease Control, Department of Food borne and Water borne disease, Tehran Mohhamad Afshani محمدتقی افشانی نقده 1003194753284600389 1003194753284600389 No Center for Disease Control, Department of Food borne and Water borne disease, Tehran Mohammadreza Pourshafie محمدرضا پورشفیع pour@pasteur.ac.ir 1003194753284600390 1003194753284600390 Yes Department of Bacteriology, Pasteur Institute ofIran بخش باکتری شناسی، انستیتو پاستور ایران