en باکتری شناسی پزشکی Medical Bacteriology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Background and Aim:</span></span></span></b><span lang="EN-ID">&nbsp;The microbial flora colonizing the pilosebaceous unit plays a crucial role in maintaining skin health and homeostasis. This study aimed to isolate, identify, and evaluate the biofilm-forming capacity of bacterial strains derived from the pilosebaceous follicles of healthy human facial skin.</span><br> <b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Materials and Methods:</span></span></span></b> <span lang="EN-ID">Facial sebum samples were collected from 15 healthy volunteers. Bacterial strains were isolated and cultured under anaerobic conditions. Species identification was performed using 16S rRNA gene sequencing. The biofilm-forming ability of the isolates was quantified using the crystal violet staining assay.</span><span style="color:black"> </span><br> <b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Results:</span></span></span></b> <span lang="EN-ID">A total of 22 bacterial strains were isolated, predominantly Gram-positive, nonmotile, and morphologically characterized as rod-shaped (20/22) or coccoid (2/22). Colonies appeared opaque and white, with diameters ranging from 0.5 to 1.5 mm. Biochemical profiling showed that most isolates were catalase positive (18/22) and gelatinase positive (20/22), while the majority were oxidase negative (18/22). Three isolates exhibited lipase activity, and eight demonstrated hemolysin production. Based on 16S rRNA gene sequencing, <em>Cutibacterium</em> was identified as the dominant genus, with <em>C. acnes</em> accounting for 68% of the isolates. Biofilm assays revealed variability among <em>C. acnes </em>strains, with isolates Hn15-2, Hn4, and Hn13 displaying strong biofilm-forming capacity.</span><br> <b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Conclusion:</span></span></span></b> <span lang="EN-ID">Healthy human facial skin harbors a diverse bacterial community, predominantly composed of <em>Cutibacterium acnes</em>. Notably, several <em>C. acnes </em>isolates demonstrated substantial biofilm-forming ability, suggesting potential implications for skin microbiome stability and pathogenicity.</span></span></span></p> Acne vulgaris, Biofilm, Cutibacterium acnes, Facial Skin Microbiota 244 253 http://ijmm.ir/browse.php?a_code=A-10-2884-3&slc_lang=en&sid=1 Xuong Mau Tu tumauxuong921@gmail.com 100319475328460033972 0009-0008-4481-5128 No Institute of Food and Biotechnology, Can Tho University, Can Tho City, Vietnam Minh Binh Nguyen Le lenguyenbminh@gmail.com 100319475328460033973 100319475328460033973 No Institute of Food and Biotechnology, Can Tho University, Can Tho City, Vietnam Hau Huu Tran thhau@ctu.edu.vn 100319475328460033974 0009-0001-9234-2694 No Institute of Food and Biotechnology, Can Tho University, Can Tho City, Vietnam Thanh Ngoc Nguyen nnthanh@ctu.edu.vn 100319475328460033975 100319475328460033975 No Institute of Food and Biotechnology, Can Tho University, Can Tho City, Vietnam Long Dang Hoang Bui bhdlong@ctu.edu.vn 100319475328460033976 100319475328460033976 No Institute of Food and Biotechnology, Can Tho University, Can Tho City, Vietnam Dung Ngoc Tran dungctump@gmail.com 100319475328460033977 100319475328460033977 No Can Tho University of Medicine and Pharmacy, Can Tho City, Vietnam Sinh Nhan Ho Hosinhnhan235@gmail.com 100319475328460033978 100319475328460033978 No Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Vietnam Phong Xuan Huynh hxphong@ctu.edu.vn 100319475328460033979 100319475328460033979 Yes Institute of Food and Biotechnology, Can Tho University, Can Tho City, Vietnam