en باکتری شناسی پزشکی Medical Bacteriology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:16px;"><b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Background and Aim:</span></span></span></b> <span lang="EN-IN"><span style="color:black">The most effective antibiotic for treating methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) infections is vancomycin. However, vancomycin-intermediate <i>Staphylococcus (S.) aureus</i> (VISA) and vancomycin-resistant <i>S. aureus</i> (VRSA) have been found to be more common in various parts of the world. This study aimed to determine the prevalence of <i>mecA</i>, <i>vanA</i> and <i>vanB</i> genes among MRSA isolates and to categorize vancomycin susceptibility profiles in clinical specimens from TMMC & RC, Moradabad.</span></span><br> <b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Materials and Methods:</span></span></span></b> <span lang="EN-IN"><span style="color:black">In the central laboratory of TMMC and RC Moradabad, <i>S. aureus</i> was isolated from various clinical samples and MRSA was detected by Kirby-Bauer disk diffusion method. Minimum inhibitory concentration (MIC) of vancomycin was determined by broth microdilution (BMD) method and categorized it into VSSA, VISA, and VRSA isolates. The <i>mecA</i>, <i>vanA</i> and <i>vanB</i> genes were detected on extracted genomic DNA using polymerase chain reaction.</span></span> <span style="color:black"></span><br> <b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Results:</span></span></span></b> <span lang="EN-IN"><span style="color:black">Out of 314 MRSA strains, 296 (94.27%) were VSSA, 14 (4.46%) were VISA and 4 (1.27%) were VRSA, as found on the basis of MIC determination through BMD method. All MRSA strains possessed the <i>mecA</i> gene, 1 VRSA had <i>vanA</i> and 3 VRSA had <i>vanB</i>. Among VISA isolates no <i>vanA</i> and <i>vanB</i> genes were detected.</span></span><span style="color:black"></span><br> <b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Conclusion:</span></span></span></b> <span lang="EN-IN"><span style="color:black">The high prevalence of MRSA (52.5%) and the emergence of VRSA (1.27%) carrying <i>vanA</i> and <i>vanB</i> genes in our setting highlight a serious public health threat and underscore the need for continuous surveillance and stringent infection control measures.</span></span></span></span></p> Cefoxitin, Methicillin-resistant Staphylococcus aureus, Staphylococcus aureus, Vancomycin, Vancomycin-intermediate Staphylococcus aureus, Vancomycin-resistant Staphylococcus aureus 354 368 http://ijmm.ir/browse.php?a_code=A-10-2865-1&slc_lang=en&sid=1 Shane Alam shanealam5361@gmail.com 100319475328460033732 0009-0006-0485-1832 Yes Department of Microbiology, Teerthanker Mahaveer University, Moradabad (U.P.), India Umar Farooq farooqzf@yahoo.com 100319475328460033733 0009-0003-0697-0681 No Department of Microbiology, Teerthanker Mahaveer University, Moradabad (U.P.), India