en ژنتیک میکروبی Microbial Genetics مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Background and Aim:</span></span></span></b><span lang="EN-ID">&nbsp;Biofilm formation is a key factor in persistence and antibiotic resistance of <em>Pseudomonas (P.) aeruginosa</em>, especially in intensive care units (ICU). This study aimed to explore how common the <em>pslA</em> and <em>pslB</em> genes are in multidrug-resistant (MDR) strains of <em>P. aeruginosa</em> isolated from ICU patients in Tehran and how these genes relate to biofilm development.</span><br> <b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Materials and Methods:</span></span></span></b> <span lang="EN-ID">For this cross-sectional study, 112 <em>P. aeruginosa</em> isolates were collected from patients admitted to the ICUs of 1000-bed tertiary care of Milad Hospital, Tehran, Iran. The isolates were identified using standard microbiological techniques. To assess biofilm formation, crystal violet staining was conducted using 96-well microtiter plate. DNA was extracted using a commercial kit, and polymerase chain reaction (PCR) was carried out to detect the <em>pslA/B</em> genes.</span><span style="color:black"> </span><br> <b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Results:</span></span></span></b> <span lang="EN-ID">Of 112 samples, 92 (82.1%) isolates were biofilm producers, among them the vast majority of biofilm-producing strains were tested positive for both <em>pslA</em> (91, 98.9%) and <em>pslB</em> (92, 100%) genes. One <em>pslA</em>-negative isolate from a wound specimen retained weak biofilm-forming capacity. These genes showed a strong association with biofilm development (<em>P</em><0.05).</span><br> <b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Conclusion:</span></span></span></b> <span lang="EN-ID">The high prevalence of <em>pslA/B</em> genes in biofilm-forming MDR isolates suggests their significant role in enhancing biofilm formation and antibiotic resistance. This highlights the need to understand biofilm-related genes in managing ICU infections, though methodological limitations warrant further validation.</span></span></span></p> Biofilm, ICU, Multidrug Resistance, Pseudomonas aeruginosa, pslA, pslB 150 158 http://ijmm.ir/browse.php?a_code=A-10-1602-7&slc_lang=en&sid=1 Mohammad Esmaeil Amini moh.es.amini@gmail.com 100319475328460032321 100319475328460032321 No Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Shabnam Razavi shabnam_razavi9@yahoo.com 100319475328460032322 100319475328460032322 No Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Mohammad Rahbar rahbar.reflab@gmail.com 100319475328460032323 100319475328460032323 No Department of Microbiology, Ministry of Health & Medical Education, Reference Health Laboratory Research Center, Tehran, Iran Parisa Eslami Pm_eslami@yahoo.com 100319475328460032324 0009-0002-7028-3758 No Department of Microbiology, Milad Hospital, Tehran, Iran Abed Zahedi bialvaei abedzahedi@gmail.com 100319475328460032325 100319475328460032325 Yes Microbial Biotechnology Research Center, Iran University of Medical Sciences, Tehran, Iran