en باکتری شناسی پزشکی Medical Bacteriology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Background and Aim:</span></span></span></b> <span lang="EN-ID">One of the animal models for bacterial virulence testing is<em> Omphisa (O.) fuscidentalis</em>. The purpose of this study was to evaluate<em> O. fuscidentalis</em> larvae as an animal model for bacterial virulence testing in healthy individuals and hospitalized patients.</span><br> <b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Materials and Methods:</span></span></span></b> <span lang="EN-ID">The design of this study was experimental. Bacterial samples were obtained from Mitra Keluarga Hospital, Bekasi, Indonesia. Molecular identification of bacteria was confirmed using partial <em>16S rRNA</em> gene sequencing. Identification and susceptibility testing of bacteria was performed using Vitek 2 compact. The dose treatment of bacterial isolates <em>Staphylococcus (S.) haemolyticus, Enterococcus (E.) faecalis</em>, and <em>Klebsiella (K.) pneumoniae</em> (103, 104, 105) was carried out on survivability of <em>O. fuscidentalis</em> larvae over 24 hr post-injection.</span><span style="color:black"> </span><br> <b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Results:</span></span></span></b> <span lang="EN-ID"><em>Klebsiella pneumoniae</em> strains were resistant to more than five classes of antibiotics. The lowest survival percentage was obtained in <em>O. fuscidentalis</em> larvae injected with <em>K. pneumoniae</em>. Microscopic observation of dead <em>O. fuscidentalis</em> larvae showed hemocytes from melanized larvae exhibited swelling.</span><br> <b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Conclusion:</span></span></span></b> <span lang="EN-ID">Although this research is still basic, <em>O. fuscidentalis</em> larvae showed to be a probable promising alternative model to assess bacterial virulence. However, comparative studies with established test animals are needed.</span></span></span></p> Virulence, Omphisa fuscidentalis, Animal Model, Multi-drug Resistant, Hemocytes, Galleria mellonella 159 170 http://ijmm.ir/browse.php?a_code=A-10-2763-1&slc_lang=en&sid=1 Maulin Inggraini maulin.inggraini@stikesmitrakeluarga.ac.id 100319475328460032375 0009-0009-1660-8823 Yes Department of Medical Laboratory Technology, STIKes Mitra Keluarga, Bekasi City, Indonesia Noor Andryan Ilsan noor.andryan@stikesmitrakeluarga.ac.id 100319475328460032376 100319475328460032376 No Department of Medical Laboratory Technology, STIKes Mitra Keluarga, Bekasi City, Indonesia Ni Putu Ratna Ayu Krishanti nipu003@brin.go.id 100319475328460032377 0009-0008-0000-2619 No Research Center for Applied Zoology, National Research and Innovation Agency, Soekarno Science and Techno Park, Bogor, Indonesia Jepri Agung Priyanto jepriyanto@apps.ipb.ac.id 100319475328460032378 100319475328460032378 No Division of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University (IPB University), Bogor, Indonesia Reza Anindita rezaanindita@stikesmitrakeluarga.ac.id 100319475328460032379 0009-0000-7037-9317 No Department of Pharmacy, STIKes Mitra Keluarga, Bekasi City, Indonesia Fhasya Algina Hawatul Haq fhasyaalgina01@gmail.com 100319475328460032380 100319475328460032380 No Department of Medical Laboratory Technology, STIKes Mitra Keluarga, Bekasi City, Indonesia Oktavia Dwi Mauria oktaviadm26@gmail.com 100319475328460032381 100319475328460032381 No Department of Medical Laboratory Technology, STIKes Mitra Keluarga, Bekasi City, Indonesia Salsabila Ranmika Sari ranmikasalsabila@gmail.com 100319475328460032382 100319475328460032382 No Department of Nursing, STIKes Mitra Keluarga, Bekasi City, Indonesia Najla Salsabila Rahmadina najlarahmadina997@gmail.com 100319475328460032383 100319475328460032383 No Department of Nutrition, STIKes Mitra Keluarga, Bekasi City, Indonesia