en مواد ضد میکروبی Antimicrobial Substances مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Background and Aim:</span></span></span></b><span lang="EN-ID">&nbsp;Quercetin is a plant-derived flavonoid with diverse biological and pharmacological properties, including antioxidant, anti-inflammatory, anticancer, antibacterial, antifungal, and antiviral activities. Flavonoid compounds such as quercetin have demonstrated therapeutic potential against several cancer types, including liver, colorectal, and breast cancers. This study aimed to evaluate the anticancer, antibacterial, and antifungal activities of thiourea derivative of quercetin.</span><br> <b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Materials and Methods:</span></span></span></b> <span lang="EN-ID">The biological activities of the quercetin thiourea derivative were assessed against selected bacterial and fungal strains, as well as liver cancer cells. The minimum inhibitory concentration (MIC) was determined using the agar well diffusion method. Anticancer activity against liver cancer cells was evaluated using the MTT assay.</span><span style="color:black"> </span><br> <b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Results:</span></span></span></b> <span lang="EN-ID">The quercetin thiourea derivative exhibited antibacterial activity against<em> Bacillus cereus</em> (MW972221.1), <em>Burkholderia</em> spp., <em>Staphylococcus aureus</em>, and <em>Enterococcus faecalis</em>, with MIC values of 1, 15, 10, and 0.8 mg/mL, respectively. It also demonstrated antifungal activity against<em> Candida</em> species, with MICs of 5 mg/mL for <em>C. krusei </em>and <em>C. tropicalis</em>, 1 mg/mL for <em>C. albicans</em>, and 30 mg/mL for<em> C. glabrata</em>. The compound showed cytotoxic effects on liver cancer cells, with IC₅₀ values of 300, 224, and 31.5 &mu;g/mL at 24, 48, and 72 hours, respectively.</span><br> <b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Conclusion:</span></span></span></b> <span lang="EN-ID">The quercetin thiourea derivative represents a biologically active compound with promising antibacterial, antifungal, and anticancer properties. Its inhibitory effect on liver carcinoma cell growth is likely mediated through apoptosis induction. These findings suggest that the quercetin thiourea derivative could serve as a potential lead compound for antimicrobial and anticancer drug development, warranting further investigation.</span></span></span></p> Antibacterial, Anticancer, Antifungal, Liver Cancer, MIC, Quercetin, Quercetin Derivatives 230 243 http://ijmm.ir/browse.php?a_code=A-10-2880-1&slc_lang=en&sid=1 Ban M. Saeed ban.saeed@uobasrah.edu.iq 100319475328460033292 100319475328460033292 Yes Department of Microbiology, Al-Zahraa College of Medicine, University of Basrah, Basrah, Iraq Farqad M. Alhamdani farqad.mohsson@uobasrah.edu.iq 100319475328460033293 100319475328460033293 No Department of Microbiology, Al-Zahraa College of Medicine, University of Basrah, Basrah, Iraq Shaker A.N. Al-Jadaan sh.aljadaan@uobasrah.edu.iq 100319475328460033294 100319475328460033294 No Department of Pharmaceutical Chemistry, College of Pharmacy, University of Basrah, Basrah, Iraq Basil A. Abbas basil.abbas@uobasrah.edu.iq 100319475328460033295 100319475328460033295 No Department of Microbiology, College of Veterinary Medicine, University of Basrah, Basrah, Iraq