en میکروب شناسی دندان پزشکی Oral Microbiology مقاله کوتاه پژوهشی Brief Original Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Background and Aim:</span></span></span></b><span style="color:black"> Prolonged use of chemical agents to control cariogenic bacteria is frequently associated with adverse side effects. Consequently, increasing attention has been directed toward replacing conventional chemical antimicrobials with herbal alternatives. This study aimed to evaluate the <i>in vitro</i> antibacterial and anti-biofilm activities of curcumin nanoparticles against standard strains of <i>Lacticaseibacillus (L.) casei</i> and <i>Lactobacillus (L.) acidophilus</i>.</span><span lang="EN-ID"><span style="color:black"></span></span><br> <b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Materials and Methods:</span></span></span></b><span style="color:black"> In this descriptive cross-sectional study, the minimum inhibitory concentration of nano-curcumin was determined and compared with that of chlorhexidine using broth microdilution method. Chlorhexidine mouthwash served as the positive control, whereas physiological saline was used as the negative control. MICs were determined in 96-well microplates according to the Clinical and Laboratory Standards Institute guidelines. Anti-biofilm activity was assessed by a microtiter plate assay employing crystal violet staining. Data were analyzed using SPSS version 22, with statistical significance defined as <i>P</i><0.05.</span><br> <b><span style="background:#1f497d"><span style="color:white"><span style="letter-spacing:-.1pt">Results & Conclusion:</span></span></span></b><span style="color:black"> Chlorhexidine inhibited biofilm formation at concentrations of 6.25 &micro;g/mL for <i>L. acidophilus</i> and 12.5 &micro;g/mL for <i>L. casei</i>. Notably, nan-ocurcumin reduced biofilm formation significantly in both species (<i>P</i><0.05) at a sub-MIC concentration of 3.125 &micro;g/mL. Overall, curcumin nanoparticles exhibited distinct antibacterial and anti-biofilm effects against <i>L. casei</i> and <i>L. acidophilus</i>. Given their natural origin, favorable safety profile, and potential to overcome the limitations of synthetic antimicrobials, nan-ocurcumin formulations may represent a promising and biocompatible alternative for the prevention of dental caries.</span></span></span></p> Antimicrobial, Biofilm, Curcumin Nanoparticle, Lactobacillus acidophilus, Lacticaseibacillus casei 369 376 http://ijmm.ir/browse.php?a_code=A-10-2713-1&slc_lang=en&sid=1 Amirhossein Rajabnia ramazan69@yahoo.com 100319475328460033734 100319475328460033734 No Dental Research Center, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran Ezzat Allah Ghaemi eghaemi@yahoo.com 100319475328460033735 100319475328460033735 No Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran Ezatolah Kazeminejad Ezztk.edu@gmail.com 100319475328460033736 100319475328460033736 Yes Dental Research Center, Golestan University of Medical Sciences, Gorgan, Iran