en میکروب شناسی مولکولی Molecular Microbiology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><span style="color:#ffffff;"><b><span style="background:#1f497d"><span style="letter-spacing:-.1pt">Background and Aim:</span></span></b></span> Faba bean (<i>Vicia faba</i> L.) is an inseparable part of the Iraqi people&#39;s food basket. Root rot disease is one of the pests causing great losses to crops. The study aimed to determine the molecular characterization of the root rot fungi isolated from the roots of broad beans in Maysan Governorate, southern Iraq.<br> <span style="color:#ffffff;"><b><span style="background:#1f497d"><span style="letter-spacing:-.1pt">Materials and Methods:</span></span></b></span> Eight species of root rot fungi were isolated and subjected to DNA extraction and PCR assays. The sequences were submitted to GenBank and deposited using the assigned accession numbers.<br> <span style="color:#ffffff;"><b><span style="background:#1f497d"><span style="letter-spacing:-.1pt">Results:</span></span></b> </span>The study showed a match between phenotypic and molecular identification. All tested<i> </i>isolates produced fragments of 500&ndash;700 bp. The isolates&#39; sequences showed 98.40 to 100% matching to the reference strain sequences in GenBank. <i>Myrothecium (M.) inundatum</i> and <i>Mucor (M.) fragilis</i> showed 100% identity and <i>Corynascus (C.) sepedonium</i>, <i>Fusarium (F.) oxysporum </i>and <i>Fusarium (F.) solani</i> presented more than 99% identity. The strain <i>C. sepedonium </i>showed 100% identity with MZ203485.1 reference strain. The <i>F. oxysporum</i> indicated 100% similarity with several strains such as OR879163.1 and OR879157.1. The <i>F. solani</i> showed 100% similarity with MN857747.1 and MT509567.1 strains. <i>Macrophomina (M.) phaseolina</i> was the closest to MT127390.1 strain. The <i>M. fragilis</i> showed 100% identity with many reference strains. <i>M. inundatum</i><b> </b>revealed 100% similarity with both OP761606.1 and MK024176.1 strains, and <i>Ovatospora (O.) pseudomollicella</i> indicated 100% similarity with MW251835.1 and MW242833.1 strains.<i> Rhizoctonia (R.) solani</i> showed 100% similarity with many strains such as AF153786 and MH014991.1.<br> <span style="color:#ffffff;"><b><span style="background:#1f497d"><span style="letter-spacing:-.1pt">Conclusion:</span></span></b> </span>The PCR-RFLP assay is a favorable method for identification of the fungi causative of the root rot disease in accompany with phenotypic identification.</span></span></p> Broad Bean, Molecular Identification, Phylogenetic Tree, Rhizoctonia solani, Root Rot Fungi 62 73 http://ijmm.ir/browse.php?a_code=A-10-2709-1&slc_lang=en&sid=1 Asia N Kadim scipg2206@uomisan.edu.iq 100319475328460031170 100319475328460031170 No Department of Biology, College of Science, University of Misan, Maysan, Iraq Ali A Kasim alimycol@uomisan.edu.iq 100319475328460031171 100319475328460031171 Yes Department of Biology, College of Science, University of Misan, Maysan, Iraq Ghassan Mahdi Daghir aldaghir63@gmail.com 100319475328460031172 100319475328460031172 No Department of Plant Protection, College of Agriculture, University of Misan, Maysan, Iraq