en مواد ضد میکروبی Antimicrobial Substances مقاله کوتاه پژوهشی Brief Original Article <p>&nbsp;</p> <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><span style="color:#ffffff;"><b><span style="background:#1f497d"><span style="letter-spacing:-.1pt">Background and Aim:</span></span></b></span> Honey is a natural substance with potential applications as an alternative to preservatives and antibiotics due to its diverse biological activities. This study aimed to investigate the antioxidant, antimicrobial, and anti-biofilm activities of some Iranian honey samples.<br> <span style="color:#ffffff;"><b><span style="background:#1f497d"><span style="letter-spacing:-.1pt">Materials and Methods:</span></span></b> </span>Eight Iranian honey samples with different floral origins were selected and their physicochemical characteristics were determined. Antioxidant activity was assessed using DPPH assay. Antimicrobial activity was evaluated against <i>Staphylococcus (S.) aureus</i>, <i>Bacillus (B.) cereus</i>, <i>Escherichia (E.) coli</i>, and <i>Pseudomonas (P.) aeruginosa</i> using agar well diffusion and microdilution methods. Anti-biofilm activity was determined by the crystal violet staining method.<br> <span style="color:#ffffff;"><b><span style="background:#1f497d"><span style="letter-spacing:-.1pt">Results & Conclusion:</span></span></b> </span>The honey samples showed different physicochemical characteristics, most of which complied with international standards. The antioxidant activity of samples at 500 mg/ml concentration was in the range of 48-64%. All honey samples showed antibacterial activity. The minimum inhibitory concentration (MIC) values of honeys ranged from &le;3.125 to >50% (w/v). The highest antibacterial activity was observed for the <i>Fraxinus excelsior</i> and <i>Astragalus-Euphorbia</i> honeys, and <i>Glycyrrhiza glabra</i> honey showed the lowest activity. The minimum biofilm inhibitory concentration (MBIC) against <i>P. aeruginosa</i> was 25% (w/v) for the <i>F. excelsior</i> and <i>Astragalus-Euphorbia</i> honeys and 50% (w/v) for other honeys. However, no honey sample completely inhibited biofilm formation by <i>S. aureus</i>. This study highlights the promising biological activities of Iranian honey samples and further research may reveal their potential application in medicine, food, pharmaceuticals, and cosmetics.</span></span><br> <span style="font-size:10pt"><span style="font-family:Calibri,sans-serif"><b><span lang="EN" style="font-size:9.0pt"><span style="letter-spacing:-.1pt"></span></span></b></span></span></p> Antibacterial Activity, Anti-biofilm Activity, Antioxidant Activity, Honey 74 82 http://ijmm.ir/browse.php?a_code=A-10-2215-1&slc_lang=en&sid=1 Amir Hosseini-nejad amir.hosseiinii1998@gmail.com 100319475328460031179 0009-0008-3253-4265 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Nayyereh Alimadadi nayyereh_alimadadi@yahoo.com 100319475328460031180 100319475328460031180 Yes Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran