en بیوانفورماتیک میکروبی Microbial Bioinformatics مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><span style="color:#ffffff;"><b><span style="background:#1f497d"><span style="letter-spacing:-.1pt">Background and Aim:</span></span></b></span> <span lang="ZH-CN">Some bacteria, including <i>Staphylococcus</i></span><i> </i><i><span lang="ZH-CN">aureus</span></i> <span lang="ZH-CN">(<i>S. aureus</i>), <i>Pseudomonas aeruginosa </i>(<i>P. aeruginosa</i>),</span> <span lang="ZH-CN">and <i>Streptococcus pyogenes</i></span> <span lang="ZH-CN">(<i>S. pyogenes</i>), contribute to the development of infectious diseases. Bacterial diseases are becoming more common due to the increasing resistance of microorganisms to various antibiotics. The global health community grapples with a major challenge posed by the increasing prevalence of antibiotic resistance. As a result, there&rsquo;s a need for novel therapies to replace ineffective antimicrobial drugs. <i>Streptomyces</i>, known for its abundant production of natural compounds, plays a significant role in the arsenal of antimicrobial agents. <i>Dihomo-&gamma;-linolenic acid</i> (DGLA) shows potential as a novel antibacterial compound derived from <i>Streptomyces actinomycinicus</i> (<i>S. actinomycinicus</i>) PJ85. The study investigated bioinformatically how DGLA impacts the virulence factors of <i>S. aureus</i>, <i>P. aeruginosa</i>, and <i>S. pyogenes</i></span>.<br> <span style="color:#ffffff;"><b><span style="background:#1f497d"><span style="letter-spacing:-.1pt">Materials and Methods:</span></span></b></span> <span lang="ZH-CN">The study examined the interaction of DGLA with virulence factors of the mentioned bacteria using STITCH v5.0 for protein-compound interactions. Functional classification of targeted proteins was done using VICMPred, while VirulentPred 2.0 assessed their virulence properties. BepiPred v.2 was used for B cell epitope analysis, and PSORTb v.3 for subcellular protein localization</span>.<br> <span style="color:#ffffff;"><b><span style="background:#1f497d"><span style="letter-spacing:-.1pt">Results:</span></span></b></span> <span lang="ZH-CN">Based on the analysis results, various virulent proteins from organisms interact with DGLA. For instance, in <i>S. aureus </i>COL, we find epiP. In <i>P. aeruginosa</i>, proteins such as PA5371, tesB, PA3829, PA3741, and PA2168 are involved. Additionally, <i>S. pyogenes</i> features scpA15, SPy_0843, and cepA.&rdquo; Moreover, the characteristics or properties of these proteins were also identified</span>.<br> <span style="color:#ffffff;"><b><span style="background:#1f497d"><span style="letter-spacing:-.1pt">Conclusion:</span></span></b> </span><span lang="ZH-CN">DGLA has a significant impact on virulence factors by interacting with various proteins from <i>S. aureus</i>, <i>P. aeruginosa</i>, and <i>S. pyogenes</i>, as evidenced by bioinformatics analysis</span>.</span></span></p> DGLA, Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus pyogenes, Bioinformatics, Antibiotic Resistance 12 28 http://ijmm.ir/browse.php?a_code=A-10-2688-1&slc_lang=en&sid=1 Nawan Nawan nawan@med.upr.ac.id 100319475328460031142 100319475328460031142 Yes Departement of Microbiology, Faculty of Medicine, University of Palangka Raya, Palangkaraya, Indonesia Septi Handayani septi@med.upr.ac.id 100319475328460031143 100319475328460031143 No Departement of Biochemistry, Faculty of Medicine, University of Palangka Raya, Palangkaraya, Indonesia Agnes Immanuela Toemon agnestoemon@gmail.com 100319475328460031144 100319475328460031144 No Departement of Parasitology, Faculty of Medicine, University of Palangka Raya, Palangkaraya, Indonesia