en باکتری شناسی پزشکی Medical Bacteriology مقاله پژوهشی Original Research Article <p>&nbsp;</p> <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><span style="line-height:150%"><b><span style="line-height:150%"><span new="" roman="" times="">Background and Aims:</span></span></b><span style="line-height:150%"><span new="" roman="" times=""> <i>Pseudomonas aeruginosa </i>(<i>P. aeruginosa</i>) is one of the most common opportunistic bacteria causing healthcare-associated infections (HAIs).</span></span> <span style="line-height:150%"><span new="" roman="" times="">Alginate as an adhesion substance plays role in its binding to inanimate surfaces and protecting against environmental conditions. Biofilm also increases the bacterial resistance to antimicrobials and immune system. The present study was aimed to survey the antimicrobial resistance pattern, biofilm formation, and alginate production in the clinical isolates of <i>P. aeruginosa</i>.</span></span></span><br> <span style="line-height:150%"><b><span style="line-height:150%"><span new="" roman="" times="">Materials and Methods:</span></span></b><span style="line-height:150%"><span new="" roman="" times=""> In this cross-sectional study in a period of one year 90 non-duplicative <i>P. aeruginosa </i>were isolated from the clinical samples. Antimicrobial susceptibility testing was performed by disk diffusion method. Biofilm formation and alginate production were measured by microtiter plate and carbazole assay, respectively. Polymerase chain reaction was conducted for detection of </span></span><i><span style="line-height:150%"><span new="" roman="" times="">pslA, pelA, ppyR, algD, algU </span></span></i><span style="line-height:150%"><span new="" roman="" times="">and<i> algL </i>genes.</span></span></span><br> <span style="line-height:150%"><b><span style="line-height:150%"><span new="" roman="" times="">Results:</span></span></b> <span style="line-height:150%"><span new="" roman="" times="">The highest resistance rate (74.4%) was related to ceftazidime.</span></span> <span style="line-height:150%"><span new="" roman="" times="">Alginate production was found in 87.8% of the isolates, which production levels were <250 &micro;gml^-1, 250-400 &micro;gml^-1, and >400 &micro;gml^-1 in 7.6%, 53.2%, and 39.2 of the isolates, respectively. The categories of biofilm formation in strains were as weak (11.1%), moderate (24.1%), and strong (64.8%). The prevalence of </span></span><i><span style="line-height:150%"><span new="" roman="" times="">ppyR, algD</span></span></i><span style="line-height:150%"><span new="" roman="" times="">, <i>algU, algL, pslA</i> and <i>pelA </i>genes were 100.0%, 92.2%, 86.6%, 67.7%, 85.2% and 42.5%, respectively.</span></span></span><br> <span style="line-height:150%"><b><span style="line-height:150%"><span new="" roman="" times="">Conclusion:</span></span></b> <span style="line-height:150%"><span new="" roman="" times="">The results highlighted an alarming trend in <i>P. aeruginosa</i> strains antibiotic resistance rates. A significant relationship was also observed between the alginate production and the biofilm formation. Thus, periodic monitoring, adherence to the antibiotic stewardship, avoiding arbitrary prescribing, and screening tests are unavoidable.</span></span></span></span></span></p> Alginate, Antibiotic Resistance, Biofilm, PCR, Pseudomonas aeruginosa 319 328 http://ijmm.ir/browse.php?a_code=A-10-68-4&slc_lang=en&sid=1 Maryam Shafigh maryamsh81@gmail.com 100319475328460030734 100319475328460030734 No Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran Rabieh Izadi Amoli Rabi_izadiamoli@yahoo.com 100319475328460030735 100319475328460030735 No Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran Abazar Pournajaf abazar_pournajaf@yahoo.com 100319475328460030736 100319475328460030736 Yes Infectious Disease and Tropical Medicine Research Center, Health Research institute, Babol University of Medical Sciences, Babol, Iran Yousef Yahyapour uyahyapoor@yahoo.com 100319475328460030737 100319475328460030737 No Infectious Disease and Tropical Medicine Research Center, Health Research institute, Babol University of Medical Sciences, Babol, Iran Hami Kaboosi hkaboosi@gmail.com 100319475328460030738 100319475328460030738 No Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran