en ویروس شناسی پزشکی Medical Virology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:200%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Background & Aims:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""> Acute gastroenteritis (AGE) is one of the prevalent factors that threaten human health and is among the main causes of childhood morbidity and fatality rates globally. &nbsp;Viruses are considered one of the main causes of AGE. The current investigation aimed to detect the molecular prevalence of four enteric viruses in AGE. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Methods:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""> One hundred gastrointestinal specimens were obtained from AGE patients from hospitals affiliated with the Iran University of Medical Sciences during 2021-2022. After viral nucleic acid extraction, a real-time Polymerase chain Reaction (Real-time-PCR) was performed to investigate five enteric viruses.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Results:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""> Among 100 patients, interested viruses were diagnosed in 32 (32%) of patients, who </span></span></span><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""><span style="color:black">suffered from various gastrointestinal manifestations such as diarrhea, stomach pain, and vomiting</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">. Norovirus (n=10, 32%) was the most common enteric virus, followed by Rotavirus (n=9, 29%), Bocavirus (n=8, 25%), and Adenovirus (n=5, 14).</span></span></span><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> The most virus-positive patients were males (19/32) including Norovirus (7/10), Rotavirus (5/9), Bocavirus (4/8), and Adenovirus (3/5) samples. </span></span></span></span><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">A high proportion of viruses was detected in children under 12 months,</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""> Our investigation was performed to detect the frequency of different enteric viruses in AGE patients. The finding indicated that Norovirus and Rotavirus are major viral pathogens inducing gastrointestinal infection in patients, respectively. Also, accurate diagnosis of gastrointestinal virus agents can help early treatment and prevent unnecessary prescription of antibiotics and drug resistance development.</span></span></span></span></span></span></span></p> Gastroenteritis, Real-time PCR, Human Rotavirus, Human Norovirus, Human Adenovirus, Human Bocavirus 230 237 http://ijmm.ir/browse.php?a_code=A-10-2338-3&slc_lang=en&sid=1 Zahra Salavatiha z.salavatiha96@gmail.com 100319475328460029893 100319475328460029893 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Ahmad Tavakoli ahmad.tavakoli66@gmail.com 100319475328460029894 100319475328460029894 No Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran Seyed Jalal Kiani kiani.j@iums.ac.ir 100319475328460029895 100319475328460029895 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Mohammad Reza Rezvani mohammadrezarezvani574@gmail.com 100319475328460029896 100319475328460029896 No Department of Hematology and Blood Transfusion, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran Roya Mokarinejad royamokari@gmail.com 100319475328460029897 100319475328460029897 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Seyed Hamidreza Monavari hrmonavari@yahoo.com 100319475328460029898 100319475328460029898 Yes Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran