en میکروب شناسی محیطی - صنعتی Environmental Microbiology مقاله کوتاه پژوهشی Brief Original Article <p>&nbsp;</p> <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><span style="line-height:150%"><span new="" roman="" times=""><b><span lang="EN-US" style="background:yellow"><span style="line-height:150%"><span style="background-color:#ffffff;">Background and Objective:</span></span></span></b><span lang="EN-US" style="background:yellow"><span style="line-height:150%"><span style="background-color:#ffffff;"> Pseudomonas aeruginosa is known for its ability to produce biofilms on medical device surfaces, contributing to resistance against current antibiotic treatments. Ozonated olive oil has demonstrated antibacterial effects, prompting this study to evaluate its efficacy against biofilms produced by two strains of Pseudomonas aeruginosa.</span></span></span></span></span><br> <span style="line-height:150%"><span new="" roman="" times=""><b><span lang="EN-US" style="background:yellow"><span style="line-height:150%"><span style="background-color:#ffffff;">Methods:</span></span></span></b><span lang="EN-US" style="background:yellow"><span style="line-height:150%"><span style="background-color:#ffffff;"> This study investigated the potential of ozonated olive oil to prevent biofilm formation, degrade established biofilms, and penetrate biofilms after ozone treatment.</span></span></span></span></span><br> <span style="line-height:150%"><span new="" roman="" times=""><b><span lang="EN-US" style="background:yellow"><span style="line-height:150%"><span style="background-color:#ffffff;">Results & Conclusion:</span></span></span></b><span lang="EN-US" style="background:yellow"><span style="line-height:150%"><span style="background-color:#ffffff;"> Both 5% and 10% ozonated olive oil inhibited biofilm formation in the P. aeruginosa POA1 strain, while the 1707 strain showed resistance to all concentrations tested. Treatment with 15% ozonated olive oil resulted in the complete elimination of the PAO1 strain biofilm. Additionally, 5% ozonated olive oil effectively removed the 1707 biofilm structure. All concentrations (10%, 15%, and 30%) penetrated the PAO1 biofilm and eliminated sessile bacteria, whereas only 5% and 15% ozonated olive oil could penetrate the biofilm of the 1707 strain. These findings suggest that ozonated olive oil exhibits significant anti-biofilm effects against Pseudomonas aeruginosa.</span></span></span></span></span></span></span></p> Biofilm,Pseudomonas aeruginosa,Ozonated olive oil,Antibacterial effect. 329 336 http://ijmm.ir/browse.php?a_code=A-10-2486-1&slc_lang=en&sid=1 Saba Dadpour saba.dp@gmail.com 100319475328460030739 100319475328460030739 No Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran Zahra Sabeti Noghabi sabetinz1@mums.ac.ir 100319475328460030740 100319475328460030740 No Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran Atoosa Haghighizadeh atoosa.hz@gmail.com 100319475328460030741 100319475328460030741 No Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran Leila Etemad etemadl@mums.ac.ir 100319475328460030742 100319475328460030742 No Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran Vahid Soheili soheiliv@mums.ac.ir 100319475328460030743 100319475328460030743 No Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran Omid Rajabi rajabio@mums.ac.ir 100319475328460030744 100319475328460030744 Yes Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran