fa ﺑﺮرﺳﯽ اﺛﺮ ﺿﺪ ﻣﯿﮑﺮوﺑﯽ ﮐﻔﯿﺮ در زﻣﺎﻧﻬﺎی ﻣﺘﻔﺎوت ﺗﺨﻤﯿﺮ‬ باکتری شناسی پزشکی Medical Bacteriology مقاله پژوهشی Original Research Article <div align="justify" style="direction: rtl"> زمینه و ﻫﺪف: ﮐﻔﯿﺮ ﯾﮏ ﭘﺮوﺑﯿﻮﺗﯿﮏ ﮐﻤﭙﻠﮑﺲ ﻣﯽ ﺑﺎﺷﺪ ﮐﻪ ﻣﺒﺪأ آن ﺷﻤﺎل ﻗﻔﻘﺎز اﺳﺖ. ﺑﻄﻮر ﮐﻠﯽ داﻧﻪ ﻫﺎی ﮐﻔﯿﺮ دارای‬ ‫ﻣﯿﮑﺮوارﮔﺎﻧﯿﺴﻢ ﻫﺎﯾﯽ ﻧﻈﯿﺮ اﺳﺘﺮﭘﺘﻮﮐﻮﮐﺴﯽ ﻫﺎی اﺳﯿﺪ ﻻﮐﺘﯿﮏ، ﻻﮐﺘﻮﺑﺎﺳﯿﻞ ﻫﺎی ﻣﺰوﻓﯿﻠﯿﻠﯿﮏ اﺳﯿﺪ ﻻﮐﺘﯿﮏ، ﻣﺨﻤﺮﻫﺎی‬ ‫ﺗﺨﻤﯿﺮ ﮐﻨﻨﺪه و ﻏﯿﺮ ﺗﺨﻤﯿﺮ ﮐﻨﻨﺪه ﻻﮐﺘﻮز و ﺑﺎﮐﺘﺮﯾﻬﺎی اﺳﯿﺪ اﺳﺘﯿﮏ اﺳﺖ، ﮐﻪ ﺑﻪ اﺛﺮات درﻣﺎﻧﯽ آﻧﻬﺎ اﺷﺎره ﺷﺪه اﺳﺖ.‬ ‫<br><b>ﻣﻮاد و روﺷﻬﺎ:</b> در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺗﺠﺮﺑﯽ اﺛﺮات ﺿﺪ ﻣﯿﮑﺮوﺑﯽ ﮐﻔﯿﺮ ﺑﺮ روی ﺑﺎﮐﺘﺮی <i>ﺳﻮدوﻣﻮﻧﺎس آﺋﺮوژﻧﻮزا</i> ﺟﺪا ﺷﺪه از‬ ‫ﺑﯿﻤﺎر دﭼﺎر ﺳﻮﺧﺘﮕﯽ و <i>ﺳﻮدوﻣﻮﻧﺎس آﺋﺮوژﻧﻮزا</i> اﺳﺘﺎﻧﺪارد  (ATCC 27853‬) ﺑﺎ اﺳﺘﻔﺎده از ﻣﺤﯿﻄﻬﺎی ﮐﺸﺖ‬ ‫ﻣﯿﮑﺮوﺑﯿﻮﻟﻮژی و ﺗﻌﯿﯿﻦ‪ MIC ‬ﺑﺎ اﺳﺘﻔﺎده از روش ﻫﺎی رﻗﺖ ﻟﻮﻟﻪ ای و روش ﭼﺎﻫﮏ و ﺑﺮرﺳﯽ ﮐﯿﻨﯿﺘﯿﮏ ﻣﺮگ در‬ ‫ﺣﻀﻮر دو ﻧﻮع از ﻋﺼﺎره ﻫﺎی ﮐﻔﯿﺮ در دو زﻣﺎن ﻣﺘﻔﺎوت ﺗﺨﻤﯿﺮ 96 و 72 ﺳﺎﻋﺖ در دﻣﺎی 35 درﺟﻪ ﺳﻠﺴﯿﻮس ﺑﻪ‬ ‫ﺻﻮرت ‪ in vitro‬ ﻣﻮرد ﻣﻄﺎﻟﻌﻪ ﻗﺮار ﮔﺮﻓﺖ. ﻣﯿﺰان اﺳﯿﺪ ﻻﮐﺘﯿﮏ و اﺳﯿﺪ اﺳﺘﯿﮏ ﻧﯿﺰ ﺑﺎ ‪  HPLC‬ﻓﺎز ﻣﻌﮑﻮس اﻧﺪازه‬ ‫ﮔﯿﺮی ﮔﺮدﯾﺪ.‬ <br>‫<b>ﯾﺎﻓﺘﻪ ﻫﺎ:</b> در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺗﺠﺮﺑﯽ، ﺑﯿﺸﺘﺮﯾﻦ اﺛﺮ ﺿﺪ ﻣﯿﮑﺮوﺑﯽ ﻋﺼﺎره ﮐﻔﯿﺮ در دﻣﺎی 35درﺟﻪ ﺳﻠﺴﯿﻮس ﺑﺎ 96 ﺳﺎﻋﺖ‬ ‫زﻣﺎن ﺗﺨﻤﯿﺮ و ﻏﻠﻈﺖ 250 ﻣﯿﻠﯽ ﮔﺮم در ﻣﯿﻠﯽ ﻟﯿﺘﺮ روی <i>ﺳﻮدوﻣﻮﻧﺎس آﺋﺮوژﻧﻮزا</i> (ATCC 27853‬) و‬ <i> ‫ﺳﻮدوﻣﻮﻧﺎس آﺋﺮوژﻧﻮزا</i> ﺟﺪا ﺷﺪه از ﺑﯿﻤﺎر ﺳﻮﺧﺘﻪ ﺑﺪﺳﺖ آﻣﺪ.‬ <br> ‫<b>ﻧﺘﯿﺠﻪ ﮔﯿﺮی:</b> ﻋﺼﺎره ﮐﻔﯿﺮ دارای اﺛﺮات ﺿﺪ ﻣﯿﮑﺮوﺑﯽ ﺑﺮ روی ﺑﺎﮐﺘﺮی <i>ﺳﻮدوﻣﻮﻧﺎس آﺋﺮوژﻧﻮزا</i>(ATCC 27853‬) اﺳﺘﺎﻧﺪارد‬ و <i>ﺳﻮدوﻣﻮﻧﺎس آﺋﺮوژﻧﻮزا</i> ﺟﺪا ﺷﺪه از ﺑﯿﻤﺎر ﺳﻮﺧﺘﻪ ﻣﯽ ﺑﺎﺷﺪ.‬ </div> <b>Introduction:</b> Kefir is a probiotic mixture of bacteria and yeast originating from Qafqaz region.The Kefir grain contain both Lactic acid bacteria (Lactobacillus, Lactococcus, Leuconostoc, Acetobacte and Streptococcus spp.) and yeast (<i>Kluyveromyces,Torula</i>, Candida and <i>Saccharomyces spp</i>.). Kefir is claimed to have therapeutic effect.This study looked at the antimicrobial activity of Kefir on <i>Pseudomonas aeruginosa</i>. <br><b>Methods: </b> The antimicrobial activity of Kefir extract were determined on <i>Pseudomonas aeruginosa </i> (ATCC 27853) and on those isolated from burned patient. The MIC was defined as the lowest antimicrobial concentration able to completely inhibite bacterial growth up to 24 h. MIC values were determined by microdilution method. The lactic acid and acetic acid contents of the Kefir extracts were determined by reverce-phase HPLC. <br><b>Result: </b> The result showed that the highset antimicrobial activity of Kefir exteract on <i>Pseudomonas aeruginosa</i> (ATCC 27853) and the burned patient isolate ranged from 250 mg/mL(MIC) to 250 mg/mL(MBC) on time 96h. <br><b>conclusion:</b> The Kefir exteract showed significant antimicrobial activity on <i> Pseudomonas aeruginosa</i> (ATCC 27853) and the clinical isolate of <i>Pseudomonas aeruginosa.</i> اﺛﺮات ﺿﺪ ﻣﯿﮑﺮوﺑﯽ، ﮐﻔﯿﺮ، ﺗﺨﻤﯿﺮ‬ Kefir, Antimicrobial activity, fermentation. 35 41 http://ijmm.ir/browse.php?a_code=A-10-1-167&slc_lang=fa&sid=1 Golnar Rahimzadeh ‫ﮔﻠﻨﺎر رﺣﯿﻢ زاده rahimzadehgolnar@yahoo.com 10031947532846001028 10031947532846001028 Yes Department of Biology, Islamic Azad University, Science and Research branch , Tehran, Ira ﮔﺮوه زﯾﺴﺖ ﺷﻨﺎﺳﯽ داﻧﺸﮑﺪه ﻋﻠﻮم ﭘﺎﯾﻪ داﻧﺸﮕﺎه آزاد اﺳﻼﻣﯽ واﺣﺪ ﻋﻠﻮم ﺗﺤﻘﯿﻘﺎت ﺗﻬﺮان اﯾﺮان Mohammad Ali Bahar ﻣﺤﻤﺪ ﻋﻠﯽ ﺑﻬﺎر mobahar@yahoo.com 10031947532846001029 10031947532846001029 No Department of Biology, Islamic Azad University, Science and Research branch , Tehran, Ira ﮔﺮوه اﯾﻤﻮﻧﻮﻟﻮژی داﻧﺸﮕﺎه ﻋﻮم ﭘﺰﺷﮑﯽ اﯾﺮان Nour Amir Mozaffari ﻧﻮر اﻣﯿﺮ ﻣﻈﻔﺮی amirmozafari@yahoo.com 10031947532846001030 10031947532846001030 No ﮔﺮوه ﻣﯿﮑﺮوب ﺷﻨﺎﺳﯽ داﻧﺸﮕﺎه ﻋﻮم ﭘﺰﺷﮑﯽ اﯾﺮان