AU - Ghaffari, Mansoureh AU - Noorbakhsh, Fatemeh AU - Kachuei, Reza TI - Study of Lipase genes expression (Lip2,3,5) in Candida albicans isolated from patients PT - JOURNAL ARTICLE TA - Iran-J-Med-Microbiol JN - Iran-J-Med-Microbiol VO - 11 VI - 2 IP - 2 4099 - http://ijmm.ir/article-1-672-en.html 4100 - http://ijmm.ir/article-1-672-en.pdf SO - Iran-J-Med-Microbiol 2 ABĀ  - Background and Aims: Candida, is a fungal flora in human that can be colonized on the skin and mucosal surfaces. Various factors are involved in causing disease by candida, one of the most important of these factors, are secreted hydrolytic enzymes and aspartyl proteinase enzymes are as a potential factor, but other secretion of hydrolytic enzymes such as phospholipase and lipase are also important pathogen factors. In this study, gene expression of LIP2, LIP3 and LIP5 were studied in isolated C. albicans in 100 samples from patients. Materials and Methods: A total of 100 strains of C. albicans was isolated in 2015-2016 years by morphology and molecular methods, from clinical samples. After extracting RNA from them by RNX-Plus kit, expression of LIP2, LIP3 and LIP5genes was evaluated by using RT-PCR test. Results: From 100 clinical isolates of C. albicans, LIP2 gene expressed 55%, LIP3 gene 41% and LIP5 gene 48%. Among the positive samples, 24 cases (33.80%) were expressed three genes at the same time. Conclusions: In this study, frequency of lipase genes was different in strains of C.albicans. Lipase gene was not expressed in 30% of cases. It can be concluded that other genes than lipase gene can be involved in the pathogenesis. CP - IRAN IN - Department of Microbiology, Biological Science College, Varamin-pishva branch, Islamic Azad University,Varamin-Pishva, Iran LG - eng PB - Iran-J-Med-Microbiol PG - 53 PT - Original Research Article YR - 2017