year 13, Issue 5 (November - December 2019)                   Iran J Med Microbiol 2019, 13(5): 355-373 | Back to browse issues page


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Ranjbar R, Roayaei Ardakani M, Mehrabi Kushki M, kazeminezhad I. Identification of Toxigenic Aspergillus Species from Rice of Khuzestan and Mycotoxins in Imported Cereals. Iran J Med Microbiol 2019; 13 (5) :355-373
URL: http://ijmm.ir/article-1-971-en.html
1- Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2- Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran , roayaei_m@yahoo.com
3- Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4- Department of Physic, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Abstract:   (5200 Views)
Background: Due to their high amount of carbohydrate and enough moisture, cereals are a good environment for the growth of toxigenic fungi. Because of the carcinogenicity and mutagenicity of mycotoxins, preventing them from entering the food chain is essential. Therefore, the present study was conducted to determine the amount and type of contaminated imported cereals and rice produced in Khuzestan province
Materials & Methods: In October and November 2015, a total of 50 random samples of rice was collected from paddy fields. Aspergillus were identified based on available diagnostic criteria and PCR. The amount and type of aflatoxin in rice samples and mycotoxins in imported cereals (winter 2015 to autumn 2016) were evaluated by HPLC
Result: Based on one sample t-test and comparing the mean of mycotoxins contaminating cereals in different seasons with national maximum standard, the amount of mycotoxins in barley and wheat were within the standard range but %8.4 of corn was higher than the permitted level (ppb5). Analysis of aflatoxins in rice also showed that 16 samples were contaminated with aflatoxin B1.  Aspergillus flavus was the major pollutant (%42.1) isolated from rice.
 Conclusion: Aspergillus flavus is the major producer of aflatoxin B1 in domestic rice. Examination of imported cereals also showed high rates of fungal growth and production of secondary metabolites, possibly due to inadequate storage conditions, high temperature and humidity.   Therefore, it is recommended to strengthen the monitoring tools in the processing and storage of rice and cereals.
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Type of Study: Original Research Article | Subject: Medical Mycology
Received: 2019/10/8 | Accepted: 2020/02/6 | ePublished: 2020/02/11

References
1. Pleadin J, Vulic A, Persi N, Skrivanko M, Capek B, Cvetni Z. Aflatoxin B1 occurrence in maize sampled from Croatian farms and feed factories during 2013. Food Control. 2014; 40: 286-291 [DOI:10.1016/j.foodcont.2013.12.022]
2. Leslie, J. F., Bandyopadhyay, R. and Visconti, A.(Eds.). Mycotoxins: detection methods, management, public health and agricultural trade. 2008;CABI [DOI:10.1079/9781845930820.0000]
3. Alberts, J. F., Lilly, M., Rheeder, J. P., Burger, H. M., Shephard, G. S. and Gelderblom, W. C. A. Technological and community-based methods to reduce mycotoxin exposure. Food Control. 2017; 73: 101-109 [DOI:10.1016/j.foodcont.2016.05.029]
4. Abbas, H. K., Wilkinson, J. R., Zablotowicz, R. M., Accinelli, C., Abel, C. A., Bruns, H. A. and Weaver, M. A. Ecology of Aspergillus flavus, regulation of aflatoxin production, and management strategies to reduce aflatoxin contamination of corn. Toxin Reviews. 2009; 28(2-3): 142-153. [DOI:10.1080/15569540903081590]
5. Magnussen A, Parsi M.A. Aflatoxins, hepatocellular carcinoma and public health. World J Gastroenterol. 2013; 19(10): 1508. [DOI:10.3748/wjg.v19.i10.1508] [PMID] [PMCID]
6. Jinap S, De Rijk TC, Arzandeh S, Kleijnen HC, Zomer P, Van der Weg G, Mol JG. Aflatoxin determination using in-line immunoaffinity chromatography in foods. Food Control. 2012; 26(1): 42-48. [DOI:10.1016/j.foodcont.2011.12.007]
7. Pitt, J. I.: Hocking, A. D. Fungi and food spoilage. New York: Springer; 2009; 519. P. 275-33. [DOI:10.1007/978-0-387-92207-2_8]
8. Samson, R. A., Visagie, C. M., Houbraken, J., Hong, S. B., Hubka, V., Klaassen, C. H. and Varga, J. Phylogeny, identification and nomenclature of the genus Aspergillus. Studies in Mycology. 2014; 78: 141-173. [DOI:10.1016/j.simyco.2014.07.004] [PMID] [PMCID]
9. Samson RA, Noonim P, Meijer M, Houbraken JA, Frisvad JC, Varga J. Diagnostic tools to identify black aspergilli. Studies in mycology. 2007;59:129-45. [DOI:10.3114/sim.2007.59.13] [PMID] [PMCID]
10. Gupta VK (Ed). New and future developments in microbial biotechnology and bioengineering: Aspergillus system properties and applications. Elsevier; 2016.
11. Mehrabi-Koushki, M. Bavarsad, M., Farrokhinejad, R., Jamshidi, J. and Alimohammadi, A. The comparison of ITS-rDNA and tef1α genomic regions for phylogenetic study of some Trichoderma Species. Iranian Journal of Plant Protection Science.2016; 47, 61-70. [ In Persian]
12. Raeder U. and Broda P. Rapid preparation of DNA from filamentous fungi. Letters in Applied Microbiology. 1985;1: 17-20. [DOI:10.1111/j.1472-765X.1985.tb01479.x]
13. Nyongesa, B. W., Okoth, S. and Ayugi, V. Identification Key for Aspergillus Species Isolated from Maize and Soil of Nandi County, Kenya. Advances in Microbiology. 2015; 5(04): 205-229 [DOI:10.4236/aim.2015.54020]
14. Riba A, Bouras N, Mokrane S, Mathieu F, Lebrihi A, Sabaou N. Aspergillus section Flavi and aflatoxins in Algerian wheat and derived products. Food and Chemical Toxicology. 2010; 48(10): 2772-2777 [DOI:10.1016/j.fct.2010.07.005] [PMID]
15. Gao, J., Liu, Z. and Yu, J. Identification of Aspergillus section Flavi in maize in northeastern China. Mycopathologia. 2007;164(2): 91-95. [DOI:10.1007/s11046-007-9029-4] [PMID]
16. Makun, H. A., Gbodi, T. A., Akanya, O. H., Salako, E. A. and Ogbadu, G. H. Fungi and some mycotoxins contaminating rice (Oryza sativa) in Niger state, Nigeria. African Journal of Biotechnology. (2007);6(2):99-108
17. Amadi, J. E. and Adeniyi, D. O. Mycotoxin production by fungi isolated from stored grains. African Journal of Biotechnology. 2009; 8(7):1219-1221
18. Magnussen A, Parsi M.A. Aflatoxins, hepatocellular carcinoma and public health. World J Gastroenterol. 2013; 19(10): 1508. [DOI:10.3748/wjg.v19.i10.1508] [PMID] [PMCID]
19. Hassan ZU, Al-Thani RF, Migheli Q, Jaoua S. Detection of toxigenic mycobiota and mycotoxins in cereal feed market. Food Control. 2018; 84: 389-394. [DOI:10.1016/j.foodcont.2017.08.032]
20. 20- Reiter E.V, Vouk F, Bohm J, Razzazi-Fazeli E. Aflatoxins in rice - A limited survey of products marketed in Austria. Food Control. 2010; 21(7): 988-991. [DOI:10.1016/j.foodcont.2009.12.014]
21. Ghali, R., Khlifa, K. H., Ghorbel, H., Maaroufi, K. and Hedilli, A. Aflatoxin determination in commonly consumed foods in Tunisia. J Sci Food Agric. 2010; 90(14): 2347-2351. [DOI:10.1002/jsfa.4069] [PMID]
22. Mazaheri M, MahmoudiMaymand M. Investigation of Aflatoxins in Imported Animal Feeds in Iran. Iranian Journal of Toxicology. 2018;12(6):33-8.
23. Amanloo S, Rezaei Kahhka MR, Ramezani AA, Mir L (2014) .The Mycotoxin contamination of the imported consumer rice and its producing fungi in Zabol . Journal of Jahrom University of Medical Sciences. 12(1): 17-25. [In Persian] [DOI:10.29252/jmj.12.1.17]
24. Farahmandfar R, Rashidaei Abandansari S, Maghsoudlou E, Asnaashari M. Determination of mycotoxin contamination in imported wheat to Mazandaran province by high performance liquid chromatography. Iranian Journal of Health and Environment. 2018;11(1):15-24.
25. Najafian M. Comparison the level of Aflatoxin in different varieties of internal and imported rice in different collection seasons and effect of cooking methods on the level of toxins. 2014; 6(4): 328-336. [In Persian]
26. Ranjbar S, Noori M, Nazari R. Study of milk aflatoxin M1 and its relationship with feed fungi flora in Markazi Province. J Cell Tissue. 2010; 1(1): 9-18. [In Persian]
27. Cano-Sancho G, Sanchis V, Marin S, Ramos A.J. Occurrence and exposure assessment of aflatoxins in Catalonia (Spain). Food and Chemical Toxicology. 2013; 51: 188-193. [DOI:10.1016/j.fct.2012.09.032] [PMID]
28. Lahouar, A., Marin, S., Crespo-Sempere, A., Saïd, S. and Sanchis, V. Effects of temperature, water activity and incubation time on fungal growth and aflatoxin B1 production by toxinogenic Aspergillus flavus isolates on sorghum seeds. Revista Argentina de Microbiología. 2016; 48(1): 78-85. [DOI:10.1016/j.ram.2015.10.001] [PMID]

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