Biofilm Formation of Salmonella typhimurium on Stainless Steel in Red Meat Model and the Effect of Bacteriophage on Bacterial Biofilm

Tajik, Hossein; Moradi, Mehran; Alipour, Mosatafa; Ghasemmahdi, Hadi

doi:10.30699/ijmm.12.3.179

year 12, Issue 3 (July - August 2018) Iran J Med Microbiol 2018, 12(3): 179-188 | Back to browse issues page

‎ 10.30699/ijmm.12.3.179

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Tajik H, Moradi M, Alipour M, Ghasemmahdi H. Biofilm Formation of Salmonella typhimurium on Stainless Steel in Red Meat Model and the Effect of Bacteriophage on Bacterial Biofilm. Iran J Med Microbiol 2018; 12 (3) :179-188
URL: http://ijmm.ir/article-1-690-en.html

Biofilm Formation of Salmonella typhimurium on Stainless Steel in Red Meat Model and the Effect of Bacteriophage on Bacterial Biofilm

Hossein Tajik¹

, Mehran Moradi

², Mosatafa Alipour¹

, Hadi Ghasemmahdi¹

1- Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
2- Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran , moradi.mehran@yahoo.com

Abstract: (6049 Views)

Background and Aims: The application of bacteriophage to control and removal of bacterial biofilm is a novel method. The goal of this study was to investigate the effect of the Salmonella typhimurium bacteriophage against biofilm of a multidrug resistance (MDR) Salmonella formed on stainless steel in beef broth.
Materials and Methods: One and 7 days old biofilm were grown at 15, 8 and 4 °C, on the stainless steel in beef broth and the effects of different bacteriophage concentrations (10³, 10⁵ and 10⁷ PFU/mL) with two contact times (10 and 15 min) were assayed.
Results: Results showed that Salmonella can adhere to stainless steel and form biofilm in the beef broth which was significantly influenced by temperature. Higher biomass of biofilm was developed at 15, 4 and 8 °C, respectively. One-day-old is less dense (~1 logarithmic cycle) than 7-day-old biofilm. No significant difference (P>0.05) in biofilm reduction was observed in samples treated with different concentration as compared with control. Statistical differences were also not observed in the different contact time (10 and 15 min).
Conclusions: These results indicated that there was no significant reduction in MDR Salmonella biofilm population developed on stainless steel in the beef broth after using the bacteriophage; it is needed to investigate some combination procedures or increase contact time to improve the biofilm removal activity of bacteriophage.