year 11, Issue 3 (July - August 2017)                   Iran J Med Microbiol 2017, 11(3): 10-18 | Back to browse issues page

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Rajaei S, Kazemi-Pour N, Rokhbakhsh-Zamin F. Frequency of Plasmid-Mediated Quinolone Resistance Genes among Clinical Isolates of Pseudomonas aeruginosa in Kerman, Iran. Iran J Med Microbiol. 2017; 11 (3) :10-18
URL: http://ijmm.ir/article-1-683-en.html
1- Department of Microbiology, Islamic Azad University, Kerman, Iran
2- Department of Microbiology, Islamic Azad University, Kerman, Iran , nadia_kazemi@yahoo.com
Abstract:   (8029 Views)

Background and Aims: plasmid genes are responsible for resistance to quinolones and fluoroquinolones, so the present study aims to identify the presence of qnr and aac genes in clinical isolates of P. aeruginosa.
Materials and Methods: In 2016, 60 strains of P. aeruginosa were isolated from clinical specimens of patients in Kerman hospitals. Antibiotic resistance patterns were evaluated  using Kirby-Bauer and microdilution methodsagainst 10 antibiotics according to CLSI criteria Specific primers and polymerase chain reaction were used to detect and amplify qnr and aac(6)-Ib-cr genes.
Results: Maximum antibiotic resistance was observed against cefexim (80%) and calidixic acid (75%) and minimum resistance against imipenem (25%) and ciprofloxacin (35%). The incidence rate of quinolone resistance genes were as follows: qnr A (16.66%), qnrB (13.33%), qnrS (11/66%) and aac(6)-Ib-cr (8/33%).
Conclusions: In the present study, qnrA gene had the highest incidence rates among the studied genes. Because of the importance of antibiotic resistance and the high prevalence of qnr genes found in this study , furthur studies need to be carried out on qnr resistance genes in the regional and national dimension.
 

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Type of Study: Original | Subject: Antibiotic Resistance
Received: 2017/03/15 | Accepted: 2017/06/17 | ePublished: 2017/08/8

References
1. Mohamadbigi M, Akbarmehr J, Jafari B. Evaluation of frequency of Plasmid-mediated quinolone resistance genes in clinical isolates of Escherichia coli and Klebsiella spp in Tehran: J Microbial World (JMW) 2016; 9(3):199-207. [in Persian]
2. Amini B, Kamali M, Zarei Mahmoud Abadi A, Bayat A, Javadi H, Mansori M,, et al. Isolation and rapid identification of Pseudomonas aeruginosa by PCR, Quarterly J Biological Sci, Islamic Azad Univ, Zanjan. 2009; 3(1):6-59. [in Persian]
3. Dashtizadeh Y, Moattari A, Gorzin A. Phenotypic and genetically evaluation of the prevalence of efflux pumpsand antibiotic resistance in clinical isolates of Pseudomonasaeruginosa among burned patients admitted to Ghotbodin Shirazi Hospital, Journal of Microbial World (JMW) 2014; 7(2):118-127. [in Persian]
4. Sepehrisaresht S, Najarpyrayeh Sh, Sattari M, Ahangarzadeh M, Rezaei B. Evaluation of plasmid mediated Beta Lactamase production in pseudomonas aeruginosa strains isolated from burns, Hakim Res J. 2007; 10 (1). [in Persian]
5. Moghbeli M, Behnood V, Ranjbar R. A study to determine antibiotic resistance and recognition qnr genesin Shigella strains isolated from patients admitted to Mofid's Children Medical Center, Tehran, J Microbial World (JMW) 2014; 7(1):49-57. [in Persian]
6. Mardaneh J, Ahmadi Kh, Jahansepas A. Antibiotic resistance profiles of Pseudomonas aeruginosa strains isolated from patients at Taleghani hospital in Ahvaz during 2011- 2012, J Fasa Univ Med Sci (GFUMS) 2013; 3. [in Persian]
7. Salehi M, Hekmatdoost M, Hosseini F. Quinolone resistance associated with mexAB-oprM secretory pump from Pseudomonas aeruginosa isolates, J Microbial world (JMW) 2013; 4 (17): 290-298. [in Persian]
8. Cayci YT, Coban AY, Gunaydin M. Investigation of plasmid-mediated quinolone resistance in Pseudomonas aeruginosa clinical isolates. Indian J Med Microbiol 2014 Jul-Sep;32(3):285-9.
9. Cattoir V, Nordmann P. Plasmid-mediated quinolone resistance in gram negative bacterial species: An update. Curr Med Chem 2009;16:1028-46.
10. Soleimani-asl Y, Zibaei M, Firoozeh F. Detection of qnrA gene quinolone-resistant Escherichia coli isolated from urinary tract infections in Khorram Abad during 2011-2012. Feyz 2013;17(5): 488-494. [in Persian]
11. Al- Marjani M. Presence of qnr gen in Environmental and Clinical pseudomonas aeruginosa Isolates in Baghdad. International J Microbiology and Applied sci Issn 2014; 3 (7): 853-857.
12. Saleh M, Balboula M. Plasmid Mediated Quinolone Resistance Determinants among Nosocominal Chinical pseudomonas12 aeruginosa Isolates. International J Microbiology and Applied sci Issn 2017; 6 (1): 42-50.
13. Cayci YT,Coban AY,Gunaydin M. Investigation of plasmid-mediated quinolone resistance in Pseudomonas aeruginosa clinical isolates. Indian J Med Microbiol. 2014; 32(3):285-9.
14. Deyhim B, Bessikhasteh M. The pattern of antibiotic resistance and MBL production of Pseudomonas aeruginosa strains isolated from clinical samples from Dr. Ganjavian hospital, Islamic Azad University of Dezful. J Microbial Biotechnology 2012; 4 (4): 21-28. [in Persian]
15. Anvarinezhad M, japoni A, Rafatpour N, Alipour A, Abbasi P, Manelyshahidi M, et al. Antimicrobial susceptibility patterns and molecular epidemiology of Pseudomonas aeruginosa isolated from burn patients. Armaghane Danesh, Scientific and research J med scie Yasooj Univ. 2014; 19 (8): 91. [in Persian]

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