Investigation and Optimization Effects of ultrasound waves to produce Ganoderic acid, anti-cancer mushrooms metabolite

Nojoki, Fahimeh; Hatamian-Zarmi, Ashrafalsadat; Ebrahimi-Hosseinzadeh, Bahman; Mir-drikvand, Mohammad; Mokhtari-Hosseini, Zahra begom; Kalantari-Deahaghi, Saeid

year 11, Issue 1 (March - April 2017) Iran J Med Microbiol 2017, 11(1): 58-66 | Back to browse issues page

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Nojoki F, Hatamian-Zarmi A, Ebrahimi-Hosseinzadeh B, Mir-drikvand M, Mokhtari-Hosseini Z B, Kalantari-Deahaghi S. Investigation and Optimization Effects of ultrasound waves to produce Ganoderic acid, anti-cancer mushrooms metabolite. Iran J Med Microbiol 2017; 11 (1) :58-66
URL: http://ijmm.ir/article-1-572-en.html

Investigation and Optimization Effects of ultrasound waves to produce Ganoderic acid, anti-cancer mushrooms metabolite

Fahimeh Nojoki¹

, Ashrafalsadat Hatamian-Zarmi

², Bahman Ebrahimi-Hosseinzadeh¹

, Mohammad Mir-drikvand¹

, Zahra begom Mokhtari-Hosseini³

, Saeid Kalantari-Deahaghi¹

1- Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
2- Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran , hatamian_a@ut.ac.ir
3- Department of Chemical Engineering, Faculty of Petroleum and Petrochemical Engineering, Hakim Sabzevari University, Sabzevar, Iran

Abstract: (8319 Views)

Background and Aim: Ganoderma lucidum is a main source of ganoderic acid. This metabolite has pharmaceutical effects such as cancer treatment. However, due to low yields and high prices, clinical application is difficult. Stimulants such as elicitor and mechanical shocks can be used to increase the produce of ganoderic acid effectively. This study aimed to investigate the effect of ultrasound on the production of this medicine.

Materials and Methods: In this study, a liquid culture of the fungus treated with the once and twice at 20 and 200 seconds of sound with a frequency of 50 kHz and constant output power in ultrasonic bath was investigated. After extraction of ganoderic acid and ensure of positive impact of shock, to optimize variables, response surface methodology was used. In this method, 17 experiments with three variables, the first time ultrasound (4, 5, 6 days), the second ultrasound (7, 8, 9 days) and time of ultrasound (60, 180, 300 seconds) by Design Expert software were designed and done.

Results: The results showed that the effects of ultrasound stimulation with twice 274 seconds treatment on days 4 and 7 were established highest ganoderic acid production. By comparing samples with control (without sound) Increase in extracellular ganoderic acid to intracellular ratio was determined. In optimum conditions, 34% increase in GA production relative to the control sample was observed.

Conclusions: Ultrasound can be used as a non-chemical and cheap stimulus, increase ganoderic acid production.

Keywords: Ultrasound, Ganoderma lucidum, Ganoderic acid, Anticancer metabolite

Full-Text [PDF 911 kb] (2930 Downloads)

Type of Study: Original Research Article | Subject: Microbial Biotechnology
Received: 2016/06/3 | Accepted: 2016/09/30 | ePublished: 2016/10/17

References

1. Wu GS, Lu J, Guo JJ, Li YB, Tan W. Ganoderic acid DM a natural triterpenoid induces DNA damage G1 cell cycle arrest and apoptosis in human breast cancer cells. Fitoterapia 2012; 83(2):408-14. [PubMed]

2. Eo SK, Kim Y, Lee CK, Han S. Antiherpetic activities of various protein bound polysaccharides isolated from Ganoderma lucidum. J Ethnopharmacol 1999; 68(3):175-81. [PubMed]

3. Johnson B, Doonan B, Radwan F, Haque A. Ganoderic acid DM an alternative agent for the treatment of advanced prostate cancer. Open Prost Cancer J 2010; 3(1): 78- 85. [PubMed]

4. Sliva D. Ganoderma lucidum in cancer research. Leuk Res 2006; 30(7):767-8. [Article] [PubMed]

5. Li Y, Liu R, Zhong J. a new ganoderic acid from Ganoderma lucidum mycelia and its stability. Fitoterapia 2013; 84(1):115-22. [PubMed]

6. Xu P, Ding Z, Qian Z, Zhao C, Zhang C. Improved production of mycelial biomass and ganoderic acid by submerged culture of Ganoderma lucidum SB97 using complex media. Enzyme Microb Technol 2008; 42(4):325–31. [Article]

7. Shi L, Ren A, Mu D, Zhao M. current progress in the study on biosynthesis and regulation of ganoderic acids. Microbiol Biotechno 2010; 88(6):1243-51. [PubMed]

8. Zhang J, Zhong J, Geng A. Improvement of ganoderic acid production by fermentation of Ganoderma lucidum with cellulase as an elicitor. Process Biochem 2014; 49(10):1580- 6. [Article]

9. Heydarian MS, Hatamian AS, Amoabediny G, Yazdian F, Doryab A. Synergistic Effect of Elicitors in Enhancement of Ganoderic Acid Production: Optimization and Gene Expression Studies. Appl Food Biotechnol 2015; 2(3):57-62. [Article]

10. Lin L, Wu J, Ho K. Ultrasound-induced physiological effects and secondary metabolite (saponin) production in Panax ginseng cell cultures. Ultrasound Med Bio 2001; 27(8):1147-52. [PubMed]

11. Fang Q, Zhong J. Two-stage culture process for improved production of ganoderic acid by liquid fermentation of higher fungus Ganoderma lucidum. Biotechnol Prog 2002; 18(1):51-4. [PubMed]

12. Qiang G, Hua-Xi X, Xiao H, Wang X, Zhao Y, Zhang Y, Ren G. Stimulated Production of Triterpenoids of Ganoderma lucidum by an Ether Extract from the Medicinal Insect, Catharsius molossus, and Identification of the Key Stimulating Active Components. Appl Biochem Biotechnol 2011; 165(1):87-97. [PubMed]

13. Ziegenbein F, Hanssen H, Konig K. Secondary metabolites from Ganoderma lucidum and Spongiporus leucomallellus. Phytochemistry 2006; 67(2):202-11. [PubMed]

14. Zhao J, Lawrence C, Verpoorte R. Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv 2005; 23(4):283-333. [PubMed]

15. Xu Y, Xia X. Induction of ganoderic acid biosynthesis by Mn2+ in static liquid cultivation of Ganoderma lucidum. Biotechnol Bioeng 2014; 111(11):2358-65. [PubMed]

16. Zhu L, Zhong J, Tang Y. Significance of fungal elicitors on the production of ganoderic acid and Ganoderma polysaccharides by the submerged culture of medicinal mushroom Ganoderma lucidum. Process Biochem 2008; 43(1):1359-70. [Article]

17. Liu G, Xiao H, Wang X, Zhang G, Ren G. Stimulated Production of Triterpenoids of Ganoderma lucidum by an Ether Extract from the Medicinal Insect, Catharsius molossus, and Identification of the Key Stimulating Active Components. Appl Biochem Biotechnol 2011; 165(1):87-97. [PubMed]

18. Liang C, Li Y, Xu J, Wang J, Miao X, Tang Y. Enhanced biosynthetic gene expressions and production of ganoderic acids in static liquid culture of Ganoderma lucidum under phenobarbital induction. Appl Microbiol Biotechnol 2010; 86(5):1367-74. [PubMed]

19. Nojoki F, Hatamian AS, Mirderikvand M, Ebrahimi B, Mokhtari ZB, Kalantari S, Esmaeilifar M. Impact of Rifampin Induction on the Fermentation Production of Ganoderic Acids by Medicinal Mushroom Ganoderma lucidum. Appl Food Biotechnol 2016; 3(2):91-8. [Article]

20. Ren A, Qin L, Shi L, Dong X, Mu D, Li X. Methyl jasmonate induces ganoderic acid biosynthesis in the basidiomycetous fungus Ganoderma lucidum. Bioresour Technol 2010; 101(17): 6785-90. [PubMed]

21. You B, Lee M, Tien N, Lee M, Hsieh H. A Novel Approach to Enhancing Ganoderic Acid Production by Ganoderma lucidum Using Apoptosis Inducion. PLoS One 2013; 8(1):1-7. [PubMed]

22. Zhu W, Zhong J, Tang Y. Significance of fungal elicitors on the production of ganoderic acid and Ganoderma polysaccharides by the submerged culture of medicinal mushroom Ganoderma lucidum. Process Biochem 2008; 43(1):1359-70. [Article]

23. Xu J, Zhao W, Zhong J. Biotechnological production and application of ganoderic acids. Appl Microb Biotechnol 2010; 87(2):457-66. [PubMed]