year 4, Issue 1 And 2 (9-2010)                   Iran J Med Microbiol 2010, 4(1 And 2): 36-41 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Behrouzi A, Rahbar M, Vand Yousefi J. The prevalence of Extended-Spectrum β-lactamase (ESBLs) producing Klebsiella pneumonia and Eschrrichia coli isolated in Milad hospital of Tehran in 2010. Iran J Med Microbiol 2010; 4 (1 and 2) :36-41
URL: http://ijmm.ir/article-1-57-en.html
1- Deprtment of micobiology, Islamic Azad university, Karadj branche, Iran
2- Deprtment of microbiology, Reference laboratory , Tehran, Iran , rahbar_reflab@yahoo.com
Abstract:   (23719 Views)
Background and Objectives: Extended – Spectrum beta – lactamase (ESBLs) are certain enzymes that are produced by Gram- negative bacilli such as K. pneumoniae and E .coli, which cause resistance of these organisms to penicillins, cephalosporins, and monobactams. The aim of this study was to determine prevalence of Extended-Spectrum β-lactamase (ESBL) producing E. coli and K. pneumoniea isolated from urine cultures in Milad hospital of Tehran in 2010.
Material and Methods: This study was performed on 735 Gram negative bacilli isolated from urine of patients admitted to Milad hospital of Tehran from March to June 2009 and which included 620 (84.3%) strains of E. coli and 115 (15.7%) strains of K. pneumoniea isolates. From antibacterial susceptibility testing as well as for beta-lactamase production disk diffusion method and combined disk diffusion technique were employed respectively, as recommended clinical laboratory standards institutes (CLSI).
Results: ESBLs resistance was detected in 132 (21%) of the E.coli isolates and 18 (12%) of the K. pneumoniae strains. Of the 150 patients which had positive ESBLs isolates, 104 of them were outpatients and the other 46 were hospitalized. Resistance of ESBLs producing isolates of K. pneumoniae to carbenicillin, ampicillin and amikacin was 99.95%, 100% and 78 %, respectively. The least percentage of resistance among ESBLs positive isolates were observed against ofloxacin (28%). We observed high rate of resistance among isolates of E.coli to ampicillin (85%), tetracycline (62%), and trimethoprim /sulfamethoxazole (54%). However, the least rate of resistance was observed among 3 (0.5%) ESBLs positive isolates of E. coli against nitrofurantoin.
Conclusion: About one fifth of the E. coli and roughly one sixth of the K. pneumoniae isolates were producers of ESBLs. Resistance to other commonly used antibiotics among ESBLs producing isolateswere prevalent.
Full-Text [PDF 222 kb]   (3663 Downloads)    
Type of Study: Original Research Article | Subject: Nosocomial infections
Received: 2013/11/10 | Accepted: 2013/11/10 | ePublished: 2013/11/10

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc