Study of class 1 and 2 integrons and antimicrobial resistance in Salmonella isolated from broiler chicks in Chaharmahal va Bakhtiari province

Doosti Irani, Mehrnoosh; Faghani, Mostafa; Zia jahromi, Noosha

year 10, Issue 5 (November - December 2016) Iran J Med Microbiol 2016, 10(5): 38-44 | Back to browse issues page

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Doosti Irani M, Faghani M, Zia jahromi N. Study of class 1 and 2 integrons and antimicrobial resistance in Salmonella isolated from broiler chicks in Chaharmahal va Bakhtiari province. Iran J Med Microbiol 2016; 10 (5) :38-44
URL: http://ijmm.ir/article-1-540-en.html

Study of class 1 and 2 integrons and antimicrobial resistance in Salmonella isolated from broiler chicks in Chaharmahal va Bakhtiari province

Mehrnoosh Doosti Irani¹

, Mostafa Faghani

², Noosha Zia jahromi¹

1- Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2- Department of Animal Sciences, Faculty of Agriculture, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran , mostafafaghani@yahoo.com

Abstract: (8033 Views)

Background and Aim: High prevalent of drug resistant in Salmonella is a threat to human’s health. Integrons are one of the most important factors that can contribute to the occurrence of MDR bacteria. The aim of this study was to determine the prevalence of class 1, 2 integrons among Salmonella strains isolated from broiler chicks.

Materials and Methods: This study was performed on 100 Salmonella i solated strains, collected from broiler chicks samples during the summer of 2015 in Chaharmahal va Bakhtiari. The prevalence of class 1and 2 integrons were verified using specific primers by multiplex PCR assay. Susceptibility to antimicrobial agents was tested using the disk diffusion method according to the CLSI 2011. In the end integrons prevalence and drug resistance were analyzed by using SPSS software.

Results: Screening of Salmonella isolates revealed the prevalence of class 1, 2 integrons (50%), (28%) and (48%), respectively. Based on the results of the antibiogram test, the highest rate of antibiotic resistance was Ampicillin among all isolates. The highest rate of antibiotic resistance in samples of IntI 1-positive have been to Gentamycin and Tetracycline (16%) and in IntI 2-positive have been to Gentamycin (35.7%). Also the intermediate resistant strains to Chloramphenicol in the samples of IntI 1-positive were 72% and in the samples of IntI 2-positive were 42.9%.

Conclusions: The presence of two classes of integrons and its direct connection with the MDR in Salmonella is concerned. Based on the results of this study, significant correlations were between MDR and integrons that are a serious problem in human and veterinary medicine.

Keywords: Salmonella, Integrons, Drug resistance, Broiler chicks

Full-Text [PDF 661 kb] (2246 Downloads)

Type of Study: Original Research Article | Subject: Food Microbiology
Received: 2016/04/5 | Accepted: 2016/09/17 | ePublished: 2016/10/17

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