year 10, Issue 3 (July - August 2016)                   Iran J Med Microbiol 2016, 10(3): 39-46 | Back to browse issues page

PMCID: 8035013

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Lack of relatedness between Human cytomegalovirus in semen and male infertility
Farzaneh Tafvizi 1, Khatereh Baghdadi2 , Nasim Hayati Roodbari3
1- Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran , farzanehtafvizi54@gmail.com
2- Department of Biology, Islamic Azad University, Arak Branch, Arak, Iran
3- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract:   (10332 Views)

Background and Aim: Infertility refer to an inability to conceive (irrespective of causes) after having regular unprotected sex. Men are responsible for infertility in 50% of infertile spouses. Numerous factors contribute to male infertility including genital infections that may appear following microbial, fungal, and viral infections. The aim of the present study was detection of Human cytomegalovirus (HCMV) infection in the semen of infertile men and evaluation of its relation with sperm parameters such as motility, count and morphology.

Materials and Methods: In this study, 100 semen samples of fertile men (as a control group) and 100 semen samples of infertile men were collected from Infertility Center of Qom Jahad Daneshgahi, Iran. The semen samples were analyzed according to World Health Organization’s standard methods (WHO). After DNA extraction, cytomegalovirus was detected using Nested polymerase chain reaction technique. The results were analyzed using SPSS (version 19) software (Significance level of P<0.05). Statistical assessments were conducted using descriptive statistics including the Chi-square test and t test. The difference was considered significant at the P<0.05 level.

Results: The analyzed sperm parameters of 100 infertile samples showed that 56%, 70%, and 82% of the samples had problems, respectively in terms of count, motility, and morphology. However, 60% of the infertile samples had problems in all three parameters. Human cytomegalovirus DNA was detected in six and four samples of infertile and fertile men respectively.

Conclusions: According to statistical analysis, no significant correlation was obtained between the cytomegalovirus infection and male infertility. Although the achieved results may vary with changes in population size.

Keywords: Male infertility, Human cytomegalovirus, sperm quality, Nested-PCR
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Type of Study: Original Research Article | Subject: Medical Virology
Received: 2016/01/1 | Accepted: 2016/04/9 | ePublished: 2016/10/16
References
1. Ahmad MK, Mahdi AA, Shukla KK, Islam N, Jaiswar SP, Ahmad S. Effect of Mucunapruriens on semen profile and biochemical parameters in seminal plasma of infertile men. Fertil Steril. 2008; 90:627-3. [PubMed]
2. La Vignera S, Condorelli R, Vicari E, D’Agata R, Calogero AE. Diabetes mellitus and sperm parameters. J Androl. 2012;33:145-53. [PubMed]
3. Revello MG, Gerna G. Diagnosis and management of human cytomegalovirus infection in the mother, fetus, and newborn infant. Clin Microbiol Rev. 2002; 15:680-715. [PubMed]
4. Garollaa A, Pizzola D, Bertoldoa A, Menegazzoa M, Barzonb L, Forestaa C. Sperm viral infection and male infertility: focus on HBV, HCV, HIV, HPV, HSV, HCMV, and AAV. J Reprod Immunol. 2013; 100(1):20-9. [PubMed]
5. World Health Organization. WHO laboratory manual for the examination and processing of human semen. Cambridge: Cambridge University Press; 2010; 1-271.
6. Weyrich A. Preparation of Genomic DNA from Mammalian Sperm. Current protocols in Molecular Biology. Berlin, Germany. 2012; 17: 1-4. [Article]
7. Kühn JE, Wendland T, Eggers HJ, Lorentzen E, Wieland U, Eing B, Kiessling M, Gass P. Quantitation of human cytomegalovirus genomes in the brain of AIDS patients. J Med Virol. 1995; 47:70-82. [PubMed]
8. Berger RE. Epididymites. In Holmes, K.K., Märdh, P.A., Sparling, P.F. and Wiesner, P.J. (eds), Sexually Transmitted Diseases. McGraw-Hill, New York, 1984, pp. 650–662
9. Gimenes F, Souza RP, Bento JC, Teixeira JJ, Maria-Engler SS, Bonini MG, Consolaro ME. Male infertility: a public health issue caused by sexually transmitted pathogens. Nat Rev Urol. 2014;11(12):672-87. doi: 10.1038/nrurol.2014.285. [PubMed]
10. Keck C, Gerber-Schafer C, Clad A, Wilhelm C, Breckwoldt M.,. Seminal tract infections: impact on male fertility and treatment options. Hum Reprod Update.1998; 4:6,891-903. [PubMed]
11. Friberg J. Mycoplasmas and ureaplasmas in infertility and abortion. Fertil Steril. 1980; 33(4):351-9. [PubMed]
12. Colpi GM, Negri L. Inflammatory pathology of the genital tract and male infertility.Acta Eur Fertil 1989; 20:6,125-127. [PubMed]
13. Eggert-Kruse W, Reuland M, Johannsen W, Strowitzki T. Schlehofer, JR. Cytomegalovirus (CMV) infection—related to male and/or female infertility factors? Fertil Steril. 2009; 91: 67–82. [PubMed]
14. Neofytou E, Sourvinos G, Asmarianaki M, Spandidos DA, Makrigian-nakis A. Prevalence of human herpes virus types 1–7 in these men of men attending an infertility clinic and correlation with semen parameters. Fertil Steril. 2009; 91: 2487–2494. [PubMed]
15. Bresson JL, Clavequin MC, Mazeron MC, Mengelle C, Scieux C, Segondy M, Houhou N; Fédération Française des CECOS. Risk of cytomegalovirus transmission by cryopreserved semen: a study of 635 semen samples from 231 donors. Hum Reprod. 2003; 18: 1881–1886. [PubMed]
16. Kapranos N, Petrakou E, Anastasiadou C, Kotronia. Detection of herpes simplex virus, cytomegalovirus, and Epstein-Barr virus in the semen of men attending an infertility clinic. Fertil Steril. 2003; 79:1566-1570. [PubMed]
17. McGowan MP, Hayes K, Kovacs GT, Leydon JA. Prevalence of cytomegalovirus and herpes simplex virus in human semen. Int J Androl 1983; 6:331–336. [PubMed]
18. Yang YS, Ho HN, Chen HF, Chen SU, Shen CY, Chang SF, Huang ES, Wu CW. Cytomegalovirus infection and viral shedding in the genital tract of infertile couples. J Med Virol. 1995; 45:179–82. [PubMed]
19. Pallier C, Tebourbi L, Chopineau-Proust S, Schoevaert D, Nordmann P, Testart J, Courtot AM. Herpesvirus, cytomegalovirus, human sperm and assisted fertilization. Hum Reprod. 2002; 17: 1281–1287. [Article]
20. Habibi M, Bahrami A, Morteza A, Sadighi Gilani MA, Hassanzadeh G, Ghadami M, Choobineh H. Study of cytomegalovirus infection in idiopathic infertility men referred to Shariati hospital, Tehran, Iran. Iran J Reprod Med. 2014; 12: 151-154. [PubMed]
21. Wu KH, Zhou QK, Huang JH, Lai RQ, Lin FH, Li B, Zhang CB, Zhou WN, Zhu ZP. Infection of cytomegalovirus and herpes simplex virus and morphology of the infected spermatogenic cells in infertile men. Zhonghua Nan Ke Xue. 2007; 13:1075-1079. [PubMed]
22. Bezold G, Politch JA, Kiviat NB, Kuypers JM, Wolff H, Aderson DJ. Prevalence of sexually transmissible pathogens in semen from asymptomatic male infertility patients with and without leukocytospermia. Fertile Steril. 2007; 87:1087-1097. [PubMed]
23. Fowler KB, Pass RF. Risk factors for congenital cytomegalovirus infection in the offspring of young women: exposure to young children and recent onset of sexual activity. Pediatrics. 2006;118:e286-92. [PubMed]
24. Francisse S, Revelard P, De Maertelaer V, Strebelle E, Englert Y, Liesnard C. Human cytomegalovirus seroprevalence and risk of seroconversion in a fertility clinic population. Obstet Gynecol. 2009;114 (2 Pt 1):285-91. doi: 10.1097/AOG.0b013e3181af3d6f. [PubMed]
25. Adler SP. Screening for cytomegalovirus during pregnancy. Infect Dis Obstet Gynecol. 2011;2011:1–9 [Article]
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