year 10, Issue 4 (September - October 2016)                   Iran J Med Microbiol 2016, 10(4): 17-24 | Back to browse issues page

XML Persian Abstract Print


1- Department of Microbiology, faculty of science, Islamic Azad University, Save Branch, Save, Iran
2- Molecular Biology Research Center, Baqiyatallah University of Medical Science, Tehran, Iran , ranjbarre@gmail.com
3- Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran-Iran
Abstract:   (10636 Views)

Background and Aim: Escherichia coli  is considered  as  one  of  the  most  important  causes  of   bacterial    infections   transmitted   through   water   and  foods. Several factors are involved in the pathogenesis of Escherichia coli  .  The aim  of  this  study  was  to   investigate  the  prevalence of Escherichia coli  strains carrying  virulence gene  intimin isolated from different water sources in  Alborz province.

Materials and Methods: This study was carried  out in 2013.   The  research  included   all  E. coli  strains isolated from different surface water sources in Alborz province. Escherichia coli isolates were detected and identified by standard microbiological and biochemical tests. The strains were evaluated for the presence of intimin gene by  PCR using specific primers.

Results: One hundred E. coli  strains  were isolated  then PCR was successfully optimized for detection of intimin gene. The  PCR  results  showed  33%  of  the  strains carried out  intimin  gene.

Conclusions: Compared to many studies, our findings showed the high frequency of E. coli  strains carrying  intimin gene  isolated from surface water sources. The presence of this virulence genes is a potential threat for contamination of drinking water which is important for health care systems.

Full-Text [PDF 741 kb]   (4817 Downloads)    
Type of Study: Original Research Article | Subject: Molecular Microbiology
Received: 2015/04/8 | Accepted: 2015/05/25 | ePublished: 2016/10/16

References
1. Bonyadian M, Salehi T, Mahzounieh M, Taheri F. Virulence genes of verotoxigenic E. coli isolated from raw milk and unpasturized cheese. J Vet Res 2011;66(3):223-8, 81. [Article]
2. Kargar M, Dianati P, Homayoon M. Evolution of Virulence Genes and Antibiotic Resistance of Enterohemorrhagic Escherichia coli Isolated from Hamburger by Multiplex PCR in Shiraz. J Isfahan Med Sch 2011;29(148). [Article]
3. Munshi SK, Rahman MM, Noor R. Detection of Virulence Potential of Diarrhoeagenic Escherichia coli Isolated from Surface Water of Rivers Surrounding Dhaka City. J Bangladesh Acad Sci 2012;36(1):109-21. [Article]
4. Rompré A, Servais P, Baudart J, de-Roubin M-R, Laurent P. Detection and enumeration of coliforms in drinking water: current methods and emerging approaches. J microbiol methods 2002;49(1):31-54. [PubMed]
5. Edberg S, Rice E, Karlin R, Allen M. Escherichia coli: the best biological drinking water indicator for public health protection. J Appl Microbiol 2000;88(S1)106-16 [PubMed]
6. Odonkor ST, Ampofo JK. Escherichia coli as an indicator of bacteriological quality of water: an overview. Microbiology research 2013;4(1):2. [Article]
7. Karouei H, Masoud S, Eslamifar M, Zazouli MA. Determination of fecal coliform contamination of water supplies in some rural areas of Sari, Iran with most probable number test. J Mazandaran Univ Med Sci 2013;23(104):89-95. [Article]
8. Zazouli MA, Safarpour Ghadi M, Veisi A, Habibkhani P. Bacterial contamination in bottled water and drinking water distribution network in semnan, 2012. J Mazandaran Univ Med Sci 2013;22(1):151-9. [Article]
9. Bonacorsi S, Bingen E. Molecular epidemiology of Escherichia coli causing neonatal meningitis. Int J Med Microbiol 2005 ; 295 (6): 373-81.
10. Jeong H, Han S-j, Yoo H-N, Choi S-J, Oh S-Y, Kim Y-J, et al. Comparison of changes in etiologic microorganisms causing early-onset neonatal sepsis between preterm labor and preterm premature rupture of membranes. J Matern-Fetal Neonatal Med 2015;28(16):1923-8. [PubMed]
11. Shah BA, Padbury JF. Neonatal sepsis: an old problem with new insights. Virulence. 2014;5(1):170-8. [PubMed]
12. Molaabaszadeh H, Hajisheikhzadeh B, Mollazadeh M, Eslami K, Mohammadzadeh Gheshlaghi N. Study of sensibility and antimicrobial resistance in Escherichia coli isolated from urinary tract infection in Tabriz city. J Fasa Univ Med Sci 2013;3(2):149-54. [Article]
13. Rajendran P, Ajjampur SSR, Chidambaram D, Chandrabose G, Thangaraj B, Sarkar R, et al. Pathotypes of diarrheagenic Escherichia coli in children attending a tertiary care hospital in South India. Diagn microbiol infect dis 2010;68(2):117-22.
14. Safarpordehkordi F, Yahaghi E, Khodaverdi Darian E. Prevalence of Antibiotic Resistance in Escherichia coli Isolated from Poultry Meat Supply in Isfahan. Iran J Med Microbiol 2014;8(2):41-7. [Article]
15. Nataro JP, Kaper JB. Diarrheagenic Escherichia coli. Clin Microbiol Rev 1998;11(1):142 -201 [PubMed]
16. Kosek M, Bern C, Guerrant RL. The global burden of diarrhoeal disease, as estimated from studies published between 1992 and 2000. Bull World Health Organ 2003;81(3):197-204. [PubMed]
17. Gomez-Duarte OG, Romero-Herazo YC, Paez-Canro CZ, Eslava-Schmalbach JH, Arzuza O. Enterotoxigenic Escherichia coli associated with childhood diarrhoea in Colombia, South America. J Infect Dev Ctries 2013;7(05):372-81. [PubMed]
18. Guerrant RL, Hughes JM, Lima NL, Crane J. Diarrhea in developed and developing countries: magnitude, special settings, and etiologies. Rev infect dis 1990;12(Supplement 1):S41-S50. [PubMed]
19. Okeke IN, Lamikanra A, Steinrück H, Kaper JB. Characterization of Escherichia colistrains from cases of childhood diarrhea in provincial southwestern Nigeria. J clin microbiol 2000;38(1):7-12. [PubMed]
20. Abbasi M, Aslani MM, Mostafavi E, Alikhani MY, Nikbin VS. Determination of Adhesion Virulence Factors of Enteropathogenic Escherichia coli (eaeA-, bfpA-) Isolates from Asymptomatic Individuals Compared to those with Diarrhea. Modares J Med Sci Pathol 2013;15(4):99-108. [Article]
21. Kargar M, Daneshvar M, Homayoun M. Prevalence of shiga toxins, intimin and hemolysin genes of Escherichia coli O157: H7 strains from industrial ground meat in Shiraz. J Shaheed Sadoughi Univ Med Sci 2011;18(675): 512-520
22. Saberi F, Salmanian AH, Amani J, Jafari M. Production of Chimeric Tir-intimin Protein from E. coli O157: H7 in the Tobacco (Nicotiana tobbacum) Plant and its Immunological Evaluation in an Animal Model. Modares J Med Sci Pathol 2012;15(3):23-36. [Article]
23. Louie M, De Azavedo J, Handelsman M, Clark C, Ally B, Dytoc M, et al. Expression and characterization of the eaeA gene product of Escherichia coli serotype O157: H7. Infect immun 1993;61(10):4085-92. [PubMed]
24. Adeli Z, Firoozeh F, Zibaei M, Shakib P. Prevalence of Shiga toxin and Intimine genes in Shiga toxin-producing Escherichia coli isolated from urine samples in Lorestan, Iran. Feyz J Kashan Univ Med Sci 2013;17(2). [Article]
25. Association APH, Association AWW, Federation WPC, Federation WE. Standard methods for the examination of water and wastewater: APHA.; 1915.
26. Akter S, Islam M, Afreen K, Azmuda N, Khan S, Birkeland N. Prevalence and distribution of different diarrhoeagenic Escherichia coli virulotypes in major water bodies in Bangladesh. Epidemiol infect 2013;141(12):2516-25. [PubMed]
27. Dallal MMS, Sepehri S, Bafrouei AT, Khiabani ZD. Determination of genotype variation of Escherichia coli in well water of Tehran’s parks by Multiplex PCR. Pajoohandeh Journal. 2011;16(5):226-33. [Article]
28. Widmer K, Van Ha NT, Vinitnantharat S, Sthiannopkao S, Wangsaatmaja S, Prasetiati MAN, et al. Prevalence of Escherichia coli in surface waters of Southeast Asian cities. World J Microbiol Biotechnol 2013;29(11):2115-24. [PubMed]
29. Halabi M, Orth D, Grif K, Wiesholzer-Pittl M, Kainz M, Schöberl J, et al. Prevalence of Shiga toxin-, intimin-and haemolysin genes in Escherichia coli isolates from drinking water supplies in a rural area of Austria. Int j hyg environ health 2008;211(3):454-7. [PubMed]
30. Sidhu JP, Ahmed W, Hodgers L, Toze S. Occurrence of virulence genes associated with diarrheagenic pathotypes in Escherichia coli isolates from surface water. Appl environ microbiol 2013;79(1):328-35. [PubMed]

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.