year 19, Issue 5 (September - October 2025)
Iran J Med Microbiol 2025, 19(5): 310-311 |
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Hajisadeghi S, Pourrabbani Z, Fateh R, Salesi M, Keykha E. Impact of Turpentine-Containing Chewing Gum on Saliva Characteristics and Streptococcus mutans in Young Adults. Iran J Med Microbiol 2025; 19 (5) :310-311
URL: http://ijmm.ir/article-1-2790-en.html
URL: http://ijmm.ir/article-1-2790-en.html
1- Research Center for Prevention of Oral and Dental Diseases, Dental School, Baqiyatallah University of Medical Sciences, Tehran, Iran
2- Faculty of Dentistry, Qom University of Medical Sciences, Qom, Iran
3- Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran & Department of Microbiology and Immunology, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran
4- Research Center for Prevention of Oral and Dental Diseases, Dental School, Baqiyatallah University of Medical Sciences, Tehran, Iran ,dr.keykha@chmail.ir
2- Faculty of Dentistry, Qom University of Medical Sciences, Qom, Iran
3- Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran & Department of Microbiology and Immunology, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran
4- Research Center for Prevention of Oral and Dental Diseases, Dental School, Baqiyatallah University of Medical Sciences, Tehran, Iran ,
Abstract: (294 Views)
Background: Tooth decay is one of the most common oral diseases. The pH and amount of saliva affect the growth rate of caries-causing bacteria. Chewing gum can help reduce caries by affecting saliva. The aim of this study was to compare the effect of several types of chewing gums available in the Iranian market on pH, volume, buffering capacity of saliva and the number of salivary Streptococcus mutans.
Methods: Forty dentistry students were divided into four groups. Each group was given a brand of gum (Van-Xylitol/Van-Glucose/Chic/Triadent). In this study, participants were asked to chew gum for 20 minutes after each meal. Non-stimulated saliva was collected by spitting method at the beginning of the study and three weeks after gum consumption. pH, volume, and salivary buffering capacity were determined. In addition, the amount of S.mutans in the saliva was evaluated.
Results: The amount of salivary S. mutans in all groups of consumers dropped (P<0.05) and saliva volume increased after three weeks of gum chewing. Saliva pH, saliva volume, salivary buffering capacity, and the number of salivary streptococci all significantly changed after chewing van xylitol gum.
Conclusion: Van-Xylitol and Triedent gums were most effective in improving salivary volume, and reducing the number of S. mutans in saliva, but Van-Xylitol was the most effective among the groups in improving salivary pH and increase the buffering capacity of saliva. It seems that turpentine can significantly enhance the properties of xylitol-containing gums.
Methods: Forty dentistry students were divided into four groups. Each group was given a brand of gum (Van-Xylitol/Van-Glucose/Chic/Triadent). In this study, participants were asked to chew gum for 20 minutes after each meal. Non-stimulated saliva was collected by spitting method at the beginning of the study and three weeks after gum consumption. pH, volume, and salivary buffering capacity were determined. In addition, the amount of S.mutans in the saliva was evaluated.
Results: The amount of salivary S. mutans in all groups of consumers dropped (P<0.05) and saliva volume increased after three weeks of gum chewing. Saliva pH, saliva volume, salivary buffering capacity, and the number of salivary streptococci all significantly changed after chewing van xylitol gum.
Conclusion: Van-Xylitol and Triedent gums were most effective in improving salivary volume, and reducing the number of S. mutans in saliva, but Van-Xylitol was the most effective among the groups in improving salivary pH and increase the buffering capacity of saliva. It seems that turpentine can significantly enhance the properties of xylitol-containing gums.
Type of Study: Original Research Article |
Subject:
Oral Microbiology
Received: 2025/07/22 | Accepted: 2025/10/27 | ePublished: 2025/11/11
Received: 2025/07/22 | Accepted: 2025/10/27 | ePublished: 2025/11/11
References
1. Signori C, Laske M, Bronkhorst EM, Huysmans MCD, Cenci MS, Opdam NJ. Impact of individual-risk factors on caries treatment performed by general dental practitioners. J Dent. 2019;81:85-90. [DOI:10.1016/j.jdent.2018.12.016] [PMID]
2. Pitts NB, Wright JP. Reminova and EAER: keeping enamel whole through caries remineralization. Adv Dent Res. 2018;29(1):48-54. [DOI:10.1177/0022034517737026] [PMID]
3. Gao X, Jiang S, Koh D, Hsu CYS. Salivary biomarkers for dental caries. Periodontol 2000. 2016;70(1):128-41. [DOI:10.1111/prd.12100] [PMID]
4. Wei SH, Crall JJ, Wefel JS. Dental caries: resistance factors-enamel chemistry and saliva. Systemized Prevent Oral Dis. 2019:43-59. [DOI:10.1201/9780429283758-4]
5. Padminee K, Poorni S, Diana D, Duraivel D, Srinivasan MR. Effectiveness of casein phosphopeptide-amorphous calcium phosphate and xylitol chewing gums on salivary pH, buffer capacity, and Streptococcus mutans levels: An interventional study. Indian J Dent Res. 2018;29(5):616-21. [DOI:10.4103/ijdr.IJDR_166_17] [PMID]
6. Bakhshi M, Kavei D, Asayesh H, Mehdipour A. Evaluating the effect of pregnancy on streptococcus mutans, ph and buffering capacity of saliva. Stud Med Sci. 2015;26(6):467-74.
7. Afshar H, Nahvi A. Investigating the sweeteners of current gums present in Iranian market. Iran J Pediatr Dent. 2014;9(2):19-24. [DOI:10.29252/ijpd.9.2.19]
8. Mazhari F, Izadi EF. Comparison of the Effect of Natural Turpentine and Synthetic Sugar Free Gums on Dental Plaque pH Recovery. J Dent Mater Tech. 2018;7(3):111-6.
9. de Siqueira Fraga EG, Neto EMR, Campos FMT, da Silva Cavalcante MP, Amorim FA, Martins LFB, et al. Evaluation of chewing gums containing natural products on salivary concentrations of streptococcus mutans in Children. J Young Pharm. 2022;15(1):182-8. [DOI:10.5530/097515050561]
10. Ahmadi-Motamayel F, Akbari E, Mahjoub R, Alikhani MY, Poorolajal J. Effect of chewing gum containing glycyrrhiza glabra, honey, and vitamin E on oral health. J Herb Med 2024;43:100831. [DOI:10.1016/j.hermed.2023.100831]
11. Weber J, Scholz KJ, Schenke IM, Pfab F, Cieplik F, Hiller KA, et al. Randomized controlled clinical trial on the efficacy of a novel antimicrobial chewing gum in reducing plaque and gingivitis in adolescent orthodontic patients. Clin Oral Investig. 2024;28(5):272. [DOI:10.1007/s00784-024-05669-4] [PMID] [PMCID]
12. Daryaei M, Hoseiny S, Taheri K, Mirzaei J, Mzbani A. Effect of morphological variables of Pistacia atlantica on gum and seed production. Iran J Biol. 2012;25(2):303-15.
13. Niculescu A-G, Grumezescu AM. Natural compounds for preventing ear, nose, and throat-related oral infections. Plants. 2021;10(9):1847. [DOI:10.3390/plants10091847] [PMID] [PMCID]
14. Esentürk-Güzel İ, Abdo L, Topuzoğlu S, YILDIZ C, YILMAZ F, DÖŞLER S. Natural ingredients included antimicrobial lozenge formulations for oral care. J Res Pharm. 2024;28(1):248-57. [DOI:10.29228/jrp.692]
15. Mobaraki N, Amani F, Najafi E, Gamisi J. Comparison of the Effect of Coffee and Chewing Gum on the Return of Gastrointestinal Motility in Patients Undergoing Cesarean Section. Complement Med Res. 2024;14(3):21-6.
16. Tahmourespour A, Aminzadeh A, Salehifard I. Anti-adherence and anti-bacterial activities of Pistacia atlantica resin extract against strongly adherent Streptococcus mutans strains. Dent Res J. 2022;19(1):36. [DOI:10.4103/1735-3327.344159] [PMID]
17. Ly KA, Milgrom P, Rothen M. The potential of dental-protective chewing gum in oral health interventions. J Am Dent Assoc. 2008;139(5):553-63. [DOI:10.14219/jada.archive.2008.0215] [PMID]
18. Madrid-Troconis CC, Perez-Puello SdC. Casein phosphopeptide-amorphous calcium phosphate nanocomplex (CPP-ACP) in dentistry: State of the art. Rev Fac Odontol Univ Antioq. 2019;30(2):248-62. [DOI:10.17533/udea.rfo.v30n2a10]
19. Agarwal B, Singh LK. Sugar and Sugar Alcohols: Xylitol. High Value Fermentation Products: Human Health. 2019;1:285-307. [DOI:10.1002/9781119460053.ch12]
20. Kumar K, Singh E, Shrivastava S. Microbial xylitol production. Appl Microbiol Biotechnol. 2022;106(3):971-9. [DOI:10.1007/s00253-022-11793-6] [PMID]
21. Tusi SK, Jafari A, Marashi SM, Niknam SF, Farid M. Effect of teucrium polium-containing chewing gum on reducing salivary streptococcus mutans counts. J Mashhad Dent Sch. 2018;42(2):141-50.
22. Rafeek R, Carrington CV, Gomez A, Harkins D, Torralba M, Kuelbs C, et al. Xylitol and sorbitol effects on the microbiome of saliva and plaque. J Oral Microbiol. 2019;11(1):1536181. [DOI:10.1080/20002297.2018.1536181] [PMID] [PMCID]
23. Navazesh M. Methods for collecting saliva. Ann N Y Acad Sci. 1993;694(1):72-7. [DOI:10.1111/j.1749-6632.1993.tb18343.x] [PMID]
24. Mahjoub S, Ghasempour M, Gharage A, Bijani A, Masrourroudsari J. Comparison of total antioxidant capacity in saliva of children with severe early childhood caries and caries-free children. Caries Res. 2014;48(4):271-5. [DOI:10.1159/000355581] [PMID]
25. Yaghini J, Shirani F, Mogharehabed A, Karimipour H, Sarsangi M. Evaluation of DMFT, salivary pH and salivary buffering capacity in patients with and without calculus referring to Isfahan Faculty of Dentistry. J Isfahan Dent Sch. 2011;6(5):820-7.
26. Kamate WI, Vibhute NA, Baad RK. Estimation of DMFT, salivary streptococcus mutans count, flow rate, pH, and salivary total calcium content in pregnant and non-pregnant women: a prospective study. J Clin Diagn Res. 2017;11(4):ZC147. [DOI:10.7860/JCDR/2017/24965.9516] [PMID] [PMCID]
27. Higuchi M. The effect of oxygen on the growth and mannitol fermentation of Streptococcus mutans. Microbiology. 1984;130(7):1819-26. [DOI:10.1099/00221287-130-7-1819] [PMID]
28. Ma J, Hunjan M, Smith R, Kelly C, Lehner T. An investigation into the mechanism of protection by local passive immunization with monoclonal antibodies against Streptococcus mutans. Infect Immun. 1990;58(10):3407-14. [DOI:10.1128/iai.58.10.3407-3414.1990] [PMID] [PMCID]
29. Karpiński TM, Szkaradkiewicz AK. Microbiology of dental caries. J Biol Earth Sci. 2013;3(1):M21-4.
30. Vos T, Flaxman AD, Naghavi M, Lozano R, Michaud C, Ezzati M, et al. Years lived with disability (YLDs) for 1160 sequelae of 289 diseases and injuries 1990-2010: a systematic analysis for the Global Burden of Disease Study 2010. Lancet. 2012;380(9859):2163-96. [DOI:10.1016/S0140-6736(12)61729-2] [PMID]
31. Mohammadzadeh M, Babaeifar F, Babaei F. Combination thrapy of hydroalcoholic extract of Pistacia atlantica kurdica and fluvoxamine on spatial memory of immobilization rat. J Gorgan Univ Med Sci. 2017;19(3):24-31.
32. Khamverdi Z, Farhadian F, Khazaei S, Adabi M. Efficacy of chitosan-based chewing gum on reducing salivary S. mutans counts and salivary pH: a randomised clinical trial. Acta Odontol Scand. 2021;79(4):268-74. [DOI:10.1080/00016357.2020.1836392] [PMID]
33. Shinde MR, Winnier J. Comparative evaluation of Stevia and Xylitol chewing gum on salivary Streptococcus mutans count-A pilot study. J Clin Exp Dent. 2020;12(6):e568. [DOI:10.4317/jced.55720] [PMID] [PMCID]
34. Prathima GS, Narmatha M, Selvabalaji A, Adimoulame S, Ezhumalai G. Effects of Xylitol and CPP-ACP Chewing Gum on Salivary Properties of Children with Molar Incisor Hypomineralization. Int J Clin Pediatr Dent. 2021;14(3):412. [DOI:10.5005/jp-journals-10005-1779] [PMID] [PMCID]
35. Shinde MR, Winnier J. Effects of stevia and xylitol chewing gums on salivary flow rate, pH, and taste acceptance. J Dent Res Rev. 2020;7(2):50-5.
36. Nazri WA, Daud W, Rumiati F. Characteristics of saliva in FK Ukrida students after chewing paraffin, xylitol and sucrose gum. Indones J Biotechnol Biodivers. 2021;5(1):24-30. [DOI:10.47007/ijobb.v5i1.73]
37. Dodds MW, Haddou MB, Day JE. The effect of gum chewing on xerostomia and salivary flow rate in elderly and medically compromised subjects: a systematic review and meta-analysis. BMC Oral Health. 2023;23(1):406. [DOI:10.1186/s12903-023-03084-x] [PMID] [PMCID]
38. Hegde RJ, Thakkar JB. Comparative evaluation of the effects of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and xylitol-containing chewing gum on salivary flow rate, pH and buffering capacity in children: An in vivo study. J Indian Soc Pedod Prev Dent. 2017;35(4):332-7. [DOI:10.4103/JISPPD.JISPPD_147_17] [PMID]
39. Giacaman RA, Maturana CA, Molina J, Volgenant C, Fernández CE. Effect of casein phosphopeptide-amorphous calcium phosphate added to milk, chewing gum, and candy on dental caries: a systematic review. Caries Res. 2023;57(2):106-18. [DOI:10.1159/000530638] [PMID]
40. Akgül Ö, Topaloğlu Ak A, Zorlu S, Öner Özdaş D, Uslu M, Çayirgan D. Effects of short‐term xylitol chewing gum on pro‐inflammatory cytokines and Streptococcus mutans: A randomised, placebo‐controlled trial. Int J Clin Pract. 2020;74(9):e13623. [DOI:10.1111/ijcp.13623] [PMID]
41. Cocco F, Cagetti MG, Majdub O, Campus G. Concentration in Saliva and Antibacterial Effect of Xylitol Chewing Gum: In Vivo and In Vitro Study. Appl Sci. 2020;10(8):2900. [DOI:10.3390/app10082900]
42. Söderling E, Pienihäkkinen K. Specific effects of xylitol chewing gum on mutans streptococci levels, plaque accumulation and caries occurrence: a systematic review. BMC Oral Health. 2025;25(1):1275. [DOI:10.1186/s12903-025-06602-1] [PMID] [PMCID]
43. Arora N, Hande A. The Effect of Chewing Gum Containing Casein Phosphopeptide-Amorphous Calcium Phosphate on Salivary Streptococcus mutans. J Datta Meghe Inst Med Sci Univ. 2019;14(3):171-4. [DOI:10.4103/jdmimsu.jdmimsu_47_19]
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