year 7, Issue 3 (Fall 2013)
Iran J Med Microbiol 2013, 7(3): 12-17 |
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Rahman Shokri1 
, Bahman Tabaraee , Sedighe Hatami , Omid Nilchi zadeh , Lida Shafie abkenari , Bahram Fekri
, Bahman Tabaraee , Sedighe Hatami , Omid Nilchi zadeh , Lida Shafie abkenari , Bahram Fekri
1- , Rshokri_54@yahoo.com
Abstract: (12203 Views)
Background and Aim: Salmonella contamination is a major problem in poultry and food industry. Lack of on-time diagnosis of the bacteria may cause irreparable economic loss. Speed, repeatability and cost-benefits are the most important parameters. Faster, easier and more economic monitoring of pathogens (e.g. Salmonella.) is essential in the whole process. Routine bacterial culture methods and Antibody-Enzyme Immunoassay (ELISA) are time consuming and costly. The aim of this study was to compare Polymixin Coated Polyester Cloth (P-CEIA) with culture and ELISA in identification and rapid diagnosis of different Salmonella serovars in dairy products.
Materials and Methods: In order to compare Ab-EIA and P-CEIA, different dilutions of S. typhimorium Ra-30 were prepared in peptone water (BPW, pH=7.4). From the 20 samples of milk and cream, 10 suspicious slamonella contaminated samples had been surveyed by the two methods.
Results: The sensitivity of P-CEIA method was 106 cfu/ml while the sensitivity of ab-EIA was estimated to be 105 cfu/ml. The sensitivities of the two methods were equal following heat treatment in the presence of sodium deoxy-cholate. Ab-EIA detected 5 and 3 suspicious samples of milk and cream, respectively. PCEIA detected 3 and 6 of the contaminated samples. The first method required 14 hours less time than the other method.
Conclusion: Our data shows that P-CEIA is a rapid, economic and sustainable method for Salmonella detection in dairy products.
Materials and Methods: In order to compare Ab-EIA and P-CEIA, different dilutions of S. typhimorium Ra-30 were prepared in peptone water (BPW, pH=7.4). From the 20 samples of milk and cream, 10 suspicious slamonella contaminated samples had been surveyed by the two methods.
Results: The sensitivity of P-CEIA method was 106 cfu/ml while the sensitivity of ab-EIA was estimated to be 105 cfu/ml. The sensitivities of the two methods were equal following heat treatment in the presence of sodium deoxy-cholate. Ab-EIA detected 5 and 3 suspicious samples of milk and cream, respectively. PCEIA detected 3 and 6 of the contaminated samples. The first method required 14 hours less time than the other method.
Conclusion: Our data shows that P-CEIA is a rapid, economic and sustainable method for Salmonella detection in dairy products.
Type of Study: Original Research Article |
Subject:
Medical Bacteriology
Received: 2014/04/9 | Accepted: 2014/04/9 | ePublished: 2014/04/9
Received: 2014/04/9 | Accepted: 2014/04/9 | ePublished: 2014/04/9
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