year 5, Issue 1 And 2 (6-2011)
Iran J Med Microbiol 2011, 5(1 And 2): 28-33 |
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1- , Maryam.Qajar@yahoo.com
Abstract: (13400 Views)
Background and objectives: Producing and recognition Vitamin B12 transmitter protein from
Ecoli O157.H7
Material and Methods: btuB gene of Ecoli O157.H7 by using PCR proliferation and in pET28 a (t) vector cloned. resulted structure confirmed by means of limiting Anzyme Analysis and DNA sequence determination. pET28a(+) – btuB transformed in Ecoli Bl21 and recombinant btuB experission with His-Taq C-terminal performed successfully.
Results: a 66 kiloDoulton Band observed in gell which indicated BtuB protein.
Conclusion: Proliferation btuB gene and producing of BtuB protein with molecular weight 66 kilo Dalton
Material and Methods: btuB gene of Ecoli O157.H7 by using PCR proliferation and in pET28 a (t) vector cloned. resulted structure confirmed by means of limiting Anzyme Analysis and DNA sequence determination. pET28a(+) – btuB transformed in Ecoli Bl21 and recombinant btuB experission with His-Taq C-terminal performed successfully.
Results: a 66 kiloDoulton Band observed in gell which indicated BtuB protein.
Conclusion: Proliferation btuB gene and producing of BtuB protein with molecular weight 66 kilo Dalton
Type of Study: Original Research Article |
Subject:
Microbial Biotechnology
Received: 2013/11/30 | Accepted: 2013/11/30 | ePublished: 2013/11/30
Received: 2013/11/30 | Accepted: 2013/11/30 | ePublished: 2013/11/30
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