year 3, Issue 4 (Winter 2010)
Iran J Med Microbiol 2010, 3(4): 46-52 |
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Ghasemi Dehkordi P, Momtaz H, Rezaeian A, Yaghobi R. Molecular study of frequency of Herpes simplex virus type 2in Esfahan and Chaharmahal-va-Bakhtiari provinces in year 1388. Iran J Med Microbiol 2010; 3 (4) :46-52
URL: http://ijmm.ir/article-1-168-en.html
URL: http://ijmm.ir/article-1-168-en.html
1- Department of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom - Iran.
2- Department of Microbiology, Islamic Azad University, Shahrekord Branch, Shahrekord - Iran. , hamomtaz@iaushk.ac.ir
3- Shiraz Transplant Research Center, Namazee Hospital, University of Medical Sciences, Shiraz - Iran
2- Department of Microbiology, Islamic Azad University, Shahrekord Branch, Shahrekord - Iran. , hamomtaz@iaushk.ac.ir
3- Shiraz Transplant Research Center, Namazee Hospital, University of Medical Sciences, Shiraz - Iran
Abstract: (13439 Views)
Background and Objectives: Infections due to Herpes simlex virus type 2 (HSV-2) often causes genital
herpes in men and women, infant herpes and nonsupporative meningitis. These diseases are caused in
relation to HIV infection, and might be transfected from mother to baby.
In this study the prevalence of HSV-2was determined for the first time by PCR method in clinical samples
collected from Isfahan and Chaharmahal-va-Bakhtiari provinces.
Materials and Methods: One hundred HSV-2infected sera with high titer of IgG and IgM were collected from suspected patients. In total 82 samples from Isfahan province and 18 samples from Chaharmahal-vaBakhtiari province were collected. Viral DNA was extracted and PCR was performed by using specific primers for gD gene of HSV-2.
Results: The positive results of HSV-PCR and the 1013 bp segment of HSV-2 gDgene was detected in 8 of 100 (8%) of serum samples. In Isfahan province 6.09 % and in Chaharmahal-va-Bakhtiari province 16.66 % were positive for HSV-2.
Conclusion:The results of this study demonstrated the prevalence of HSV-2in studied region similar to other regions of the world. Besides, PCR method is presented as a useful procedure for detection of HSV-2 in infected sera.
Materials and Methods: One hundred HSV-2infected sera with high titer of IgG and IgM were collected from suspected patients. In total 82 samples from Isfahan province and 18 samples from Chaharmahal-vaBakhtiari province were collected. Viral DNA was extracted and PCR was performed by using specific primers for gD gene of HSV-2.
Results: The positive results of HSV-PCR and the 1013 bp segment of HSV-2 gDgene was detected in 8 of 100 (8%) of serum samples. In Isfahan province 6.09 % and in Chaharmahal-va-Bakhtiari province 16.66 % were positive for HSV-2.
Conclusion:The results of this study demonstrated the prevalence of HSV-2in studied region similar to other regions of the world. Besides, PCR method is presented as a useful procedure for detection of HSV-2 in infected sera.
Keywords: Herpes simplex virus type 2(HSV-2), PCR, gDgene, Chaharmahal-va-Bakhtiari province, Isfahan province
Type of Study: Original Research Article |
Subject:
Medical Virology
Received: 2013/11/22 | Accepted: 2013/11/22 | ePublished: 2013/11/22
Received: 2013/11/22 | Accepted: 2013/11/22 | ePublished: 2013/11/22
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