Khajehkarramedini M, Fazli Bazaz B S, Ebrahimi M, Ghazvini K, Afzal Aghaee M, Naderinasab M, et al . Enrichment and Isolation of Lytic Bacteriophages Against Antibiotic-Resistant Pseudomonas aeroginosa. Iran J Med Microbiol 2009; 3 (2 and 3) :66-72
URL:
http://ijmm.ir/article-1-160-en.html
1- Department of Microbiology and virology , Microbiology and virology Research Center, Mashad University of Medical Sciences, Iran
2- General practitioner
3- Department of Community Medicine, Mashad University of Medical Sciences, Iran
4- Department of Microbiology and virology , Microbiology and virology Research Center, Mashad University of Medical Sciences, Iran , meshkatz@mums.ac.ir
Abstract: (23378 Views)
Background and Objective: One of the most common problems in the hospitals is the appearance of the
resistant infections to the antimicrobial agents. Bacteriophages can use as an alternative to antibiotics for
preventing and treating bacterial infections. Bacteriophages are viruses that infect and kill bacteria. They are
safe having no activity against animal or plant cells and appears to have evolved with bacteria as they are
ubiquitous in nature. The aim of present study was isolation and enrichment of bacteriophages against
antibiotic resistant Pseudomonas aeruginosa isolates.
Materials and Methods: Clinical samples from patients hospitalizedin different wards of Ghaem Hospital
(Mashhad, Iran) were collected and 43 antibiotic-resistant Pseudomonas aeruginosa were obtained from the
samples. Appropriate lytic bacteriophages were isolated from refined sewage of the septic ward of the same
hospital and bacteriocidal effect of the bacteriophages was evaluated in both liquid and solid phases. All tests
were three times repeated in different bacteriophage concentrations under different conditions.The results
were analyzed using statistical mann-witney test.
Results: By using two methods solid and liquid phase the high concentration of prepared bacteriphages
showed complete bactericidal effects on antibiotic-resistant isolated bacteria (p<0.0001).
Conclusion: Our data showed the high efficacy of bacteriophages disinfectant effects and it was more
prominent in the higher concentration of the bacteriophages.
Type of Study:
Original Research Article |
Subject:
Medical Virology Received: 2013/11/22 | Accepted: 2013/11/22 | ePublished: 2013/11/22